Isolation and Growth of Rat Cytomegalovirus in Vitro

Rabson, Alan S.; Edgcomb, John H.; Legallais, Frances Y.; Tyrrell, Sandra A.

doi:10.3181/00379727-131-34010

Cited by 14 publications

(11 citation statements)

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“…Experimental studies in rats, however, have seldom been reported although CMV-]ikc viruses isolated from rats have been described. ]~ABSO~ et al (16) …”

Section: Introductionmentioning

confidence: 99%

Isolation of a cytomegalovirus-like agent from wild rats

Bruggeman¹,

Meijer²,

Dormans³

et al. 1982

Archives of Virology

109

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In 8 of 10 wild rats trapped in The Netherlands, an infectious viruslike agent was isolated predominantly from the salivary glands and could be serially passed in laboratory rats. In rat embryo cells a typical cytomegalo-like cytopathic effect was produced. The morphologic and cultural characteristics of the isolated agent were comparable with those of the mouse cytomegalovirus (MCMV). The virus-nucleocapsid had a size of 92 nm and was not ether-resistant. The extracellular nucleocapsids were often enclosed by an outer layer of very variable shape and size. The formation of Fc receptors on cells infected with the rat virus could be demonstrated. The wild rats possessed neutralizing antibodies to the isolated agent. The rat agent grew only in rat embryo fibroblast cells while MCMV grew in rat and mouse embryo cells. The rat agent gave plaques in REF monolayers. Electron microscope studies showed the presence of nucleocapsids in the nucleus.

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“…Experimental studies in rats, however, have seldom been reported although CMV-]ikc viruses isolated from rats have been described. ]~ABSO~ et al (16) …”

Section: Introductionmentioning

confidence: 99%

Isolation of a cytomegalovirus-like agent from wild rats

Bruggeman¹,

Meijer²,

Dormans³

et al. 1982

Archives of Virology

109

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“…In other cases, the animal in questionwas used for experimental infection of hCMV (Kuttner and Wang, 1934). More recently, a nurober of agents were discovered by exarnining animal tissue used for cell cultures (Rabson et al, 1969).…”

Section: History Of Nonhuman Cytomegalovirusesmentioning

confidence: 99%

Cytomegalovirus

1991

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All rights reserved 90-14345 CIP No part of this book may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, microfilming, recording, or otherwise, without written permission from the Publisher Preface to the First EditionAlthough there are a number of excellent current reviews on one or another aspect of cytomegalovirus, the last comprehensive treatment of this subject was that of Krech et al. (197la). In view of the amazing advances in the virological, epidemiologic, and clinical knowledge of cytomegaloviruses, an up-to-date book is needed. Such a work should cover many areas of expertise and a voluminous technical literature. Each area might have been reviewed and analyzed by workers more expert than myself. However, I have embarked on the entire venture alone in order to attain unity and continuity in this book, characteristics that are not easily achieved in the more popular multiauthored works. I have tried to review the Iiterature and provide a critical summary for each area discussed. To do this, I provide as much of the primary data of the relevant works as needed and not just the qualitative conclusions. Inevitably, the flow of the narrative may be interrupted by dry facts and figures. However, such information is essential to make this a meaningful reference work. But for those not interested in such details, I have provided at what I hope are crucial points critiques and summaries.This book is not an exhaustive review of all the literature. This is probably no Ionger possible or even desirable. By selection, however, one runs the risk of having missed or ignored important papers. I am keenly aware of this, and I wish to apologize for such oversight, if that is possible.I visualize this work as being useful for physicians, scientists, and students interested in the biology of and infection by human cytomegalovirus. I feel that to understand these, the basic virology of this virus and related herpesviruses must be included. Topics that have not advanced a great deal and that are adequately described in available works, such as the anatomic pathology, have not been reviewed in detail. The emphasis has been placed on newer insights in the virology, immunology, serology, epidemiology, and especially clinical aspects of human cytomegalovirus. I wish tobring out the interactions of knowledge in these areas. Part II is a much shorter treatment of the nonhuman cytomegaloviruses, reviewed primarily from the point of view of their contribution to the understanding of the human virus. V vi Preface to the First Edition Finally, I wish to thank Drs. Charles R. Rinaldo, Jr., John A. Armstrong, and Donald N. Medearis for their critical review of the manuscript. Their suggestions were invaluable. Dr. John N. Dowling's thorough review of CMV infection in patients with malignancies was the basis of Section 13.4. I also acknowledge the indispensable and patient secretarial assistance of Ms.The remarkable medical events and advances in knowle...

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“…This line contains no EB particles by electron microscopy. I n HSV experiments, the following cells were used: a cell line derived from LBN rat kidney (9) ; secondary cultures of newborn Sprague-Dawley (SD) rat kidneys prepared by trypsinization ; primary human embryo kidney cells, and the Vero line of African green monkey kidney cells. Virus pools of HSV were prepared in HEp-2 human epidermoid carcinoma cells.…”

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confidence: 99%