Isolation and identification of a novel strain of the genus Ochrobactrum with phenol-degrading activity

El‐Sayed, Wael S.; Ibrahim, Mohamed K.; Abu-Shady, M. R.; El-Beih, Fawkia M.; Ohmura, Naoya; Saiki, H; Andõ, Akira

doi:10.1016/s1389-1723(03)80200-1

Cited by 49 publications

(8 citation statements)

References 12 publications

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“…Ochrobactrum sp. POC9 possesses complete gene repertoires for the following pathways: (i) phenol degradation through benzoate utilization [ 23 ], (ii) propanoate utilization [ 68 ], (iii) denitrification [ 69 ], and (iv) assimilatory sulfate reduction [ 70 ] ( Figure S2 ). Given the potential application of Ochrobactrum sp.…”

Section: Resultsmentioning

confidence: 99%

“…seem to be well adapted for living in contaminated environments, which are rich in xenobiotics, organic pollutants and heavy metals. Ochrobactrum strains produce a variety of hydrolyzing enzymes that enable them to utilize even hardly-degradable compounds, e.g., phenols, organophosphorus pesticides and petroleum hydrocarbons [ 22 , 23 , 24 , 25 , 26 ]. Therefore, bacteria of this genus gained attention as promising candidates for use in diverse fields of biotechnology.…”

Section: Introductionmentioning

confidence: 99%

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Genome-Guided Characterization of Ochrobactrum sp. POC9 Enhancing Sewage Sludge Utilization—Biotechnological Potential and Biosafety Considerations

Poszytek

Karczewska-Golec

Ciok

et al. 2018

IJERPH

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Sewage sludge is an abundant source of microorganisms that are metabolically active against numerous contaminants, and thus possibly useful in environmental biotechnologies. However, amongst the sewage sludge isolates, pathogenic bacteria can potentially be found, and such isolates should therefore be carefully tested before their application. A novel bacterial strain, Ochrobactrum sp. POC9, was isolated from a sewage sludge sample collected from a wastewater treatment plant. The strain exhibited lipolytic, proteolytic, cellulolytic, and amylolytic activities, which supports its application in biodegradation of complex organic compounds. We demonstrated that bioaugmentation with this strain substantially improved the overall biogas production and methane content during anaerobic digestion of sewage sludge. The POC9 genome content analysis provided a deeper insight into the biotechnological potential of this bacterium and revealed that it is a metalotolerant and a biofilm-producing strain capable of utilizing various toxic compounds. The strain is resistant to rifampicin, chloramphenicol and β-lactams. The corresponding antibiotic resistance genes (including blaOCH and cmlA/floR) were identified in the POC9 genome. Nevertheless, as only few genes in the POC9 genome might be linked to pathogenicity, and none of those genes is a critical virulence factor found in severe pathogens, the strain appears safe for application in environmental biotechnologies.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genome-Guided Characterization of Ochrobactrum sp. POC9 Enhancing Sewage Sludge Utilization—Biotechnological Potential and Biosafety Considerations

Poszytek

Karczewska-Golec

Ciok

et al. 2018

IJERPH

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“…Degradation tests for the Acinetobacter strain ATTC11171 made by Adav et al (2007) showed that this strain fully degraded 5 mM phenol, while at phenol concentrations higher than 5 mM, an inhibitory effect was observed. Phenol at low concentrations (1 and 2 mM) was degraded completely by Ochrobactrum AS1 and Fusarium flocciferum (El-Sayed et al 2003; Mendonça et al 2004). Our results show that strain KB2 exhibits ability to degrade a wide range of aromatic compounds at relatively high concentrations what makes it exceedingly attractive for industrial applications especially in bioremediation and wastewater treatment.…”

Section: Resultsmentioning

confidence: 99%

Influence of metal ions on bioremediation activity of protocatechuate 3,4-dioxygenase from Stenotrophomonas maltophilia KB2

Hupert-Kocurek

Sałek

Wojcieszyńska

2012

World J Microbiol Biotechnol

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The aim of this paper was to describe the effect of various metal ions on the activity of protocatechuate 3,4-dioxygenase from Stenotrophomonas maltophilia KB2. We also compared activity of different dioxygenases isolated from this strain, in the presence of metal ions, after induction by various aromatic compounds. S. maltophilia KB2 degraded 13 mM 3,4-dihydroxybenzoate, 10 mM benzoic acid and 12 mM phenol within 24 h of incubation. In the presence of dihydroxybenzoate and benzoate, the activity of protocatechuate 3,4-dioxygenase and catechol 1,2-dioxygenase was observed. Although Fe3+, Cu2+, Zn2+, Co2+, Al3+, Cd2+, Ni2+ and Mn2+ ions caused 20–80 % inhibition of protocatechuate 3,4-dioxygenase activity, the above-mentioned metal ions (with the exception of Ni2+) inhibited catechol 1,2-dioxygenase to a lesser extent or even activate the enzyme. Retaining activity of at least one of three dioxygenases from strain KB2 in the presence of metal ions makes it an ideal bacterium for bioremediation of contaminated areas.

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“…Several Ochrobactrum spp. have been investigated for their potential to degrade xenobiotic pollutants and for heavy metal detoxification under a variety of environmental conditions [ 13 , 14 , 15 , 16 ]. Ochrobactrum spp.…”

Section: Introductionmentioning

confidence: 99%

The Genus Ochrobactrum as Major Opportunistic Pathogens

Ryan

Pembroke

2020

Microorganisms

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Ochrobactrum species are non-enteric, Gram-negative organisms that are closely related to the genus Brucella. Since the designation of the genus in 1988, several distinct species have now been characterised and implicated as opportunistic pathogens in multiple outbreaks. Here, we examine the genus, its members, diagnostic tools used for identification, data from recent Ochrobactrum whole genome sequencing and the pathogenicity associated with reported Ochrobactrum infections. This review identified 128 instances of Ochrobactrum spp. infections that have been discussed in the literature. These findings indicate that infection review programs should consider investigation of possible Ochrobactrum spp. outbreaks if these bacteria are clinically isolated in more than one patient and that Ochrobactrum spp. are more important pathogens than previously thought.

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Isolation and identification of a novel strain of the genus Ochrobactrum with phenol-degrading activity

Cited by 49 publications

References 12 publications

Genome-Guided Characterization of Ochrobactrum sp. POC9 Enhancing Sewage Sludge Utilization—Biotechnological Potential and Biosafety Considerations

Genome-Guided Characterization of Ochrobactrum sp. POC9 Enhancing Sewage Sludge Utilization—Biotechnological Potential and Biosafety Considerations

Influence of metal ions on bioremediation activity of protocatechuate 3,4-dioxygenase from Stenotrophomonas maltophilia KB2

The Genus Ochrobactrum as Major Opportunistic Pathogens

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