Isolation and identification of four flavonoid constituents from the seeds of Oroxylum indicum by high-speed counter-current chromatography

Cytotoxicity, Apoptosis Induction and Anti-Metastatic Potential of Oroxylum indicum in Human Breast Cancer Cells

“…However, such studies were performed using polar extracts unlike our study using non-polar extracts from the same plant. Previous phytochemical studies of O. indicum led to the identification of ellagic acid (Maitreyi et al, 2008), 5,7-Dihydroxy flavone (chrysin) (Babu et al, 2006), 5-hydroxy-8-methoxy-7-0-β-Dglucopyranuronosyl flavone (Nair & Joshi, 1979), Stigmast-5-en-3-ol (Rasadah et al, 1998), 5,6,7-trihydroxy flavone (baicalein) (Chen et al, 2003;Chen et al, 2005), 4',5-Dihydroxy-7-methoxy isoflavone (pratensol) (Polya, 2003), 3-(4-hydroxy phenyl)-2-propenoic acid and 3, 4', 5, 7-tetrahydroxyflavonol (Islam et al, 2010). However, the antineoplastic effects of the crude extract of O. indicum have hitherto not been extensively studied and can have significance since there could be a synergy between the different phytochemical constituents, which may have been undetected in other studies.…”

Effect of Additives on Micropropagation of an Endangered Medicinal Tree Oroxylum indicum L. Vent

“…It is valued for its antimicrobial, antiarthritic, anticancerous and antihepatitic properties possessed by its various parts. Root extract of this tree has been used for long in ayurvedic preparations like Dashmularisht and Chyawanprash [3,4] .This tree possesses a flavonoid viz. Baicalein used to check proliferation of human breast cancer cell line MDA -MB -435 [5] .…”

Countercurrent chromatography in analytical chemistry (IUPAC Technical Report)

“…The lower phase of a chloroform-methanol-water (volume ratio Countercurrent chromatography 369 13:7:8) system was used as the mobile phase, and baseline peak resolution of the four compounds was achieved in less than 30 min [44,45]. Several flavonoids, including 7-O-glucosylbaicalein, baicalein, and chrysin, were separated from an ethyl acetate extract of the seeds of Oroxylum indicum (a herbal medicine) using a column of 27-50 mL total volume by two-phase solvent systems chloroform-methanol-water (8:10:5) [46] and hexane-ethyl acetate-methanol-water at various volume ratios [47]. All these constituents were identified and detected by NMR spectroscopy and electrospray ionization/mass spectrometry (ESI/MS) after isocratic or step-gradient elution.…”