Isolation and identification of a novel anti-diabetic compound from Euclea undulata Thunb

Deutschländer, M.S.; Lall, Namrita; Venter, Maryna van de

doi:10.1016/j.sajb.2010.02.018

Cited by 5 publications

(7 citation statements)

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“…However, the majority of these traditional plants have not been scientifically validated for their efficacy in the treatment of diabetes. B. elliptica is among such plants traditionally used among local healers in the Eastern Cape province of South Africa [ 4 ]. Yet no scientific research has confirmed the efficacy of this plant with regard to diabetes mellitus.…”

Section: Introductionmentioning

confidence: 99%

In VitroAntidiabetic Activity and Mechanism of Action ofBrachylaena elliptica(Thunb.) DC

Sagbo

Venter

Koekemoer

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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In South Africa, the number of people suffering from diabetes is believed to be rising steadily and the current antidiabetic therapies are frequently reported to have adverse side effects. Ethnomedicinal plant use has shown promise for the development of cheaper, cost-effective antidiabetic agents with fewer side effects. The aim of this study was to investigate the antidiabetic activity and mechanism of action of aqueous leaf extract prepared from Brachylaena elliptica. The potential of the extract for cytotoxicity was evaluated using MTT assay in HepG2 cells. The effects of the plant extract on glucose utilization in HepG2 cells and L6 myotubes, triglyceride accumulation in 3T3-L1, INS-1 proliferation, glucose metabolism in INS-1 cells, and NO production in RAW macrophages were also investigated using cell culture procedures. The inhibitory effects of the extract on the activities of different enzymes including alpha-amylase, alpha-glucosidase, pancreatic lipase, dipeptidyl peptidase IV (DPP-IV), collagenase, and CYP3A4 enzymes were evaluated. The extract also tested against protein glycation using standard published procedure. The plant extract displayed low level of toxicity, where both concentrations tested did not induce 50% cell death. The extract caused a significant increase in glucose uptake in HepG2 liver cells, with efficacy significantly higher than the positive control, berberine. The crude extract also displayed no significant effect on muscle glucose uptake, triglyceride accumulation in 3T3-L1, glucose metabolism in INS-1 cells, alpha-amylase, alpha-glucosidase, DPP-IV, lipase, protein glycation, and collagenase compared to the respective positive controls. The extract displayed a proliferative effect on INS-1 cells at 25 μg/ml when compared to the negative control. The plant also produced a concentration-dependent reduction in NO production in RAW macrophages and also demonstrated weak significant inhibition on CYP3A4 activity. The findings provide evidence that B. elliptica possess antidiabetic activity and appear to exact its hypoglycemic effect independent of insulin.

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Section: Introductionmentioning

confidence: 99%

In VitroAntidiabetic Activity and Mechanism of Action ofBrachylaena elliptica(Thunb.) DC

Sagbo

Venter

Koekemoer

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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“…Leaf decoction taken orally [10,23,[32][33][34][35][36][37][38][39][40][41][42][43] Gastro-intestinal problems (biliousness)…”

Section: Diabetesmentioning

confidence: 99%

“…Leaf infusion taken orally [10,12,44] Stomach ache Roots used as substitutes for those of Gymnanthemum corymbosum [10,24,27,29] Hysteria Roots used as substitutes for those of Gymnanthemum corymbosum [10,[24][25][26] Mouthwash Leaf decoction taken orally [23,32,33,35,36,38,42,45] Pain Root infusion taken orally [10,44] Respiratory problems (pneumonia and sore throat)…”

Section: Diabetesmentioning

confidence: 99%

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Brachylaena elliptica and B. ilicifolia (Asteraceae): A Comparative Analysis of their Ethnomedicinal Uses, Phytochemistry and Biological Activities

Maroyi

2020

J. Pharm. Nutr. Sci.

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Brachylaena elliptica and B. ilicifolia are shrubs or small trees widely used as traditional medicines in southern Africa. There is need to evaluate the existence of any correlation between the medicinal uses, phytochemistry and pharmacological properties of the two species. Therefore, in this review, analyses of the ethnomedicinal uses, phytochemistry and biological activities of B. elliptica and B. ilicifolia are presented. Results of the current study are based on data derived from several online databases such as Scopus, Google Scholar, PubMed and Science Direct, and pre-electronic sources such as scientific publications, books, dissertations, book chapters and journal articles. The articles published between 1941 and 2020 were used in this study. The leaves and roots of B. elliptica and B. ilicifolia are mainly used as a mouthwash and ethnoveterinary medicines, and traditional medicines for backache, hysteria, ulcers of the mouth, diabetes, gastro-intestinal and respiratory problems. This study showed that sesquiterpene lactones, alkaloids, essential oils, flavonoids, flavonols, phenols, proanthocyanidins, saponins and tannins have been identified from aerial parts and leaves of B. elliptica and B. ilicifolia. The leaf extracts and compounds isolated from the species exhibited antibacterial, antidiabetic, antioxidant and cytotoxicity activities. There is a need for extensive phytochemical, pharmacological and toxicological studies of crude extracts and compounds isolated from B. elliptica and B. ilicifolia.

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“…Decoctions of the leaves and roots are applied externally on boils, glandular swellings and sores to promote healing and pain relief125 . In South Africa, a tea is prepared from the leaves of the plant combined with powdered material from the Viscum species, to treat DM129 .…”

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confidence: 99%

Inhibitory potentials and kinetics of the inhibition of carbohydrate-hydrolysing enzymes by the pod and seed extracts of Lessertia montana (Fabaceae) E. Phillips & R.A. Dyer

2019

J App Pharm Sci

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Isolation and identification of a novel anti-diabetic compound from Euclea undulata Thunb

Cited by 5 publications

References 0 publications

In VitroAntidiabetic Activity and Mechanism of Action ofBrachylaena elliptica(Thunb.) DC

In VitroAntidiabetic Activity and Mechanism of Action ofBrachylaena elliptica(Thunb.) DC

Brachylaena elliptica and B. ilicifolia (Asteraceae): A Comparative Analysis of their Ethnomedicinal Uses, Phytochemistry and Biological Activities

Inhibitory potentials and kinetics of the inhibition of carbohydrate-hydrolysing enzymes by the pod and seed extracts of Lessertia montana (Fabaceae) E. Phillips & R.A. Dyer

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