Isolation and Identification of Cytotoxic Compounds from Aeschynomene fascicularis, a Mayan Medicinal Plant

Caamal-Fuentes, Edgar; Peraza‐Sánchez, Sergio R.; Torres-Tapia, Luis W.; Moo-Puc, Rosa

doi:10.3390/molecules200813563

Cited by 31 publications

(13 citation statements)

References 22 publications

(23 reference statements)

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“…It is native to the tropical Africa and Madagascar, cultivated in Egypt, Java and South America [12], Plants of genus Aeschynomene are reported to exert hepatoprotective [13], antimicrobial [14], cytotoxic [15], antioxidant [16], anthelmintic [17] and anti-inflammatory activities [18]. Preliminary phytochemical studies among different Aeschynomene species revealed the presence of flavonoid glycosides [19], pterocarpans [20] and chalcones [21]. On other hand, only some kaempferol derivatives were isolated from A .…”

Section: Introductionmentioning

confidence: 99%

Metabolic profile and hepatoprotective effect of Aeschynomene elaphroxylon (Guill. & Perr.)

Hashem¹,

Salama²,

Mohammed³

et al. 2019

PLoS ONE

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Liver diseases are life-threatening and need urgent medical treatments. Conventional treatment is expensive and toxic, so the urge for nutraceutical hepatoprotective agents is crucial. This study is considered the first metabolic profile of Aeschynomene elaphroxylon (Guill. & Perr.) extracts of; flowers, leaves & bark adopting UPLC-Orbitrap HRMS analysis to determine their bioactive metabolites, and it was designed to investigate the potential hepatoprotective activity of A. elaphroxylon flowers and bark extracts against CCl4-induced hepatic fibrosis in rats. Forty-nine compounds of various classes were detected in the three extracts, with triterpenoid saponins as the major detected metabolite. Flowers and bark extracts presented similar chemical profile while leaves extract was quite different. The antioxidant activities of the flowers, leaves & bark extracts were measured by in vitro assays as Fe+3 reducing antioxidant power and Oxygen radical absorbance capacity. It revealed that flowers and bark extracts had relatively high antioxidant activity as compared to leaves extract. Based on the metabolic profile and in vitro antioxidant activity, flowers and bark ethanolic extracts were chosen for alleviation of hepatotoxicity induced by CCl4 in rats. The hepatoprotective activity was studied through measuring hepatotoxicity biomarkers in serum (ALT, AST, and Albumin). Liver tissues were examined histopathologically and their homogenates were used in determining the intracellular levels of oxidative stress biomarkers (MDA, GSH), inflammatory markers (TNF-α). Flowers and bark ethanolic extracts exerted a significant hepatoprotective effect through reduction in the activities of ALT, AST and Albumin, the tested extracts reduced oxidative stress by increasing GSH content and reducing the MDA level. Furthermore, the extracts decreased levels of pro-inflammatory TNF-α. Moreover, the present study revealed the potentiality of A. elaphroxylon in ameliorating the CCl4-induced hepatic fibrosis in rats. In this aspect, A. elaphroxylon can be used with other agents as a complementary drug.

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Section: Introductionmentioning

confidence: 99%

Metabolic profile and hepatoprotective effect of Aeschynomene elaphroxylon (Guill. & Perr.)

Hashem¹,

Salama²,

Mohammed³

et al. 2019

PLoS ONE

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“…R. communis seeds, leaves, fruit, stem and bark are used in different traditional therapeutic practices by local practitioner (Hakeem) [ 21 ]. Therefore, the toxicity profiling (using bioassays) of such important plants is very helpful to appraise the safety [ 22 – 33 ]. In this regard, the bioassays (hemolytic and brine shrimp) are the standard tests to evaluate the cytotoxicity, whereas TA98 and TA100 (based on salmonella mutant strains) are the reference tests for mutagenicity evaluation.…”

Section: Introductionmentioning

confidence: 99%

Mutagenicity, cytotoxic and antioxidant activities of Ricinus communis different parts

Abbas

Ali

Arshad

et al. 2018

Chemistry Central Journal

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Ricinus communis (castor plant) is a potent medicinal plant, which is commonly used in the treatment of various ailments. The present study was conducted to appraise the cytotoxicity and mutagenicity of R. communis along with antioxidant and antimicrobial activities. Cytotoxicity was evaluated by hemolytic and brine shrimp assays, whereas Ames test (TA98 and TA100) was used for mutagenicity evaluation. Plant different parts were extracted in methanol by shaking, sonication and Soxhlet extraction methods. The R. communis methanolic extracts showed promising antioxidant activity evaluated as through total phenolic contents (TPC), total flavonoid content (TFC), DPPH free radical inhibition, reducing power and inhibition of linoleic acid oxidation. R. communis seeds, stem, leaves, fruit and root methanolic extracts showed mild to moderate cytotoxicity against red blood cells (RBCs) of human and bovine. Brine shrimp lethality also revealed the cytotoxic nature of extracts with LC 50 in the range of 0.22-3.70 (µg/mL) (shaking), 1.59-60.92 (µg/mL) (sonication) and 0.72-33.60 (µg/mL) (Soxhlet), whereas LC 90 values were in the range of 345. 42-1695.81, 660.50-14,794.40 and 641.62-15,047.80 µg/mL for shaking, sonication and Soxhlet extraction methods, respectively. R. communis methanolic extracts revealed mild mutagenicity against TA98 (range 1975 ± 67 to 2628 ± 79 revertant colonies) and TA100 (range 2773 ± 92 to 3461 ± 147 revertant colonies) strains and these values were 3267 ± 278 and 4720 ± 346 revertant colonies in case of TA98 and TA100 positive controls, respectively. R. communis methanolic extracts prevented the H 2 O 2 and UV to Plasmid pBR 322 DNA oxidative damage. Results revealed that R. communis is a potential source of bioactive compounds and in future studies the bioactive compounds will be identified by advanced spectroscopic techniques.

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“…The percentage of growth inhibition was determined using a MTT colorimetric technique to measure four viable cells (HL-60 human myeloid leukemia, SMMC-7721 hepatocellular carcinoma, A-549 lung cancer and MCF-7 breast cancer) with minor modification [18,19]. A total of 5000-10,000 exponential phase cells per well were seeded onto a 96-well plate for 24 h, Each tumor cell line was exposed to a test compound at concentrations of 0.0624, 0.32, 1.6, 8, and 40 µM in DMSO in triplicate for 72 h, with cisplatin as the positive control.…”

Section: Anti-proliferative Activitymentioning

confidence: 99%

Bioactive Diterpenoids from Clerodendrum kiangsiense

Wang

Jia

et al. 2016

Molecules

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Abstract:A new abeo-abietane diterpenoid, 12-methoxy-6,11,14,16-tetrahydroxy-17(15Ñ16)-abeo-5,8,11,13-abietatetraen-3,7-dione (8), was isolated from the hydroalcoholic extract of the herb of Clerodendrum kiangsiense along with seven known diterpenoids (1-7). Their structures were identified on the basis of spectroscopic analyses including two-dimensional NMR and comparison with literature data. All of these compounds were evaluated for their cytotoxic activities against the growth of human cancer cells lines HL-60, SMMC-7721, A-549 and MCF-7 by the MTT assay. The results showed that cryptojaponol (4), fortunin E (6) and 8 exhibited significant cytotoxicity against four human cancer cell lines.

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Isolation and Identification of Cytotoxic Compounds from Aeschynomene fascicularis, a Mayan Medicinal Plant

Cited by 31 publications

References 22 publications

Metabolic profile and hepatoprotective effect of Aeschynomene elaphroxylon (Guill. & Perr.)

Metabolic profile and hepatoprotective effect of Aeschynomene elaphroxylon (Guill. & Perr.)

Mutagenicity, cytotoxic and antioxidant activities of Ricinus communis different parts

Bioactive Diterpenoids from Clerodendrum kiangsiense

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