Isolation and identification of duck egg-borne bacteria and their antibiogram profile

Ema, Fateha Akther; Arif, Mohommad; Islam, Md. Ariful; Khatun, Mst. Minara

doi:10.5455/javar.2018.e253

Cited by 5 publications

(6 citation statements)

References 24 publications

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“…Multiple S. aureus outbreaks due to chicken meat, both raw and frozen, in Bangladesh, was previously recorded which varied from 24.56-95.83% [29,24,35]. We also found S. aureus infection in eggshell 84%, however, past data reported by authors from Bangladesh showed a higher prevalence of S. aureus from hen egg (66.67%) and duck egg (75%) in the local market [36,37]. Percentage of resistance of S. aureus isolated from our poultry meat samples to Penicillin, Erythromycin, Tetracycline, Sulphamethoxazole and Nalidixic Acid were found to be 88.89%, 61.11%, 55.56%, 55.56% and 50%, respectively.…”

Section: Resultssupporting

confidence: 51%

Detection of Drug-resistant S. aureus from Poultry Samples Collected from Different Areas of Bangladesh

Akhi

Das

Banik

et al. 2019

MRJI

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Staphylococcus aureus is gram-positive cocci that can cause foodborne illness which can be transmitted by chicken meat, both raw and undercooked; consumption of which may cause infection and/or toxicity in consumers. This current study was conducted for the detection of the prevalence of S. aureus in three types of poultry samples which included chicken meat, chicken eggs and droppings. Samples were aseptically collected from different rural and urban areas of 8 districts of Bangladesh in triplicate collection method which was conducted in the Centre for Excellence, Department of Microbiology, Primeasia University, Dhaka, Bangladesh to detect the Multi Drug-Resistant (MDR) S. aureus. Isolation was done by using 7.5% Sodium Chloride broth for enrichment and Mannitol salt agar after enriching samples in Buffer Peptone water. Antibiogram was done by using Kirby Bauer Method on Muller Hinton agar. The current study revealed 68% (17/25), 84% (21/25) and 52% (13/25) of S. aureus in chicken meat, eggs and dropping, respectively. Around 75.47% isolates (40/53) showed 100% similarity with S. aureus from the generated Dendrogram based on biochemical data. Cefixime (62.67%) showed the highest sensitivity against all of the isolates while Penicillin (86.62%) exhibited the highest resistance. Moreover, 59.62% of isolates were resistant to 5 or more drugs (Multidrug-Resistant). Poultry products such as meat and eggs were contaminated with drug-resistant S. aureus which can cause serious health effect to the consumer. The drug-resistant pathogen in droppings helps to indicate the spread of drug-resistant isolates in the environment. Therefore, proper sanitation measures should be taken to ensure the quality of the products during slaughtering, collecting eggs or disposing of poultry litter.

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Section: Resultssupporting

confidence: 51%

Detection of Drug-resistant S. aureus from Poultry Samples Collected from Different Areas of Bangladesh

Akhi

Das

Banik

et al. 2019

MRJI

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“…These study findings agree with the previous study conducted by Ema et al [ 37 ], Sultana et al [ 38 ], Martins et al [ 39 ], Singh et al [ 40 ], Tagoe et al [ 41 ], Pyzik and Marek [ 42 ], Sharma and Mazumdar [ 43 ], and Pekana and Green [ 44 ]. They found the highest resistance rate for Amoxicillin, Ampicillin, and Tetracycline against S. aureus .…”

Section: Resultssupporting

confidence: 93%

“…In Gram stain, the organism showed violet in color, Gram-positive, and cocci in shape and arranged in clusters resembling grapes under a light microscope; these characteristics were identical to those mentioned by Ema et al [ 37 ].…”

Section: Resultssupporting

confidence: 84%

Multidrug-resistant Staphylococcus aureus isolated from chicken nuggets sold at superstores in Mymensingh, Bangladesh

Ain¹,

Arif²,

Ema³

et al. 2022

J Adv Vet Anim Res

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Objective: This study was conducted to determine the colony forming units (CFU) to isolate, identify, and antibiotic sensitivity of Staphylococcus aureus from chicken nuggets (CN). Materials and Methods: Sixty CN samples from two brands were collected from different super¬stores in Mymensingh, Bangladesh. Uncooked, oven-cooked (OC), or gas stove-cooked (GSC) CN samples were inoculated onto mannitol salt agar and blood agar. Results: The total staphylococcal count (TSC) for uncooked CN ranged from log 4.68 to log 5.11 CFU/gm. For OC CN, TSC ranged from log 3.29 to log 3.62 CFU/gm. For GSC CN, TSC ranged from log 3.09 to log 3.49 CFU/gm. Relative to uncooked CN, microwave oven-cooked and GSC samples significantly reduced the TSC of CN (p < 0.01). Using the polymerase chain reaction assay and standard biochemical testing, only 8 out of 60 CN samples contained S. aureus. Staphylococcus aureus were resistant to Ampicillin (100%), Amoxicillin (100%), Oxacillin (75%), Cefixime (87.5%), Doxycycline (75%), intermediately sensitive to Erythromycin (25%), Cephalexin (12.5%), Ciprofloxacin (25%), Gentamicin (12.5%), Doxycycline (12.5%) and sensitive to Oxacillin (25%), Azithromycin (100%), Erythromycin (75%) Cephalexin (87.5%), Cefixime (12.5%), Chloramphenicol (100%), Ciprofloxacin (75%), Gentamicin (87.5%), Doxycycline (12.5%), and Vancomycin (100%). Conclusion: This study reports the first isolation and identification of S. aureus from CN in Bangladesh. GSC CN was better than OC and uncooked CN. Data also suggest that CN is contami¬nated with multidrug-resistant S. aureus, which poses a public health hazard.

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“…However, because RTE Available at www.veterinaryworld.org/Vol.15/June-2022/12.pdf meats have been identified as transporters for foodborne bacteria such as E. coli, these foods provide a significant microbiological risk [8]. Thus, food-borne illnesses comprise various diseases responsible for causing morbidity and mortality worldwide [9]. As a result, food-traceability systems, particularly for meat and meat-derived products, are urgently needed to improve the quality of food-processing events and ensure safe food for final consumers [10].…”

Section: Introductionmentioning

confidence: 99%

Isolation, identification, and antibiogram studies of Escherichia coli from ready-to-eat foods in Mymensingh, Bangladesh

et al. 2022

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Background and Aim: Ready-to-eat (RTE) foods are widely used at home, restaurants, and during festivals in Bangladesh. So it is very important to investigate possible microbial contamination in RTE foods. Therefore, this study aimed to determine the total coliform count (TCC), isolate, identify, and characterize the Escherichia coli in RTE foods. The antimicrobial sensitivity of E. coli obtained from RTE foods was also performed using 12 commonly used antibiotics. Materials and Methods: A total of 100 RTE food samples were collected aseptically and comprised of ten samples each: Burger, pizza, sandwich, chicken roll, chicken meat loaf, chicken fry, salad vegetable, ice-cream, yogurt, and milkshake sold in Mymensingh city. Samples were inoculated onto Eosin methylene blue agar and incubated at 37°C for 24 h. Isolation and identification of bacteria were performed based on cultural, staining, and biochemical properties, followed by a polymerase chain reaction. Results: The TCC in Chicken meat loaf, burger, pizza, sandwich, salad vegetable ice-cream, and yogurt samples were 3.57 ± 0.96, 3.69 ± 0.08, 3.50 ± 0.60, 2.60 ± 0.20, 4.09 ± 0.29, 4.44 ± 0.25, and 3.14 ± 0.30 mean log colony-forming units ± standard deviation/mL, respectively. The study found a higher prevalence of E. coli in RTE salad vegetable products than in RTE meat and milk products. Forty percent of the mixed vegetable salad samples showed positive results for E. coli. Whereas E. coli prevalence in RTE meat and milk products was 20% and 16.7%, respectively. All the 21 isolates were subjected to antibiotic susceptibility test against 12 different antibiotics. It was observed that 46.1% were susceptible, 16.6% were intermediate, 46.1% were resistant, and 47.6% were multidrug-resistant (MDR) among seven different antibiotic classes. E. coli isolates were resistant to cephalexin, ceftazidime, oxytetracycline, and ampicillin and sensitive to gentamycin, followed by kanamycin, ceftriaxone, colistin, and enrofloxacin.. Conclusion: The study revealed that RTE foods are a serious issue from a public health point of view. To achieve a safer level of E. coli in RTE foods sold for human consumption, public food outlets must improve hygienic and good production procedures. Moreover, MDR E. coli in these foods pose serious public health threats.

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Isolation and identification of duck egg-borne bacteria and their antibiogram profile

Cited by 5 publications

References 24 publications

Detection of Drug-resistant S. aureus from Poultry Samples Collected from Different Areas of Bangladesh

Detection of Drug-resistant S. aureus from Poultry Samples Collected from Different Areas of Bangladesh

Multidrug-resistant Staphylococcus aureus isolated from chicken nuggets sold at superstores in Mymensingh, Bangladesh

Isolation, identification, and antibiogram studies of Escherichia coli from ready-to-eat foods in Mymensingh, Bangladesh

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