Isolation and identification of <i>Sydowia polyspora</i> and its pathogenicity on <i>Pinus yunnanensis</i> in Southwestern China

Pan, Yajie; Hui, Yang; Lü, Jun; Chen, Peng; Zhou, Xudong; Qiao, Min; Yu, Zefen

doi:10.1111/jph.12696

Cited by 22 publications

(35 citation statements)

References 37 publications

(79 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Two of the three isolates used in the inoculation tests did not cause necrosis on the shoots. Pan et al () also verified different degrees of symptoms development on needles of P. yunannensis inoculated with different S. polyspora strains, when four of the ten strains tested caused no symptoms. In addition, Talgø et al () verified no symptom development in one of the three tests performed with the same strains, thus, suggesting that diverse abiotic and biotic factors can play an important role in the development of the disease, either by affecting the ecology of the pathogen or the susceptibility of the host.…”

Section: Resultsmentioning

confidence: 85%

“…In order to test the pathogenicity of S. polyspora isolates on stone pine, inoculation was performed following a method based on Pan et al (). Three isolates, from different geographic origins, were randomly selected (MEAN1029, MEAN1030, MEAN1031).…”

Section: Methodsmentioning

confidence: 99%

“…Similar symptoms caused by S. polyspora are reported on Pinus spp. Pan et al () verified that S. polyspora was able to infect Pinus yunnanensis Franch. in Southwestern China, causing chlorotic and reddish brown coloured lesions on needles, randomly distributed amongst otherwise healthy needles.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

First report of Sydowia polyspora causing disease on Pinus pinea shoots

et al. 2019

View full text Add to dashboard Cite

The fungus Sydowia polyspora is frequently isolated from conifers worldwide and is considered a pathogen on several hosts. Stone pine (Pinus pinea) is one of the most important forestry species throughout the Mediterranean basin due to the value of the edible pine nut. Stone pines showing tip dieback, needles with tan‐ to yellow‐coloured lesions and shoot death, observed in stands in Portugal, were sampled for analysis. Fungal colonies covered with cream‐coloured spore masses, were consistently obtained. Morphological and phylogenetic analyses of the ITS rDNA region enabled identification of these isolates as S. polyspora. Inoculation tests showed that the fungus caused lesions on excised P. pinea shoots. The symptoms observed might have a negative effect on pine nut production, and thus, evaluation of the impact of this disease is of relevance to future research. This paper is the first to report S. polyspora causing disease on P. pinea.

show abstract

Section: Resultsmentioning

confidence: 85%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

First report of Sydowia polyspora causing disease on Pinus pinea shoots

et al. 2019

View full text Add to dashboard Cite

show abstract

“…across Europe and North America [90][91][92], necrosis on shoots of Pinus pinea L. [93] as well as necrosis on stems and needles on Pinus yunnanensis Franch. [94]. Cleary et al [24] suggested that this endophyte is opportunistic pathogen, that due changes in climate can potentially increase its pathogenicity.…”

Section: Main Species Of Pinus Twigsmentioning

confidence: 99%

The Opportunistic Pathogen Sphaeropsis Sapinea is Found to be one of the Most Abundant Fungi in Symptomless and Diseased Scots Pine in Central-Europe

Blumenstein¹,

Langer²,

Bußkamp³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: The opportunistic and latent pathogen Sphaeropsis sapinea is one of the most important forest pathogens on pine. The fungus may cause Diplodia tip blight on several coniferous trees and disease symptoms come visible when trees are weakened by stress, usually related to injuries and drought. This project compares the mycobiome of healthy and diseased Scots pines. Twigs were sampled in June and September 2018 in a German forest stand with varying health status of the sampled Scots pines. Growth of 2017 and 2018 were sampled and cultivable, filamentous fungi isolated and the mycobiome was analysed by high-throughput sequencing (HTS) of the internal transcribed spacer 2 (ITS2) region. Results: A PERMANOVA analysis confirmed that the mycobiome community composition significantly differed between growth years (p < 0.001) and sampling time (p < 0.001) but not between healthy and diseased trees. Higher amount of S. sapinea was observed in June and the growth year 2017. Besides yeasts which were neglected, 23 ascomyceteous fungal endophytes were isolated. S. sapinea was the most common endophyte isolated and the second common in HTS data. It was highly abundant in symptomless (healthy) trees. Conclusion: Results highlights the ability of S. sapinea to accumulate unnoticed before disease outbreaks, implementing the sudden threat for Scots pines in the future.

show abstract

“…Isolate morphology was assessed after 6 and 15 days of growth on PDA medium at 25 • C under the 12 h photoperiod. Anamorph characteristics, including the size and shape of conidiophores and conidia, were observed in water on a glass slide using an Olympus compound microscope (BX 53; Dongguan Yijiang Instrument Co. Ltd., Guangdong, China) [37]. Measurements of 50 conidia, including length and width, and 95% confidence intervals together with extreme values were determined [24].…”

Section: Morphological Identificationmentioning

confidence: 99%

Morphology, DNA Phylogeny, and Pathogenicity of Wilsonomyces carpophilus Isolate Causing Shot-Hole Disease of Prunus divaricata and Prunus armeniaca in Wild-Fruit Forest of Western Tianshan Mountains, China

Jia

GuiFang³

et al. 2020

Forests

View full text Add to dashboard Cite

Prunus divaricata and Prunus armeniaca are important wild fruit trees that grow in part of the Western Tianshan Mountains in Central Asia, and they have been listed as endangered species in China. Shot-hole disease of stone fruits has become a major threat in the wild-fruit forest of the Western Tianshan Mountains. Twenty-five isolates were selected from diseased P. divaricata and P. armeniaca. According to the morphological characteristics of the culture, the 25 isolates were divided into eight morphological groups. Conidia were spindle-shaped, with ovate apical cells and truncated basal cells, with the majority of conidia comprising 3–4 septa, and the conidia had the same shape and color in morphological groups. Based on morphological and cultural characteristics and multilocus analysis using the internal transcribed spacer (ITS) region, partial large subunit (LSU) nuclear ribosomal RNA (nrRNA) gene, and the translation elongation factor 1-alpha (tef1) gene, the fungus was identified as Wilsonomyces carpophilus. The 25 W. carpophilus isolates had high genetic diversity in phylogenetic analysis, and the morphological groups did not correspond to phylogenetic groups. The pathogenicity of all W. carpophilus isolates was confirmed by inoculating healthy P. divaricata and P. armeniaca leaves and fruits. The pathogen was re-isolated from all inoculated tissues, thereby fulfilling Koch’s postulates. There were no significant differences in the pathogenicity of different isolates inoculated on P. armeniaca and P. divaricata leaves (p > 0.05). On fruit, G053 7m3 and G052 5m2 showed significant differences in inoculation on P. armeniaca, and G010 5m2 showed extremely significant differences with G004 7m2 and G004 5m2 on P. divaricata (p < 0.05). This is the first report on shot-hole disease of P. armeniaca (wild apricot) leaves and P. divaricata induced by W. carpophilus in China.

show abstract

Isolation and identification of Sydowia polyspora and its pathogenicity on Pinus yunnanensis in Southwestern China

Cited by 22 publications

References 37 publications

First report of Sydowia polyspora causing disease on Pinus pinea shoots

First report of Sydowia polyspora causing disease on Pinus pinea shoots

The Opportunistic Pathogen Sphaeropsis Sapinea is Found to be one of the Most Abundant Fungi in Symptomless and Diseased Scots Pine in Central-Europe

Morphology, DNA Phylogeny, and Pathogenicity of Wilsonomyces carpophilus Isolate Causing Shot-Hole Disease of Prunus divaricata and Prunus armeniaca in Wild-Fruit Forest of Western Tianshan Mountains, China

Contact Info

Product

Resources

About