Isolation and Identification of Associative Symbiotic N2 Fixing Microbes: Desulfovibrio

Mistry, Harsh; Thakor, Rashmi; Bariya, Himanshu

doi:10.1007/978-1-0716-1724-3_10

Cited by 2 publications

(2 citation statements)

References 8 publications

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“…Diazotroph Anaeromyxobacter correlated positively with

N and recently reported that Anaeromyxobacter enhances the nitrogen-fixing activity of paddy soils (Masuda et al, 2020 ). Desulfovibrio had a positive correlation with available

N. Desulfovibrio is known for its sulfur-reducing property and plays a particular and significant role in the fixation of biological nitrogen (Mistry et al, 2022 ). On the contrary, soil physiochemical, such as OM, showed a negative correlation with genera Desulfarculus and Anabaena , while

N showed a negative correlation with genera Methylobacter and Desulfarculus .…”

Section: Discussionmentioning

confidence: 99%

High-Throughput Sequencing-Based Analysis of Rhizosphere and Diazotrophic Bacterial Diversity Among Wild Progenitor and Closely Related Species of Sugarcane (Saccharum spp. Inter-Specific Hybrids)

Malviya

Lakshmanan

et al. 2022

Front. Plant Sci.

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Considering the significant role of genetic background in plant-microbe interactions and that most crop rhizospheric microbial research was focused on cultivars, understanding the diversity of root-associated microbiomes in wild progenitors and closely related crossable species may help to breed better cultivars. This study is aimed to fill a critical knowledge gap on rhizosphere and diazotroph bacterial diversity in the wild progenitors of sugarcane, the essential sugar and the second largest bioenergy crop globally. Using a high-throughput sequencing (HTS) platform, we studied the rhizosphere and diazotroph bacterial community of Saccharum officinarum L. cv. Badila (BRS), Saccharum barberi (S. barberi) Jesw. cv Pansahi (PRS), Saccharum robustum [S. robustum; (RRS), Saccharum spontaneum (S. spontaneum); SRS], and Saccharum sinense (S. sinense) Roxb. cv Uba (URS) by sequencing their 16S rRNA and nifH genes. HTS results revealed that a total of 6,202 bacteria-specific operational taxonomic units (OTUs) were identified, that were distributed as 107 bacterial groups. Out of that, 31 rhizobacterial families are commonly spread in all five species. With respect to nifH gene, S. barberi and S. spontaneum recorded the highest and lowest number of OTUs, respectively. These results were validated by quantitative PCR analysis of both genes. A total of 1,099 OTUs were identified for diazotrophs with a core microbiome of 9 families distributed among all the sugarcane species. The core microbiomes were spread across 20 genera. The increased microbial diversity in the rhizosphere was mainly due to soil physiochemical properties. Most of the genera of rhizobacteria and diazotrophs showed a positive correlation, and few genera negatively correlated with the soil properties. The results showed that sizeable rhizospheric diversity exists across progenitors and close relatives. Still, incidentally, the rhizosphere microbial abundance of progenitors of modern sugarcane was at the lower end of the spectrum, indicating the prospect of Saccharum species introgression breeding may further improve nutrient use and disease and stress tolerance of commercial sugarcane. The considerable variation for rhizosphere microbiome seen in Saccharum species also provides a knowledge base and an experimental system for studying the evolution of rhizobacteria-host plant association during crop domestication.

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“…Diazotroph Anaeromyxobacter correlated positively with

N and recently reported that Anaeromyxobacter enhances the nitrogen-fixing activity of paddy soils (Masuda et al, 2020 ). Desulfovibrio had a positive correlation with available

N showed a negative correlation with genera Methylobacter and Desulfarculus .…”

Section: Discussionmentioning

confidence: 99%

High-Throughput Sequencing-Based Analysis of Rhizosphere and Diazotrophic Bacterial Diversity Among Wild Progenitor and Closely Related Species of Sugarcane (Saccharum spp. Inter-Specific Hybrids)

Malviya

Lakshmanan

et al. 2022

Front. Plant Sci.

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show abstract

“…It has been reported that variation of niches might result in different interaction and functional role of the same organisms ( Liu et al, 2023 ). Some of the identified hub nodes such as Trichoderma , Pseudoalteromonas, Filobasidium, Sphingomonas, Rhizobacterium, Exiguobacterium , and Desulfovibrio have been reported as novel plant growth promoters through production of secondary metabolites, phytohormones, nitrogen fixation, and biological control ( Dastager et al, 2010 ; Nieto-Jacobo et al, 2017 ; Asaf et al, 2020 ; Chen et al, 2020 ; Navarro-Torre et al, 2020 ; Mistry et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Highly diverse microbial community of regenerated seedlings reveals the high capacity of the bulb in lily, Lilium brownii

Jibril,

Yan,

Wang

et al. 2024

Front. Microbiol.

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Lily bulbs, which have both nutrient storage and reproductive functions, are a representative group of plants for studying the maintenance and transfer of plant-associated microbiomes. In this study, a comparison of the microbial composition of bulbs and their regenerated seedlings cultured under aseptic conditions, as well as subcultured seedlings that succeeded five times, was examined by amplicon sequencing. A total of 62 bacterial taxa and 56 fungal taxa were found to be transferred to the 5th generation in seedlings, which are the core microbiome of lily. After the regeneration of seedlings from bulbs, there was a significant increase in the number of detectable microbial species, and after 1, 3, and 5 successive generations, there was a decrease in the number of detectable species. Interestingly, some “new” microorganisms appeared in each generation of samples; for instance, 167 and 168 bacterial operational taxonomic units (OTUs) in the 3rd and 5th generations of seedlings that were not detected in either bulbs or seedlings of the previous two generations. These results suggest that bulbs can maintain a high diversity of microorganisms, including some with ultra-low abundance, and have a high transfer capacity to tuck shoots through continuous subculture. The diversity and maintenance of the microbiome can provide the necessary microbial reservoir support for regenerating seedlings. This habit of maintaining low abundance and high diversity may be biologically and ecologically critical for maintaining microbiome stability and function due to the sequestration nature of the plant.

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Isolation and Identification of Associative Symbiotic N2 Fixing Microbes: Desulfovibrio

Cited by 2 publications

References 8 publications

High-Throughput Sequencing-Based Analysis of Rhizosphere and Diazotrophic Bacterial Diversity Among Wild Progenitor and Closely Related Species of Sugarcane (Saccharum spp. Inter-Specific Hybrids)

High-Throughput Sequencing-Based Analysis of Rhizosphere and Diazotrophic Bacterial Diversity Among Wild Progenitor and Closely Related Species of Sugarcane (Saccharum spp. Inter-Specific Hybrids)

Highly diverse microbial community of regenerated seedlings reveals the high capacity of the bulb in lily, Lilium brownii

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