First report of <i>Sydowia polyspora</i> causing disease on <i>Pinus pinea</i> shoots

Silva, Ana Cristina; Henriques, Joana; Diogo, Eugénio; Ramos, Ana Paula; Bragança, Helena

doi:10.1111/efp.12570

Cited by 10 publications

(18 citation statements)

References 7 publications

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“…However, it has been noted to cause current season needle necrosis (CSNN) in true r (Abies spp.) across Europe and North America [90][91][92], necrosis on shoots of Pinus pinea L. [93] as well as necrosis on stems and needles on Pinus yunnanensis Franch. [94].…”

Section: Main Species Of Pinus Twigsmentioning

confidence: 99%

The Opportunistic Pathogen Sphaeropsis Sapinea is Found to be one of the Most Abundant Fungi in Symptomless and Diseased Scots Pine in Central-Europe

Blumenstein¹,

Langer²,

Bußkamp³

et al. 2020

Preprint

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Background: The opportunistic and latent pathogen Sphaeropsis sapinea is one of the most important forest pathogens on pine. The fungus may cause Diplodia tip blight on several coniferous trees and disease symptoms come visible when trees are weakened by stress, usually related to injuries and drought. This project compares the mycobiome of healthy and diseased Scots pines. Twigs were sampled in June and September 2018 in a German forest stand with varying health status of the sampled Scots pines. Growth of 2017 and 2018 were sampled and cultivable, filamentous fungi isolated and the mycobiome was analysed by high-throughput sequencing (HTS) of the internal transcribed spacer 2 (ITS2) region. Results: A PERMANOVA analysis confirmed that the mycobiome community composition significantly differed between growth years (p < 0.001) and sampling time (p < 0.001) but not between healthy and diseased trees. Higher amount of S. sapinea was observed in June and the growth year 2017. Besides yeasts which were neglected, 23 ascomyceteous fungal endophytes were isolated. S. sapinea was the most common endophyte isolated and the second common in HTS data. It was highly abundant in symptomless (healthy) trees. Conclusion: Results highlights the ability of S. sapinea to accumulate unnoticed before disease outbreaks, implementing the sudden threat for Scots pines in the future.

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Section: Main Species Of Pinus Twigsmentioning

confidence: 99%

The Opportunistic Pathogen Sphaeropsis Sapinea is Found to be one of the Most Abundant Fungi in Symptomless and Diseased Scots Pine in Central-Europe

Blumenstein¹,

Langer²,

Bußkamp³

et al. 2020

Preprint

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“…Fuckel [43,44], and has recently also been associated with Sydowia polyspora (Bref. & Tavel) E. Müller [45]. In the present study, various Pestalotiopsis species were isolated from stone pine samples with similar symptoms, moreover, Pe.…”

Section: Discussionmentioning

confidence: 53%

“…In fact, D. sapinea, S. polyspora and other fungi were also present in some of the sampled symptomatic material (data not shown). Diverse authors also report more than one species involved in dieback and blight diseases, including pestalotioid species and other fungi [8,[45][46][47] and observed that some abiotic factors also have a major role in disease development, namely water stress and air temperature [41,42,47]. In this case, a synergic effect among Pe.…”

Section: Discussionmentioning

confidence: 98%

Pestalotiopsis pini sp. nov., an Emerging Pathogen on Stone Pine (Pinus pinea L.)

Silva

Diogo

Henriques

et al. 2020

Forests

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Research Highlights: Pestalotiopsis pini sp. nov. is an emerging pathogen on stone pine, Pinus pinea L., in Portugal. Background and Objectives: Stone pine is one of the most important forest tree species in Portugal and in the whole Mediterranean basin. Pestalotiopsis species are common endophytes, saprobes or pathogens in a variety of hosts and environments. The objective of the present study was to identify the Pestalotiopsis species associated with the symptomatic stone pine trees. Materials and Methods: Samples of stone pine trees showing shoot blight and stem necrosis were obtained from stone pine orchards and urban areas in Portugal, and the isolated Pestalotiopsis species were identified based on morphology and combined ITS, TEF and TUB DNA sequence data. Artificial inoculations on one-year-old stone pine seedlings were performed with the two species most frequently found in association with shoot blight disease. Results: Five Pestalotiopsis spp. were isolated. A taxonomic novelty, Pestalotiopsis pini is described, representing a new pathogen for stone pine. Conclusions: Pestalotiopsis species may represent a threat to the health of pine forests in the Mediterranean basin. Future research should be done in order to increase our knowledge about the potential impact of pestalotioid species in stone pine, in order to develop management strategies against these pathogens.

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“…Adicionalmente, y aunque para Sydowia polyspora solo se hallaron reportes de identificación molecular con ITS (Talgø et al 2010, Thambugala et al 2014, Silva et al 2020, es importante ratificar su identidad debido a que no ha sido reportado con anterioridad como patógeno de fresa. Este hongo ha sido mayormente asociado a especies de coníferas (Pan et al 2018), que ha causado daños en plantaciones forestales.…”

Section: Discussionunclassified

Patogenicidad de hongos asociados a plantas de fresa (Fragaria ananassa) y descripción ultraestructural del patosistema

Montero

Castañeda²,

Echandi³

et al. 2022

RAC

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Introducción. El cultivo de fresa (Fragaria ananassa) es importante en Costa Rica por su alta demanda como fruta fresca y para elaboración de mermeladas. En 2015 empezaron reportes de muerte de plantas, presumiblemente, asociada a complejos de hongos habitantes en el suelo. Los síntomas eran enrojecimiento y necrosis de hojas, coronas, raíce y luego, el desplome de las plantas. Con pérdidas hasta del 90% en plantaciones nuevas. Objetivo. Determinar la patogenicidad de hongos asociados a plantas de fresa enfermas y describir la micro morfología del patosistema. Materiales y métodos. Se colectaron plantas enfermas variedad Festival, y se realizaron aislamientos a partir de corona y raíz. Se clasificaron los morfotipos recuperados, se eligieron 3 para secuenciación del espaciador interno transcrito del ADN nuclear ribosomal (nrDNA ITS por sus siglas en inglés) y ejecución de los Postulados de Koch, con 3,5 x 105 conidios/ml inoculados en gránulos de alfalfa. Las plantas se mantuvieron 19 semanas en ambiente protegido. Luego, se realizó el reaislamiento y la secuenciación de los hongos inoculados y se describió el patosistema por medio de microscopía electrónica de barrido. Resultados. Se obtuvieron 10 aislamientos de hongos de corona y 22 de raíz, que se clasificaron en 9 morfotipos. Se eligieron 3 que de acuerdo con el ITS, se ubicaron en las unidades taxonómicas operativas Fusarium oxysporum, Neonectria/Dactylonectria y Sydowia polyspora. Se registró 100% de incidencia en todas las plantas inoculadas y los síntomas coincidieron con los de campo, mientras que el testigo permaneció sano. Los porcentajes de similitud de las secuencias de ITS de los hongos aislados de plantas enfermas provenientes de campo y de los hongos recuperados de la prueba de patogenicidad, fueron > 99%. A nivel ultraestructural se observó que las raíces pierden la epidermis y son colonizadas por los hongos. Conclusión. Los hongos inoculados fueron patogénicos a plantas de fresa variedad Festival; además, tuvieron la capacidad de dañar la epidermis radical y multiplicarse internamente.

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First report of Sydowia polyspora causing disease on Pinus pinea shoots

Cited by 10 publications

References 7 publications

The Opportunistic Pathogen Sphaeropsis Sapinea is Found to be one of the Most Abundant Fungi in Symptomless and Diseased Scots Pine in Central-Europe

The Opportunistic Pathogen Sphaeropsis Sapinea is Found to be one of the Most Abundant Fungi in Symptomless and Diseased Scots Pine in Central-Europe

Pestalotiopsis pini sp. nov., an Emerging Pathogen on Stone Pine (Pinus pinea L.)

Patogenicidad de hongos asociados a plantas de fresa (Fragaria ananassa) y descripción ultraestructural del patosistema

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