Mass spectrometric determination of ribosyl trans-zeatin from sweet potato tubers (Ipomoea batatas L. cv. Kohkei No. 14).

Abstract

The presence of ribosyl trans-zeatin was determined in sweet potato tubers by means of solvent extraction, ionexchange chromatography, high performance liquid chromatography, Amaranthus betacyanin bioassay, and mass spectrometry. The level was estimated to be 1.32^g/kg fresh weight of the tuber.In previous papers, we have reported the presence and level of cis-RZ1] and IPG2) in sweet potato tubers. We have now found the presence of trans-RZ by means of GC-MS, HPLC, and the Amaranthus betacyanin bioassay froman extract of the tubers. In this communication, we wish to report the isolation and identification of trans-RZ from sweet potato tubers, as well as the levels of other cytokinins found in the tubers.

Physiological and Anatomical Characterization of a Late-storage Root-forming Mutant of Sweetpotato

“…5). This is consistent with previous reports (Matsuo et al, 1983(Matsuo et al, , 1988Nakatani and Komeichi, 1991;Sugiyama and Hashizume, 1989;Suye et al, 1983). In contrast, the increase in root ZR levels in 'KM95-A68' was delayed, and the degree of increase was less (Fig.…”

“…Plant hormones are often involved in regulation of plant development. Many reports have suggested involvement of cytokinins in regulation of storage root formation in sweetpotato (Matsuo et al, 1983(Matsuo et al, , 1988McDavid and Alamu, 1980;Nakatani, 1994;Nakatani and Komeichi, 1991;Nakatani and Matsuda, 1992;Spence and Humphries, 1972;Sugiyama and Hashizume, 1989;Suye et al, 1983). Endogenous ZR, the major cytokinin in sweetpotato (Matsuo et al, 1983;Sugiyama and Hashizume, 1989;Suye et al, 1983), was assayed in the present study.…”

“…Physiologically, cytokinins, especially t-zeatin riboside (ZR), may regulate storage root formation in sweetpotato (Matsuo et al, 1983(Matsuo et al, , 1988Nakatani and Komeichi, 1991;Sugiyama and Hashizume, 1989;Suye et al, 1983). A rapid increase in endogenous ZR occurs in sweetpotato roots simultaneously with storage root formation.…”