Characterization of two bacteriophages for specific treatment of biofilm formed by a<i>Vibrio</i>sp. isolated from an abalone farm

Luo, Zengwei; Yu, Yanping; Jost, Günter; Liu, Wenhua; Huang, Xiang-Ling; Gu, Li

doi:10.1111/are.12846

Cited by 14 publications

(9 citation statements)

References 33 publications

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“…Our results suggested that phages can be found in virtually all places where their related hosts exist. Several other investigations reported the presence of Vibrio-specific lytic phages in environmental water (shrimp ponds, aquaculture system, sea water and aquatic product market) from different regions of the world (Alagappan et al, 2010;Luo et al, 2016;Stalin and Srinivasan, 2016;Katharios et al, 2017;Srinivasan and Ramasamy, 2017;Yang M. et al, 2019).…”

Section: Origin and Morphology Of Phages Ba3 And Ca8mentioning

confidence: 99%

Isolation and Characterization of the Novel Phages vB_VpS_BA3 and vB_VpS_CA8 for Lysing Vibrio parahaemolyticus

Yang

Liang

Huang³

et al. 2020

Front. Microbiol.

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Accumulating evidence has indicated that the multiple drug resistant Vibrio parahaemolyticus may pose a serious threat to public health and economic concerns for humans globally. Here, two lytic bacteriophages, namely vB_VpS_BA3 and vB_VpS_CA8, were isolated from sewage collected in Guangzhou, China. Electron microscopy studies revealed both virions taxonomically belonged to the Siphoviridae family with icosahedral head and a long non-contractile tail. The double-stranded DNA genome of phage BA3 was composed of 58648 bp with a GC content of 46.30% while phage CA8 was 58480 bp with an average GC content of 46.42%. In total, 85 putative open reading frames (ORFs) were predicted in the phage BA3 genome while 84 were predicted in that of CA8. The ORFs were associated with phage structure, packing, host lysis, DNA metabolism, and additional functions. Furthermore, average nucleotide identity analysis, comparative genomic features and phylogenetic analysis revealed that BA3 and CA8 represented different isolates but novel members of the family, Siphoviridae. Regarding the host range of the 61 V. parahaemolyticus isolates, BA3 and CA8 had an infectivity of 8.2 and 36.1%, respectively. Furthermore, ∼100 plaque-forming units (pfu)/cell for phage BA3 and ∼180 pfu/cell for phage CA8 were determined to be the viral load under laboratory growth conditions. Accordingly, the phage-killing assay in vitro revealed that phage CA8 achieved approximately 3.65 log unit reductions. The present results indicate that CA8 is potentially applicable for biological control of multidrug resistant V. parahaemolyticus.

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Section: Origin and Morphology Of Phages Ba3 And Ca8mentioning

confidence: 99%

Isolation and Characterization of the Novel Phages vB_VpS_BA3 and vB_VpS_CA8 for Lysing Vibrio parahaemolyticus

Yang

Liang

Huang³

et al. 2020

Front. Microbiol.

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“…The phage titre was determined by a drop count method (Luo et al, 2016). Ten-fold serial dilutions of the phage suspension was prepared in PBS buffer.…”

Section: Bacteriophage Identification Purification and Titrationmentioning

confidence: 99%

Bacteriophage versus antibiotic therapy on gut bacterial communities of juvenile green turtle, Chelonia mydas

Ahasan

Kinobe

Elliott³

et al. 2019

Environmental Microbiology

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Summary Green turtles are endangered marine herbivorous hindgut fermenters that contribute to a variety of marine ecosystems. Debilitated turtles are often rehabilitated in turtle hospitals. Since accurate diagnosis of disease is difficult, broad‐spectrum antibiotics are routinely used as a general treatment, potentially causing collateral damage to the gut microbiome of the patient. Here, we evaluated the concept of the application of bacteriophage (phages) to eliminate targeted intestinal bacteria as an alternative to a broad‐spectrum antibiotic (enrofloxacin) in clinically healthy, captive green turtles. Additionally, the impact of a broad‐spectrum antibiotic (enrofloxacin) and phage therapy on the gut bacterial communities of green turtles was evaluated. Gut bacterial communities in faecal samples were analysed by sequencing the V1‐V3 regions of the bacterial 16S rRNA. Bacteria‐specific phage cocktails significantly (P < 0.05) reduced targeted Acinetobacter in phage‐treated turtles during the therapy. Compared to control, no significant difference was observed in the bacterial diversity and compositions in phage‐treated turtles. In contrast, bacterial diversity was significantly (P < 0.05) reduced in antibiotic‐treated turtles at day 15 and throughout the trial. The alteration in the bacterial microbiota of antibiotic‐treated turtles was largely due to an increase in abundance of Gram‐positive Firmicutes and a concurrent decrease in Gram‐negative Bacteroidetes, Proteobacteria and Verrucomicrobia. Additionally, we observed the relative abundance of several bacteria at lower taxonomic level was much less affected by phages than by antibiotics. These data offer the proof of concept of phage therapy to manipulate transient as well as indigenous bacterial flora in gut‐related dysbiosis of turtles.

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“…The results demonstrated that while phage treatment never fully prevented biofilm formation, biofilm biomass and cell density was significantly reduced ( Curtin and Donlan, 2006 ; Fu et al, 2010 ). Luo et al (2015) isolated two phages P4A and P4F which belong to the Siphoviridae family from seawater of an abalone farm and applied the phages to reduce Vibrio biofilm. In the study, the both phages were able to bring about 2 logs reduction in a Vibrio harveyi biofilm cell density after 24 h of phage treatment ( Luo et al, 2015 ).…”

Section: Advantages Of Phage Therapymentioning

confidence: 99%

“… Luo et al (2015) isolated two phages P4A and P4F which belong to the Siphoviridae family from seawater of an abalone farm and applied the phages to reduce Vibrio biofilm. In the study, the both phages were able to bring about 2 logs reduction in a Vibrio harveyi biofilm cell density after 24 h of phage treatment ( Luo et al, 2015 ).…”

Section: Advantages Of Phage Therapymentioning

confidence: 99%

Insights into Bacteriophage Application in Controlling Vibrio Species

et al. 2016

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Bacterial infections from various organisms including Vibrio sp. pose a serious hazard to humans in many forms from clinical infection to affecting the yield of agriculture and aquaculture via infection of livestock. Vibrio sp. is one of the main foodborne pathogens causing human infection and is also a common cause of losses in the aquaculture industry. Prophylactic and therapeutic usage of antibiotics has become the mainstay of managing this problem, however, this in turn led to the emergence of multidrug resistant strains of bacteria in the environment; which has raised awareness of the critical need for alternative non-antibiotic based methods of preventing and treating bacterial infections. Bacteriophages – viruses that infect and result in the death of bacteria – are currently of great interest as a highly viable alternative to antibiotics. This article provides an insight into bacteriophage application in controlling Vibrio species as well underlining the advantages and drawbacks of phage therapy.

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Characterization of two bacteriophages for specific treatment of biofilm formed by aVibriosp. isolated from an abalone farm

Cited by 14 publications

References 33 publications

Isolation and Characterization of the Novel Phages vB_VpS_BA3 and vB_VpS_CA8 for Lysing Vibrio parahaemolyticus

Isolation and Characterization of the Novel Phages vB_VpS_BA3 and vB_VpS_CA8 for Lysing Vibrio parahaemolyticus

Bacteriophage versus antibiotic therapy on gut bacterial communities of juvenile green turtle, Chelonia mydas

Insights into Bacteriophage Application in Controlling Vibrio Species

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