Isolation and Identification of Coagulase-Negative Staphylococcus Species from Bovine Body Sites and Streak Canals of Nulliparous Heifers

White, D.G.; Harmon, Robert J.; Matos, J.E.S.; Langlois, B. E.

doi:10.3168/jds.s0022-0302(89)79307-3

Cited by 83 publications

(84 citation statements)

References 12 publications

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“…The average of staphylococcus load is up to 10 3 UCF / ml and is in equivalent tendency to that reported to Tadla region (encompassing Beni Mellal area) (up to 5 x 10 3 UCF / ml; [6]) and at lesser degree to that reported in Oujda city [4]. Staphylococcus species besides streptococcus species were the most commonly isolated bacteria in milk produced by animals with subclinical mastitis [18] and [19]. Our results which are slightly different from those of Afif et al [6]in the same area, show firstly that an effort has been made in the sense of ameliorating the quality of raw cow milk and secondly that they are in favor of a relative respect of hygienic procedures in the cooperatives investigated and this could be in part explained by the this can be explained by the necessity of the producers to respect the conditions of the manufacturers.…”

Section: Discussionsupporting

confidence: 50%

Microbiological Characteristics of Raw Cow Milk in Beni Mellal Area (Morocco)

Ferdous¹,

Hnini²,

Merzouki³

et al. 2017

IJSEA

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Although a great effort has been made in Morocco concerning the produced quantity of raw cow milk, its quality does not meet acceptable quality standards. Theobjective of this study was to evaluate the global milk quality in Moroccan dairy herds (hygienic and pathogenic parameters) and the perspectives on dairy development. For this, we evaluated the variations in the overall quality of raw milk: bacteriological and hygienic characterizations. Thus, samples of raw cow's milk produced in 10-12 farms were selected from different areas in Beni Mellal region. The investigation has concerned different cattle farms with different breeding procedures. Thus, microbiological characterization was evaluated in the samples analyzed for their standard plat count, total coliform and fecal coliform, yeast loads (L), molds (M) and some of pathogenic bacteria (staphylococcus, salmonella, listeria and clostridium). In general, the samples analyzed show a moderate to critical contamination of raw milk during different seasons in all microbial species analyzed, notably for fecal and total coliforms and total mesophilic aerobic flora (TMAF). As the microbiological quality of milk is critical to the health, organoleptic and technological qualities of milk, we assayed pathogenic bacteria and found a positivity only for staphylococcus aureus which exceeds the accepted limit. The most contaminated farms were those located essentially in the irrigated zones. The less contaminated ones belong to modern's farms. It seems that transportation, milking and pre-storage conditions as well as climate constitute the main basic relevant factors for heavy bacterial contamination Taken together these variations reflect essentially differences in practical breeding. When the period effect is investigated, differences between hot periods and cold-warm periods are present for the parameters mentioned above. These results indicate the non-respect of good hygiene practices as well as breeding practices.

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Section: Discussionsupporting

confidence: 50%

Microbiological Characteristics of Raw Cow Milk in Beni Mellal Area (Morocco)

Ferdous¹,

Hnini²,

Merzouki³

et al. 2017

IJSEA

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“…The colonisation of teat skin and its opening by CNS has been abundantly described in the literature (Boddie 1987, White et al 1989, Trinidad et al 1990). …”

Section: Discussionmentioning

confidence: 99%

Coagulase-negative staphylococci (CNS) as an aetiological factor of mastitis in cows

Bochniarz¹,

Wawron²,

Szczubiał³

2013

Polish Journal of Veterinary Sciences

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The aim of the present study was to determine the proportions of individual coagulase-negative Staphylococcus species in clinical and subclinical mastitis. The material consisted of 100 CNS isolates obtained from 223 milk samples collected from cows with clinical and subclinical mastitis. Coagulase-negative staphylococci constituted 44.8% of all isolated microorganisms. CNS were isolated from the mammary gland secretions of 86 cows from farms in the Lublin region (Poland). Clinical mastitis was found in 20 whereas subclinical mastitis in 66 study cows (23.3% and 76.7%, respectively). The symptoms of clinical mastitis were mild. The clinical forms of mastitis concerned mainly the first or second lactation. Subclinical mastitis was most commonly observed during the second lactation. Four CNS species (S. xylosus, S. chromogenes, S. haemolyticus and S. sciuri ) were isolated from clinical and subclinical mastitis. S. xylosus was the commonest CNS species isolated from cows with clinical mastitis whereas S. chromogenes was the most prevalent one in subclinical mastitis cases. The three CNS species (S. warneri, S. hominis and S. saprophyticus) caused only subclinical mastitis.

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“…The coagulase-negative staphylococci including Staphylococcus auricularis (60) were considered the most common bacteria from skin of various animals (18,53). Streptococcus bovis and Streptococcus equinus are other examples of species found on cow teat skin (49).…”

Section: Discussionmentioning

confidence: 99%

Cow Teat Skin, a Potential Source of Diverse Microbial Populations for Cheese Production

Verdier‐Metz¹,

Gagné

Bornes

et al. 2012

Appl Environ Microbiol

130

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The diversity of the microbial community on cow teat skin was evaluated using a culture-dependent method based on the use of different dairy-specific media, followed by the identification of isolates by 16S rRNA gene sequencing. This was combined with a direct molecular approach by cloning and 16S rRNA gene sequencing. This study highlighted the large diversity of the bacterial community that may be found on teat skin, where 79.8% of clones corresponded to various unidentified species as well as 66 identified species, mainly belonging to those commonly found in raw milk (Enterococcus, Pediococcus, Enterobacter, Pantoea, Aerococcus, and Staphylococcus). Several of them, such as nonstarter lactic acid bacteria (NSLAB), Staphylococcus, and Actinobacteria, may contribute to the development of the sensory characteristics of cheese during ripening. Therefore, teat skin could be an interesting source or vector of biodiversity for milk. Variations of microbial counts and diversity between the farms studied have been observed. Moreover, Staphylococcus auricularis, Staphylococcus devriesei, Staphylococcus arlettae, Streptococcus bovis, Streptococcus equinus, Clavibacter michiganensis, Coprococcus catus, or Arthrobacter gandavensis commensal bacteria of teat skin and teat canal, as well as human skin, are not common in milk, suggesting that there is a breakdown of microbial flow from animal to milk. It would then be interesting to thoroughly study this microbial flow from teat to milk.

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Isolation and Identification of Coagulase-Negative Staphylococcus Species from Bovine Body Sites and Streak Canals of Nulliparous Heifers

Cited by 83 publications

References 12 publications

Microbiological Characteristics of Raw Cow Milk in Beni Mellal Area (Morocco)

Microbiological Characteristics of Raw Cow Milk in Beni Mellal Area (Morocco)

Coagulase-negative staphylococci (CNS) as an aetiological factor of mastitis in cows

Cow Teat Skin, a Potential Source of Diverse Microbial Populations for Cheese Production

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