Isolation and functional analysis of CONSTANS-LIKE genes suggests that a central role for CONSTANS in flowering time control is not evolutionarily conserved in Medicago truncatula

Wong, Albert Chern Sun; Hecht, ValÃ©rie F. G.; Picard, Kelsey L.; Diwadkar, Payal; Laurie, Rebecca E.; Wen, Jiangqi; Mysore, Kirankumar S.; Macknight, Richard C.; Weller, James L.

doi:10.3389/fpls.2014.00486

Cited by 88 publications

(115 citation statements)

References 63 publications

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“…In both monocots and dicots the COL (CONSTANS-Like) family has many members such as 17 in Arabidopsis, 16 in rice, 9 in barley (Griffiths et al 2003), 10 in sugarbeet (Chia et al 2008), 11 in Medicago (Wong et al 2014), 26 in soybean (Wu et al 2014) and 23 in cotton (Zhang et al 2015). All of these COL genes are characterized by the presence of three domains namely the B-box 1, B-box 2 and a CCT (CO, COlike, and TOC1) domain (Griffiths et al 2003;Putterill et al 1995;Strayer et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of CONSTANS-Like (COL) genes in banana (Musa acuminata L. AAA Group, cv. Grand Nain)

Chaurasia

Patil²,

Azeez³

et al. 2016

Physiol Mol Biol Plants

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The CONSTANS (CO) family is an important regulator of flowering in photoperiod sensitive plants. But information regarding their role in day neutral plants is limited. We report identification of nine Group I type CONSTANS-like (COL) genes of banana and their characterization for their age dependent, diurnal and tissue-specific expression. Our studies show that the Group I genes are conserved in structure to members in other plants. Expression of these genes shows a distinct circadian regulation with a peak during light period. Developmental stage specific expression reveals high level transcript accumulation of two genes, MaCOL3a and MaCOL3b, well before flowering and until the initiation of flowering. A decrease in their transcript levels after initiation of flowering is followed by an increase in transcription of other members that coincides with the continued development of the inflorescence and fruiting. CO binding cis-elements are observed in at least three FT-like genes in banana suggesting possible CO-FT interactions that might regulate flowering. Distinct tissue specific expression patterns are observed for different family members in mature leaves, apical inflorescence, bracts, fruit skin and fruit pulp suggesting possible roles other than flowering. This is the first exhaustive study of the COL genes belonging to Group I of banana.

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Section: Introductionmentioning

confidence: 99%

Molecular characterization of CONSTANS-Like (COL) genes in banana (Musa acuminata L. AAA Group, cv. Grand Nain)

Chaurasia

Patil²,

Azeez³

et al. 2016

Physiol Mol Biol Plants

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“…Consistent with several other CO-like genes in arabidopsis (COL1 and COL2) (Ledger et al, 2001), pisum sativum (pea) (PsCOLa) (Hecht et al, 2007), and medicago (COLs) (Wong et al, 2014), PpCOL-1 showed a morning phase expression rhythm where its expression peak during morning and fall towards afternoon. This is different than the expression of CO in arabidopsis, which are regulated by light and peaked at the end of the day (Suarez-Lopez et al, 2001).…”

Section: Isolation Of Ppcol-1 and Ppphyb In Pongamiasupporting

confidence: 77%

“…PpCOL-1 showed a morning phase expression rhythm, which is similar to the CO-like in other species under the LD condition; COL1 and COL2 in Arabidopsis (Ledger et al, 2001), PsCOLa in pea (Hecht et al, 2007), and also several other COLs in Medicago (Wong et al, 2014). Their expression levels peak during the morning, then decline during the afternoon.…”

Section: Transcriptional Repressor Cycling Dof Factor (Cdf) (Fornarasupporting

confidence: 59%

“…In these studies, overexpression of the COLs was able to restore the late flowering phenotype of co mutants in arabidopsis. However, the same result was not observed in the overexpression of medicago COLs in the late flowering mutant of arabidopsis, and rule out the role of COLs as a central integrator of photoperiod response of flowering in medicago (Wong et al, 2014). Similar ectopic overexpression studies can also be done…”

Section: Limitations and Future Directionssupporting

confidence: 52%

“…In legumes such as Pisum sativum (pea), Glycine max (Soybean) and Medicago truncatula (Medicago), studies on flowering gene orthologues have discovered several genes with a conserved role in the regulation of flowering time in response to photoperiod. However, the function of these CO homologues in photoperiod response is still not clear (Hecht et al, 2005;Hecht et al, 2007;Jiang et al, 2011;Liu et al, 2011;Watanabe et al, 2011;Weller et al, 2012;Wong et al, 2014).…”

Section: Timing Of Cab Expression (Cct)mentioning

confidence: 99%

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Molecular characterisation of flowering genes in Pongamia Pinnata

Kamde¹

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Pongamia pinnata (Pongamia) is a multipurpose tree that has been used traditionally for medicine, green manure, insecticide, fuel and also a source of timber. It is an indigenous plant to the Indian subcontinent and Southeast Asia, and has been successfully introduced to Northern Australia, New Zealand and several other countries with humid tropical lowlands. Recently, Pongamia has attracted interest as one of the alternative feedstock for biodiesel productions due to its ability to produce seeds with high oil content. The fatty acid methyl ester (FAME) composition of Pongamia met the biodiesel specification standards of USA (ASTMD 6751), Germany (DIN 51606) and European Standard Organisation (EN 14214), and is considered as one of the most suitable sources of biodiesel. Despite the positive attributes described above, Pongamia is not a domesticated species and further research is required for the selection of elite cultivars with desirable traits optimal for large scale production of Pongamia oil. In addition, limited information is currently available regarding the flowering mechanism of Pongamia at the molecular level that could provide the platform for the development of oil-rich seeds.The aim of this thesis is to provide insight and increase the understanding of the genetic network that leads to flowering in Pongamia. Based on large phenotypic diversity of Pongamia, this information could subsequently be used to determine and select traits with unique flowering time that will allow a harvesting window, where harvesting process will not clash with flowering. The first objective of this project is to identify, isolate and characterise the candidate genes that regulate flowering time in Pongamia. This was achieved using molecular biology techniques and bioinformatic approaches based on the information available on flowering mechanisms of Arabidopsis and soybean in the literature. The second objective of this project is to determine the expression pattern of isolated genes during flowering onset. This was achieved through transcriptome methodologies such as RT-PCR and qRT-PCR. The third objective of this project is to investigate the genetic variety of Pongamia. This was achieved through Single Nucleotide Polymorphism (SNPs) analysis and its correlation with the late flowering phenotype of Pongamia.PpCOL-1 and PpPHYB are the two genes that were investigated in this thesis. The orthologue genes of PpCOL-1 and PpPHYB are involved in the flowering regulation of the annual plant Arabidopsis.Since Arabidopsis and Pongamia are both long day plants, it is postulated that these genes might play a role in the flowering time control in Pongamia. PpCOL-1 and PpPHYB were successfully isolated, characterised and compared to their respective orthologue genes in Arabidopsis, Soybean and Medicago. It was found that the amino acid residues within the domain regions in these genes iii are highly conserved in Pongamia. Interestingly, there were no significant differences in the expression patterns of PpCOL-1 and PpPHYB in...

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Genetic control of flowering time in legumes

Weller¹,

Macknight²

2019

The Model Legume Medicago Truncatula

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The timing of flowering, and in particular the degree to which it is responsive to the environment, is a key factor in the adaptation of a given species to various ecogeographic locations and agricultural practices. Flowering time variation has been documented in many crop legumes, and selection for specific variants has permitted significant expansion and improvement in cultivation, from prehistoric times to the present day. Recent advances in legume genomics have accelerated the process of gene identification and functional analysis, and opened up new prospects for a molecular understanding of flowering time adaptation in this important crop group. Within the legumes, two species have been prominent in flowering time studies; the vernalization-responsive long-day species pea (Pisum sativum) and the warm-season short-day plant soybean (Glycine max). Analysis of flowering in these species is now being complemented by reverse genetics capabilities in the model legumes Medicago truncatula and Lotus japonicus, and the emergence of genome-scale resources in a range of other legumes. This review will outline the insights gained from detailed forward genetic analysis of flowering time in pea and soybean, highlighting the importance of light perception, the circadian clock and the FT family of flowering integrators. It discusses the current state of knowledge on genetic mechanisms for photoperiod and vernalization response, and concludes with a broader discussion of flowering time adaptation across legumes generally.

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Isolation and functional analysis of CONSTANS-LIKE genes suggests that a central role for CONSTANS in flowering time control is not evolutionarily conserved in Medicago truncatula

Cited by 88 publications

References 63 publications

Molecular characterization of CONSTANS-Like (COL) genes in banana (Musa acuminata L. AAA Group, cv. Grand Nain)

Molecular characterization of CONSTANS-Like (COL) genes in banana (Musa acuminata L. AAA Group, cv. Grand Nain)

Molecular characterisation of flowering genes in Pongamia Pinnata

Genetic control of flowering time in legumes

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