Coagulase-negative staphylococci (CNS) as an aetiological factor of mastitis in cows

Abstract

The aim of the present study was to determine the proportions of individual coagulase-negative Staphylococcus species in clinical and subclinical mastitis. The material consisted of 100 CNS isolates obtained from 223 milk samples collected from cows with clinical and subclinical mastitis. Coagulase-negative staphylococci constituted 44.8% of all isolated microorganisms. CNS were isolated from the mammary gland secretions of 86 cows from farms in the Lublin region (Poland). Clinical mastitis was found in 20 whereas subclinical mastitis in 66 study cows (23.3% and 76.7%, respectively). The symptoms of clinical mastitis were mild. The clinical forms of mastitis concerned mainly the first or second lactation. Subclinical mastitis was most commonly observed during the second lactation. Four CNS species (S. xylosus, S. chromogenes, S. haemolyticus and S. sciuri ) were isolated from clinical and subclinical mastitis. S. xylosus was the commonest CNS species isolated from cows with clinical mastitis whereas S. chromogenes was the most prevalent one in subclinical mastitis cases. The three CNS species (S. warneri, S. hominis and S. saprophyticus) caused only subclinical mastitis.

Application of Matrix-assisted Laser Desorption Ionization Time-of-flight Mass Spectrometry for Identification of Coagulase-negative Staphylococci Isolated from Milk of Cows with Subclinical Mastitis

“…The present results correspond to those of other authors, and suggest that coagulase-negative staphylococci are the main cause of mastitis in cows, while S. xylosus, S. chromogenes, S. haemolyticus and S. sciuri are the species most commonly isolated from the milk of diseased animals (Bochniarz et al 2013, Frey 2013. Staphylococcus equorum, S. warneri and S. lentus are less frequently present in such material according to the literature data (Anaya-López et al 2006, Bochniarz et al 2012.…”

“…For many decades, coagulase-negative staphylococci, widely spread in the natural environment and colonizing the skin and mucosa of animals and humans, have been considered non-pathogenic. At present, they are a predominant etiological factor for bovine mastitis in many countries (Taponen et al 2007, Pyörälä and Taponen 2009, Persson Waller et al 2011, Bochniarz et al 2013. CNS can cause clinical mastitis, although in the majority of cases they are responsible for subclinical mastitis (Taponen et al 2007, Persson Waller et al 2011.…”

Prevalence and etiological agents of subclinical mastitis at the end of lactation in nine dairy herds in North-East Poland

“…In the present study, most of the mastitis cases (31.6%) were caused by CNS. Similar results were reported from Poland by Malinowski et al (2006), Barański et al (2008) and Bochniarz et al (2013). CNS have become the predominant pathogen isolated from subclinical bovine mastitis in many countries, and could therefore be described as emerging mastitis pathogens (Myllys et al 1998, Pyörälä andTaponen 2009).…”

Detection of methicillin resistant and slime factor production of coagulase negative Staphylococcus spp. in bovine clinical mastitis by using PCR

“…At present, they are the predominant aetiological factor of bovine mastitis in many countries [2,21] .…”