Isolation and Identification ofcis-Zeatin Riboside from Tubers of Sweet Potato (Ipomoea batatasL.)

Abstract

cis-Zeatin riboside was isolated and identified as its trimethylsilyl derivative from tubers of sweet potato by solvent extraction, ion-exchange chromatography, high performance liquid chromatography, Amaranthus betacyanin bioassay, and mass spectrometry. The level was estimated from the result of bioassays.

Mass spectrometric determination of ribosyl trans-zeatin from sweet potato tubers (Ipomoea batatas L. cv. Kohkei No. 14).

“…Consequently we have so far identified cis-RZ, trans-RZ and IPG as the major cytokinins of sweet potato tubers. The level of each endogenous cytokinin was estimated by either its peak height on HPLCor from total ion monitoring of GC-MS. IPG, trans-RZ and cis-RZ were estimated to be 10.21 p.%, 1.32^zg and 0.44ng per kg fresh weight of the tuber, respectively. It should be noted that the level of trans-RZ is approximately three times higher than that of cis-RZ in the tuber.…”

The tRNA-degradation pathway impacts the phenotype and metabolome of Arabidopsis thaliana: evidence from atipt2 and atipt9 knockout mutants

“…Although iPs and tZs are the most prevalent CKs in various developmental processes of land plants, they are frequently not the predominant forms, and the physiological regulation of iPs/tZs compared to cZs varies considerably among species, tissues, and adaptive responses. For example, in some plants including sweet potato (Hashizume et al 1982), rice (Takagi et al 1985), potato (Nicander et al 1995), chickpea (Emery et al 1998), maize (Veach et al 2003), pea (Quesnelle and Emery 2007), and moss (Lindner et al 2014), cZs are the dominant CK forms. Recently, the role of cZ-type CKs in the regulation of plant abiotic stress tolerance, and resistance to pathogens and herbivory has been emphasized (Schäfer et al 2015b) while another study postulated that cisCKs act in a slower, weaker, yet persistent manner when compared to more pronounced, short-lived effects of transCKs (Hluska et al 2021).…”

Isopentenyltransferases as master regulators of crop performance: their function, manipulation, and genetic potential for stress adaptation and yield improvement

“…Based on the combined unique transcriptional activities of these two known CTK biosynthesis pathways, together with knowledge from Arabidopsis mutant studies, a hypothesis was formed that tRNA-IPT genes and the associated cZ-type CTKs mainly play housekeeping roles, while ATP/ADP-IPT genes and the associated iP/tZ-type CTKs may be involved in regulating organ development and responses to environmental stresses (K€ ollmer et al, 2014;Miyawaki et al, 2004Miyawaki et al, , 2006Wang et al, 2020a) (Figure 1c). However, cZs are highly abundant in many plants (Sch€ afer et al, 2015), and they are the predominant CTKs in sweet potato (Hashizume et al, 1982), rice (Takagi et al, 1985), potato (Nicander et al, 1995), chickpea (Emery et al, 1998, maize (Veach et al, 2003), pea (Quesnelle and Emery, 2007) and moss (Lindner et al, 2014). They are also the predominant CTKs, at certain developmental stages and in response to environmental cues (Sch€ afer et al, 2015).…”