Abstract:Background: Aquatic microorganisms have an important role in the bioremediation of environmental pollutants. Polycyclic Aromatic Hydrocarbons (PAHs) are described as dangerous pollutants that can bind covalently to the nucleic acids, causing mutations. Therefore, they have carcinogenic and toxic properties. Also, are involved in diseases such as asthma, lung dysfunction, and chronic bronchitis. This study aimed to isolate and characterize aquatic bio-degrading bacteria from the world’s largest lake, Khazar, wi… Show more
“…The length of fragments were approximately ≈ 1500 bp Fig. 2 Neighbor-joining phylogenetic tree [ 26 ] derived from 16S rRNA gene sequence data [ 14 ] showing the positions of seven isolated bacteria including strains Khuz-1, Khuz-2 and Khuz-3 among related bacteria. Numbers at branch points are bootstrap percentages based on 500 replicates.…”
Section: Resultsmentioning
confidence: 99%
“…Scientifics information on the safety and effectiveness of natural antibiotics’ remedies have shown that these agents have good anti-pathogenic activity with very low side effects [ 7 , 8 ]. The among microorganisms, environmental bacteria have various beneficial potations in medical and pharmaceutical applications such as bio-absorption of heavy metals [ 31 ], bio-remediation of toxics [ 14 ], production of antimicrobial peptides and antibiotics [ 32 – 34 ]. One of the most important methods for strain identification and distribution is 16S rRNA sequencing method [ 11 ].…”
Section: Discussionmentioning
confidence: 99%
“…Genomic DNA extraction was done by DNA extraction Kit (Cinagene DNa plus, South Korea), according to manufacturer’s protocol. Universal used primers were 16F 5’- AGAGTTTGATCCTGGCTCAG-3’, and reverse 16R; 5’- TACCTTGTTAGGACTTCACC-3’ primers [ 14 ]. Each amplification reaction contained 1µL of each primer, dNTP (10 mM) 0.5 µm, PCR buffer 2.5 µL, MgCl 2 (50 mM) 0.75 µL, template DNA 1 µL, smartaq DNA polymerase 0.2 µL, and dH 2 O 19.05 µL, in a final volume of 25µL.…”
Section: Methodsmentioning
confidence: 99%
“…In addition, the sequences were conducted in the EzBioCloud identity service led to retrieving its closest phylogenetic neighbors [ 17 ]. The 16S rRNA gene reads were assembled using Chromas pro software and aligned using the multiple sequence alignment program CLUSTAL_X (version 1.83) [ 18 ] Phylogenetic analysis was performed using the neighbor-joining method in MEGA version 7 software package [ 14 , 19 ].…”
Background
Multi-drug resistant bacteria hazards to the health of humans could be an agent in the destruction of human generation. Natural products of Bacillus species are the main source to access progressive antibiotics that can be a good candidate for the discovery of novel antibiotics. Wild honey as a valuable food has been used in medicine with antimicrobial effects.
Objective
Bacillus strains isolated from wild honey were evaluated for the potential antimicrobial activity against human and plant bacterial and fungal pathogens.
Methods
Three bacterial isolates were identified as strain Khuz-1 (98.27% similarity with Bacillus safensis subsp. Safensis strain FO-36bT), strain Khuz-2 (99.18% similarity with Bacillus rugosus strain SPB7T), and strain Khuz-3 (99.78% similarity with Bacillus velezensis strain CR-502 T) by 16S rRNA gene sequences. The strains were characterized by their ability to inhibit the growth of human and phytopathogenic fungi.
Results
The results indicated that B. rugosus strain Khuz-2 inhibited the growth of phytopathogenic and human fungal more effective than other ones. It seems that the strain Khuz-2 has a suitable antimicrobial and antifungal potential as a good candidate for further pharmaceutical research.
Conclusion
Based on the results of GC–MS, Pyrrolo [1,2-a] pyrazine-1,4-dion, hexahydro-3-(2-methylpropyle) (PPDHM) was the major compound for all strains which have a various pharmacological effect. Isolation and identification of beneficial bacteria from natural sources can play an important role in future pharmaceutical and industrial applications.
“…The length of fragments were approximately ≈ 1500 bp Fig. 2 Neighbor-joining phylogenetic tree [ 26 ] derived from 16S rRNA gene sequence data [ 14 ] showing the positions of seven isolated bacteria including strains Khuz-1, Khuz-2 and Khuz-3 among related bacteria. Numbers at branch points are bootstrap percentages based on 500 replicates.…”
Section: Resultsmentioning
confidence: 99%
“…Scientifics information on the safety and effectiveness of natural antibiotics’ remedies have shown that these agents have good anti-pathogenic activity with very low side effects [ 7 , 8 ]. The among microorganisms, environmental bacteria have various beneficial potations in medical and pharmaceutical applications such as bio-absorption of heavy metals [ 31 ], bio-remediation of toxics [ 14 ], production of antimicrobial peptides and antibiotics [ 32 – 34 ]. One of the most important methods for strain identification and distribution is 16S rRNA sequencing method [ 11 ].…”
Section: Discussionmentioning
confidence: 99%
“…Genomic DNA extraction was done by DNA extraction Kit (Cinagene DNa plus, South Korea), according to manufacturer’s protocol. Universal used primers were 16F 5’- AGAGTTTGATCCTGGCTCAG-3’, and reverse 16R; 5’- TACCTTGTTAGGACTTCACC-3’ primers [ 14 ]. Each amplification reaction contained 1µL of each primer, dNTP (10 mM) 0.5 µm, PCR buffer 2.5 µL, MgCl 2 (50 mM) 0.75 µL, template DNA 1 µL, smartaq DNA polymerase 0.2 µL, and dH 2 O 19.05 µL, in a final volume of 25µL.…”
Section: Methodsmentioning
confidence: 99%
“…In addition, the sequences were conducted in the EzBioCloud identity service led to retrieving its closest phylogenetic neighbors [ 17 ]. The 16S rRNA gene reads were assembled using Chromas pro software and aligned using the multiple sequence alignment program CLUSTAL_X (version 1.83) [ 18 ] Phylogenetic analysis was performed using the neighbor-joining method in MEGA version 7 software package [ 14 , 19 ].…”
Background
Multi-drug resistant bacteria hazards to the health of humans could be an agent in the destruction of human generation. Natural products of Bacillus species are the main source to access progressive antibiotics that can be a good candidate for the discovery of novel antibiotics. Wild honey as a valuable food has been used in medicine with antimicrobial effects.
Objective
Bacillus strains isolated from wild honey were evaluated for the potential antimicrobial activity against human and plant bacterial and fungal pathogens.
Methods
Three bacterial isolates were identified as strain Khuz-1 (98.27% similarity with Bacillus safensis subsp. Safensis strain FO-36bT), strain Khuz-2 (99.18% similarity with Bacillus rugosus strain SPB7T), and strain Khuz-3 (99.78% similarity with Bacillus velezensis strain CR-502 T) by 16S rRNA gene sequences. The strains were characterized by their ability to inhibit the growth of human and phytopathogenic fungi.
Results
The results indicated that B. rugosus strain Khuz-2 inhibited the growth of phytopathogenic and human fungal more effective than other ones. It seems that the strain Khuz-2 has a suitable antimicrobial and antifungal potential as a good candidate for further pharmaceutical research.
Conclusion
Based on the results of GC–MS, Pyrrolo [1,2-a] pyrazine-1,4-dion, hexahydro-3-(2-methylpropyle) (PPDHM) was the major compound for all strains which have a various pharmacological effect. Isolation and identification of beneficial bacteria from natural sources can play an important role in future pharmaceutical and industrial applications.
“…In recent decades, molecular approaches such as 16s rRNA gene sequencing are utilized as genetic markers for identifying the exact bacterial species and confirming the ancient methods (Ebrahimi et al 2020). In this study, after amplification and determination of the 16S rRNA gene sequence and comparing it in the NCBI GenBank database (https://www.ncbi.nlm.nih.gov/), the results showed that the isolate was closely related to the strains of E. coli, which confirmed our phenotypical findings (The GenBank accession number for the 16S rRNA gene isolate is OL798000).…”
Section: Phenotypic and Phylogenetic Identification Of The Isolatementioning
Significance and Impact of the Study: Water treatment systems in pharmaceutical industries face various complications, in most cases due to failure of disinfection strategies, microbial contamination and biofilm formation that can lead to drop production quality or loss. This work was conducted to evaluate the anti-biofilm activity of peracetic acid on biofilm formed by isolated Escherichia coli bacteria from the water system of a pharmaceutical plant, which can provide helpful information to mimic the actual efficacy of peracetic acid in industrial water systems. A valid disinfection process using suitable disinfectants and intervals is necessary to avoid biofilm formation.
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