Isolation and identification of<i>Vibrio campbellii</i>as a bacterial pathogen for luminous vibriosis of<i>Litopenaeus vannamei</i>

Wang, Liping; Chen, Yanwu; Huang, Hui; Huang, Zhongdong; Chen, Hua; Shao, Zongze

doi:10.1111/are.12191

Cited by 52 publications

(43 citation statements)

References 48 publications

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“…Vibrio are a potential pathogen for shrimp, and Vibriosis outbreaks result in heavy economic loss for shrimp farmers (Liu, Cheng, Hsu & Chen ). The majority OTU of the Vibrio group of J3 had 99.04% identity with Vibrio campbellii , a potential Vibrio pathogen for P. monodon (Haldar, Chatterjee, Sugimoto, Das, Chowdhury, Hinenoya, Asakura & Yamasaki ) and L. vannamei (Wang, Chen, Huang, Huang, Chen & Shao ). Thus, our results provide important insights that may help prevent potential pathogenic Vibrio disease in the late growth stages during shrimp culture.…”

Section: Resultsmentioning

confidence: 99%

Changes in the intestinal bacterial community during the growth of white shrimp,Litopenaeus vannamei

et al. 2014

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In this study, we documented the changes in the intestinal bacterial community at four stages in Litopenaeus vannamei: 14 days postlarvae (L14) and 1‐, 2‐ and 3‐month old juveniles (J1, J2, J3), using 454 pyrosequencing techniques. The intestinal bacterial community was dominated by three bacterial phyla, Proteobacteria, Bacteroidetes and Actinobacteria at all stages. However, the relative abundance and bacterial lineages varied at the family level. The intestinal bacterial community of L14 and J1 was similar, with dominant members belonging to the Comamonadaceae of Betaproteobacteria. Conversely, bacterial members affiliated to Flavobacteriaceae of Bacteroidetes were dominant in J2 and Vibrionaceae of Gammaproteobacteria was dominant in J3. The abundance of Microbacteriaceae of Actinobacteria also fluctuated during the four stages. Bacterial members of Flavobacteriaceae and Rhodobacteraceae (Alphaproteobacteria) were present through all growth stages, and likely form the intestinal core microbiome of L. vannamei. However, they varied at the operational taxonomic unit (OTU) level through the growth stages. The intestinal bacterial community of pond‐rearing shrimp included the three main bacterial phyla identified above, and an additional group, Mycoplasmataceae of Mollicutes. Our results demonstrate that the intestinal bacterial community of L. vannamei was highly dynamic during the growth stages. Bacterial members belonging to Commamonadaceae dominated in the earlier growth stage of shrimp, possibly influenced by feeding with Artemia nauplii, but there was a shift to Flavobacteriaceae in the mid and Vibrionaceae in the late growth stages.

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Section: Resultsmentioning

confidence: 99%

Changes in the intestinal bacterial community during the growth of white shrimp,Litopenaeus vannamei

et al. 2014

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“…; Wang et al . ). In the present study, a Vibrio strain of the Harveyi clade B4F was isolated from seawater of an abalone farm.…”

Section: Discussionmentioning

confidence: 97%

“…V. harveyi and its phylogenetic neighbours V. campbellii , V. parahaemolyticus and V . alginolyticus have been demonstrated to be pathogenic for many aquatic organisms including shrimp, molluscs and fish (Liu, Chen, Huang & Lee ; Austin & Zhang ; Haldar, Chatterjee, Sugimoto, Das, Chowdhury, Hinenoya, Asakura & Yamasaki ; Sharma, Rathore, Verma, Sadhu & Philipose ; Wang, Chen, Huang, Huang, Chen & Shao ). Virulence of Vibrio spp.…”

Section: Introductionmentioning

confidence: 99%

Characterization of two bacteriophages for specific treatment of biofilm formed by aVibriosp. isolated from an abalone farm

Luo

Jost

et al. 2015

Aquac Res

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Vibrio harveyi and related vibrios are potential pathogens causing luminous vibriosis in marine aquaculture systems. In this study, two lytic phages P4A and P4F isolated using Vibrio strains B4A and B4F as indicator bacteria, respectively, were isolated from seawater of an abalone farm. Vibrio strain B4F belongs to the Harveyi clade of the genus Vibrio and was found to cause mortality of abalones in laboratory microcosms. Both phages were able to lyse Vibrio strain B4F. Electron microscopy revealed that phage P4A had an icosahedral head while P4F possessed an elongated hexagonal head. Both phages belong to the family Siphoviridae with long non‐contractile tails. Restriction endonuclease analysis indicated that both phages were double‐stranded DNA viruses and the genome sizes of P4A and P4F were estimated to be about 49 and 44 kb respectively. One‐step growth curves revealed that these two phages exhibited distinct latent periods, exponential periods and burst sizes by infecting the same Vibrio strain B4F. Both phages were able to significantly reduce Vibrio population density in biofilm formed by Vibrio strain B4F on the surface of polyethylene film. It is suggested that these two phages may be promising candidates as biocontrol agents of infections caused by Vibrio strains belonging to the Haveyi clade in marine aquaculture systems.

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“…Sementara Yano et al (2014) menemukan V. parahaemolyticus di lingkungan tambak udang resisten terhadap ampisilin dan oksitetrasiklin (72% dan 3%). Resistensi antibiotik dilaporkan terjadi pada beberapa spesies Vibrio di antaranya Vibrio cholera, V. harveyi, V. parahaemolyticus, V. vulnificus, V. fluvialis dan V. campbelli (Kang, Kim, Oh, Mok, & Cho, 2014;QuirozGuzman, Balcazar, Azquez-Juarez, Cruz-Villacorta, & Martínez-Díaz, 2013;Reboucas et al, 2011;Wang et al, 2015;Yano et al, 2014). Penggunaan senyawa antibiotik secara luas pada budidaya udang juga dapat menghasilkan infeksi pada manusia dan berpengaruh terhadap kesehatan manusia (European Federation of Animal Health, 1998;WHO, 2001 (Sunorita & Tjarsono, 2014).…”

Section: Pendahuluanunclassified

RESISTENSI ANTIBIOTIK PADA Vibrio parahaemolyticus DARI UDANG VANAME ASAL PANTAI UTARA JAWA UNTUK PASAR EKSPOR

Kusmarwati

Yenni

Indriati

2017

JPBKP

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ABSTRAKKeberadaan bakteri V. parahaemolyticus pada produk udang yang bersifat resisten terhadap antibiotik saat ini menjadi permasalahan serius yang berdampak pada jaminan mutu dan keamanan produk. Hal ini menjadi ancaman serius bagi manusia ketika mengkonsumsi udang tersebut. Penelitian ini bertujuan untuk mengetahui resistensi antibiotik dan potensi risiko antibiotik dari bakteri V. parahaemolyticus pada udang vaname. Pengambilan sampel udang vaname segar dari tambak dilakukan pada musim hujan dan musim kemarau di wilayah Pantai Utara Jawa pada bulan Februari hingga Oktober 2015 dengan metode purposive random sampling. Sebanyak 36 isolat bakteri V. parahaemolyticus yang mewakili 103 sampel udang vaname segar dari tambak udang di Jawa Barat, Jawa Tengah dan Jawa Timur telah diuji resistensinya terhadap 8 jenis antibiotik (doksisiklin, nitrofurantoin, siprofloksasin, asam nalidiksat, amoksisilin-asam klavulanat, kloramfenikol, streptomisin, dan eritromisin). Uji kepekaan bakteri terhadap antibiotik dilakukan menggunakan metode Kirby-Bauer. Perhitungan indeks Multiple Antibiotic Resistance (MAR) juga dilakukan untuk mengetahui potensi risiko antibiotik terhadap kesehatan manusia. Hasil analisis menunjukkan bahwa 100% isolat bakteri yang diuji resisten terhadap streptomisin, 90% isolat resisten terhadap eritromisin, dan berikutnya resisten terhadap amoksisilin-asam klavulanat dan nitrofurantoin masing-masing sebesar 83,33% dan 58,33%. Terdapat beberapa antibiotik yang masih mampu melawan bakteri V. parahaemolyticus yaitu siprofloksasin (88,89%), kloramfenikol (81,25%) dan doksisiklin (33,33%). Selain itu, sebanyak 63,89% dari total isolat bakteri tersebut memiliki indeks MAR>0,2 yang mengindikasikan adanya potensi risiko bagi kesehatan. Secara keseluruhan, bakteri V. parahaemolyticus dari sampel yang diambil pada musim hujan menunjukkan resistensi yang lebih tinggi. (doxycycline, nitrofurantoin, ciprofloxacin, nalidixic acid, amoxicillin-clavulanic acid, chloramphenicol, streptomycin, and erythromycin KATA KUNCI : multiple antibiotic resistance, udang, tambak, kloramfenikol ABSTRACT Recently, the occurence of V. parahaemolyticus bacteria in shrimp products that was resistant to antibiotics became a serious problem that affects quality assurance and product safety. It will be a serious threat to humans when consuming the shrimp product. Research was conducted to know antibiotic resistance and antibiotic risk potency of V. parahaemolyticus bacteria on vannamei shrimp. Sampling of fresh vannamei shrimp from the ponds was conducted in rainy and dry season in the Northern Coast of Java from February to October 2015 with purposive randomize sampling method. A total of 36 isolates of V. parahaemolyticus bacteria representing 103 samples of fresh vannamei shrimp from shrimp ponds in West Java, Central Java and East Java have been tested for resistance to 8 types of antibiotics

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Isolation and identification ofVibrio campbelliias a bacterial pathogen for luminous vibriosis ofLitopenaeus vannamei

Cited by 52 publications

References 48 publications

Changes in the intestinal bacterial community during the growth of white shrimp,Litopenaeus vannamei

Changes in the intestinal bacterial community during the growth of white shrimp,Litopenaeus vannamei

Characterization of two bacteriophages for specific treatment of biofilm formed by aVibriosp. isolated from an abalone farm

RESISTENSI ANTIBIOTIK PADA Vibrio parahaemolyticus DARI UDANG VANAME ASAL PANTAI UTARA JAWA UNTUK PASAR EKSPOR

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