Isolation and Identification of Antibiotic-Producing Halophilic Bacteria from Dagh Biarjmand and Haj Aligholi Salt Deserts, Iran

Abstract

Background: Halophilic bacteria are potent organisms in production of novel bioactive antimicrobial compounds which might be considered in drug innovation and control of plant pathogens. Salt deserts in Semnan province are of the most permanent hypersaline areas in the North of Iran. Despite the importance of these areas, there is no scientific report regarding the biodiversity and potency of their halophilic bacteria. Thus, aforementioned areas were selected to detect the halophilic bacteria. Methods: Here, seven strains were isolated and cultured on their molecular and biochemical properties were characterized. To determine the antibiotic potency of the isolates, agar well diffusion method was conducted. Phylogenetic analysis was done to reveal the isolates relationship with previously known strains. Results: As a result, growth of the strains in the medium containing 5 to 20% (w/v) NaCl determined that the majority of the isolates were moderately halophile. Catalase activity of all strains was positive. The results represented that D6A, Dar and D8B have antimicrobial effects against different plant and human pathogens. Phylogenic tree analysis also showed that two strains of D6A and Dar are belonged to Bacillus subtilis and D8B is belonged to Virgibacillus olivae. The bacteria extracts were evaluated for their antifungal and antibacterial activities on human and Plant pathogenic strains. The MIC of the extract B. subtilis against was found active against human pathogenic fungi and Plant pathogenic bacteria and fungi, ranging from 12.5 to 25 µg/mL. Conclusion: This study highlights the therapeutic and prophylactic potential of B. subtilis extracts as antibacterial and antifungal agents.

Antibacterial and Antifungal Activity of Novel Freshwater Bacterium Tabrizicola aquatica as a Prominent Natural Antibiotic Available in Qurugol Lake

“…The MIC value is detected as the lowest concentration for completely inhibition of the bacterial growth after 48 h. In addition, for detection of Pharmaceutical Sciences, 2020, 26(1), 88-92 | 90 the MIC rate against experimented pathogens, the various concentrations including (10 -1 g / ML to 10 -8 g / ml) were carried out according to described method by Elyasifar et al (2019). 16 Moreover, 5 μl of liquid from every well which exhibited no growth were taken and incubated for 24 h in the same condition for determination of MBC. The lowest concentration after sub-culturing that prevented no obvious bacterial growth was taken as MBC.…”

“…The lowest concentration after sub-culturing that prevented no obvious bacterial growth was taken as MBC. 16…”

“…The negative control is involved an antimicrobial extract and culture media and the positive controls including the mix of culture medium with 0.5 McFarlend of the pathogen. Moreover, the MFC was determined as the lowest concentration with three or fewer colonies incubated at 30 ⁰C for 72 h. 16…”

Polycyclic Aromatic Hydrocarbons Degradation by Aquatic Bacteria Isolated from Khazar Sea, the World’s Largest Lake

“…42 Elyasifar et al isolated halophilic bacterial belonged to Bacillus subtilis and Virgibacillus olivae, from Dagh Biarjmand and Haj Aligholi salt deserts in Iran with potential to produce antibacterial and antifungal agents. 43 Several aerobic naphthalene-degrading bacteria account for 86.9% of the hydrocarbon-degrading microorganisms found in gasoline-contaminated aquifers. 44 For the first time, Ben Said et al described the ability of Shewanella oneidensis species to degrade fluoranthene and pyrene as high molecular weight and, phenanthrene as a low molecular weight PAH.…”

Caracterización de bacterias halotolerantes aisladas del proceso de salado madurado de Engraulis ringens (Jenyns, 1842) “anchoveta”

“…Por tanto, a pesar de la similitud de características morfológicas, existen diferencias excepcionales que brindan al agente antimicrobiano capacidad de inhibir el crecimiento. De igual forma, Elyasifar et al (2019), al identificar bacterias halófilas entre Bacillus, Halobacillus y Virgibacillus, señalaron baja sensibilidad a gentamicina y resistencia a rifampicina y tetraciclina, como se obtuvo para las cepas BH1, BH6, BH12, BH14, BH19 y BH20, que indican multirresistencia. Por otro lado, Puig et al (2019) determinaron un patrón de multirresistencia para cloranfenicol, eritromicina y tetraciclina de Staphylococcus aislado de pescado, comprometiendo pérdidas en la producción y seguridad alimentaria.…”