Isolation and Identification of Fungi From Strawberry Seedlings and its Biological Agent and Fungicides

Abstract

Results of isolated fungi from seedlings of three Strawberry varieties Sweet Charlie, Hapil and Festival showed that the root zone in the three varieties contained the largest number of fungi. The fungus F. culmorum was the highest existence in three areas roots and runners and crown. The isolation percentage was 66.15% in the runners zone of Hapil and 36.15 % in the root of Sweet Charlie, while the existence percentage of the fungus above was 55.76% in the crown area and 35.79% in the root zone of Festival, while the fungi Cylindrocarpon spp. appeared at 38.60 % in runners of sweet charlie and 35.91% in the root of Hapil, while fungus isolated from Festival in 23.60% in root zone, and the lowest rate was in fungus A. alternata in the root of sweet charlie with 2.32% and the fungus Phytophthora spp. in Hapil. In Festival fungi St. herbarum appeared lowest ratio 3.47 % in the root, the different percentages for the isolation of the fungi differed with the differentiation of varieties studies of Strawberry plant and isolation areas. The testing biological agent Trichoderma harzianum Indicates that his high efficiency against fungi F. culmorum and Cylindrocarpon spp. and Bipolaris spp. its degree antagonistic to 2, 2 and 2 respectively. Inhibition percentage was 88.88% and 87.77 % and 86%, respectively, for three fungi. For ability of bacterial biological agent Pseudomonas aeruginosa showed significant inhibition to the growth of three fungi. The study of the bacterial biological agent and fungus Cylindrocarpon spp. showed significant inhibition as well as bacterial biological agent and fungus Bipolaris spp. with inhibition percentage 100% Finally the results showed that the fungicides Azadirachtin in concentration 5cm 3 /liter inhibited significantly the growth of three fungi, and the highest percentage inhibition was with the treatment of fungicides and fungi Cylindrocarpon spp. with inhibition percentage 80 % while the lowest percentage of inhibition was 21.87% in Fungi F. culmorum.

Isolation and identification of phenolic compounds from Eugenia caryophyllus and study on its biological effect against Macrophomina phaseolina

“…3. These results agreed with previous studies in terms of isolation and diagnosis (Al-Handel et al,2021;Al-Ameri et al,2018).…”

“…With sterile distilled water and dried using sterile filter paper, all pieces were transferred to a Petri dish containing antibiotic-added PDA medium and incubated at a temperature of 25 ± 2 °C for five days. The fungi were purified by transferring part of the end of the fungus colony to another plate containing the food medium (PDA) and it was done under the same conditions (Agrios,2005, Al -Ameri et al,2018. M. phaseolina was isolated based on taxonomic keys (Ellis,1971, Leslie et al,2006, Pitt et al,2009.…”

Phytochemical Analysis of Terpenes from Eucalyptus camaldulensis and Study of its Biological Effect Against Macrophomina spp.

“…Sterile distilled and dried with sterile filter papers and the pieces were transferred with sterile forceps at the rate of ten pieces for each dish of sterile plastic Petri dishes with a diameter of 8.5 cm containing potato agars and sterile PDA dextrose. The dishes were incubated at a temperature of 25 C for five days [16,17]. All parts that showed fungal growth were examined under the minimum and maximum power of the compound microscope.…”