Molecular characterization of CONSTANS-Like (COL) genes in banana (Musa acuminata L. AAA Group, cv. Grand Nain)

Chaurasia, Akhilesh K.; Patil, H. B.; Azeez, Abdul; Subramaniam, V. R.; Krishna, Bal; Sane, Aniruddha P.; Sane, P. V.

doi:10.1007/s12298-016-0345-3

Cited by 35 publications

(29 citation statements)

References 45 publications

(72 reference statements)

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“…Many of the genes which have been identified as potential regulators of chilling-induced ripening in pear fruit represent members of diverse gene families. Furthermore, several studies have previously indicated the diversification and neofunctionalization of VRN, VIN, CONSTANS-like gene families among others, in a multitude of plant tissues, including roots, shoots, leaves, apical meristems, buds, and flowers [67,73]. Here we provide evidence suggesting that members of these gene families have diversified to play similar roles in chillingdependent fruit ripening.…”

Section: Resultssupporting

confidence: 56%

“…In addition to FRIGIDA 4, overexpression of CONSTANS-like 9 has been shown to delay flowering and regulate the circadian clock by repressing CONSTANS and Flowering Locus T (FT) gene expression in photoperiod sensitive plants, like Arabidopsis [66]. Recently, however, CONSTANS-like gene families have been shown to display distinct, tissue-specific patterns of expression in banana fruit and pulp, in addition to other tissues [67], suggesting that CONSTANS-like gene family members play a role not only in flower development, but also, fruit development and senescence. CONSTANS-like 9, 5, 6, 13, and 14 were all differentially expressed.…”

Section: Cold and Temperature Stress-induced Processesmentioning

confidence: 99%

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Evidence for the Involvement of Vernalization-related Genes in the Regulation of Cold-induced Ripening in ‘D’Anjou’ and ‘Bartlett’ Pear Fruit

Hewitt

Hendrickson

Dhingra

2019

Preprint

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European pear (Pyrus communis L.) cultivars require a genetically pre-determined duration of cold-temperature exposure to induce autocatalytic system 2 ethylene biosynthesis and subsequent fruit ripening. The physiological responses of pear to cold-temperature-induced ripening have been well characterized, but the molecular mechanisms underlying this phenomenon continue to be elucidated. This study employed established cold temperature conditioning treatments for ripening of two pear cultivars, 'D'Anjou' and 'Bartlett'. Using a time-course transcriptomics approach, global gene expression responses of each cultivar were assessed at four different developmental stages during the cold conditioning process. Differential expression, functional annotation, and gene ontology enrichment analyses were performed. Interestingly, evidence for the involvement of cold-induced, vernalization-related genes and repressors of endodormancy release was found. These genes have not previously been described to play a role in fruit during the ripening transition. The resulting data provide insight into cultivar-specific mechanisms of cold-induced transcriptional regulation of ripening in European pear, as well as a unique comparative analysis of the two cultivars with very different cold conditioning requirements.For both cultivars, fruit softening accelerated once the fruit was transferred to 20°C. The rate of softening was more rapid for 'Bartlett' than 'D'Anjou'. RNAseq Assembly AnalysisRNAseq assembly generated 140077 contigs (Supplementary File 1). In the OmicsBox suite, the maSigPro R package was used to conduct time course differential expression analyses for both cultivars. 17,711 differentially expressed contigs (p<0.05) were identified for 'D'Anjou', with 7,174 of these contigs exhibiting significant linear or quadratic trends over time (R>0.8). In 'Bartlett' 31,481 contigs were identified as being differentially expressed, with 7,174 contigs exhibiting significant quadratic or linear trends over time (R>0.8) (Supplementary File 2). Similarities and differences in expression trends of contigs of interest between 'D'Anjou' and 'Bartlett', as well as expression patterns of differentially expressed contigs (DECs) associated with ethylene and phytohormone metabolism, abscisic acid metabolism, TCA cycle, respiration, were assessed. Additionally, in order to better understand the mechanisms underlying the chilling requirement for ripening in Pyrus, the expression of genes associated with: vernalization, flowering, dormancy, and other processes directly induced by cold/chilling were observed. Preclimacteric expression of AOX1 peaked during conditioning prior to onset of ripening (Figure 1), supporting the hypothesis. Additional key genes, including those that mediate vernalization and endodormancy release were also observed to be differentially expressed. Detailed analysis of RNAseq results and enriched gene ontologies related to phytohormone metabolism and coldresponse pathways are discussed in detail in the following sections.

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Section: Resultssupporting

confidence: 56%

Section: Cold and Temperature Stress-induced Processesmentioning

confidence: 99%

Evidence for the Involvement of Vernalization-related Genes in the Regulation of Cold-induced Ripening in ‘D’Anjou’ and ‘Bartlett’ Pear Fruit

Hewitt

Hendrickson

Dhingra

2019

Preprint

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“…The expression patterns of a subset of BBX genes was assessed during fruit development and compared with both the anthocyanin content and the expression of DFR , UFGT and MYB10 . This showed that BBX genes have a range of expression patterns, some of which increased with anthocyanin accumulation in fruit skin, similar to banana BBX expression during fruit development 80 . A number of these genes showed an expression patterns correlating with anthocyanin induction, including in particular BBX1 , 15 , 33 , 51 and 54.…”

Section: Discussionmentioning

confidence: 74%

Apple B-box factors regulate light-responsive anthocyanin biosynthesis genes

Plunkett

Henry-Kirk

Friend³

et al. 2019

Sci Rep

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Environmentally-responsive genes can affect fruit red colour via the activation of MYB transcription factors. The apple B-box (BBX) gene, BBX33/CONSTANS-like 11 (COL11) has been reported to influence apple red-skin colour in a light- and temperature-dependent manner. To further understand the role of apple BBX genes, other members of the BBX family were examined for effects on colour regulation. Expression of 23 BBX genes in apple skin was analysed during fruit development. We investigated the diurnal rhythm of expression of the BBX genes, the anthocyanin biosynthetic genes and a MYB activator, MYB10. Transactivation assays on the MYB10 promoter, showed that BBX proteins 1, 17, 15, 35, 51, and 54 were able to directly function as activators. Using truncated versions of the MYB10 promoter, a key region was identified for activation by BBX1. BBX1 enhanced the activation of MYB10 and MdbHLH3 on the promoter of the anthocyanin biosynthetic gene DFR. In transformed apple lines, over-expression of BBX1 reduced internal ethylene content and altered both cyanidin concentration and associated gene expression. We propose that, along with environmental signals, the control of MYB10 expression by BBXs in ‘Royal Gala’ fruit involves the integration of the expression of multiple BBXs to regulate fruit colour.

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“…Many of the genes that have been identified as potential regulators of cold-induced ripening in pear fruit represent members of diverse gene families. Furthermore, several studies have previously indicated the diversification and neofunctionalization of VRN, VIN, CONSTANS-like gene families among others, in a multitude of plant tissues, including roots, shoots, leaves, apical meristems, buds, and flowers 67,73 . Here we provide evidence suggesting that members of these gene families have diversified to play similar roles in cold-dependent fruit ripening.…”

Section: Resultsmentioning

confidence: 99%

“…In addition to FRIGIDA 4, overexpression of CONSTANS-like 9 has been shown to delay flowering and regulate the circadian clock by repressing CONSTANS and Flowering Locus T (FT) gene expression in photoperiod sensitive plants, like Arabidopsis 66 . Recently, however, CONSTANS-like gene families have been shown to display distinct, tissue-specific patterns of expression in banana fruit and pulp, in addition to other tissues 67 , suggesting that CONSTANS-like gene family members play a role not only in flower development, but also, fruit development and senescence. CONSTANS-like 9, 5, 6, 13, and 14 were all differentially expressed, displaying a decreasing expression trend in both cultivars throughout the conditioning period (Fig.…”

Section: Abscisic Acid (Aba) Aba Is Well-established As a Key Regulamentioning

confidence: 99%

Evidence for the Involvement of Vernalization-related Genes in the Regulation of Cold-induced Ripening in ‘D’Anjou’ and ‘Bartlett’ Pear Fruit

Hewitt

Hendrickson

Dhingra

2020

Sci Rep

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Physiological studies have characterized the conditioning requirements for a range of European pear cultivars under defined conditioning temperatures, exogenous ethylene application regimes, and other pre-harvest treatments 8-11. While exogenous ethylene treatment reduces cold conditioning needs, in most European pear cultivars it does not entirely supplant the need for cold conditioning. This indicates that cold-dependent mechanisms are partly responsible for regulating the development of ripening competency, which in turn impacts the quality and marketability of pear fruit. Interestingly, in contrast to P. communis and P. bretschneideri, many Japanese pear (Pyrus pyrifolia L.) varieties have no conditioning requirements and are also regarded as non-climacteric fruits because they do not display the characteristic S1 to S2 transition during ripening 12. Additionally, 'Bosc' , unique from other European pear cultivars, acquires competency for ripening with exogenous ethylene only, needing no chilling to ripen 8. Requirement of cold exposure in pear to induce ripening is reminiscent of other natural cold temperature-dependent developmental phenomena, such as the vernalization and stratification that are needed for flowering and seed germination, respectively. The genes that regulate vernalization and stratification have been well-studied in model organisms, including Arabidopsis, wheat, and barley 13-15 ; however, similar gene homologues have not yet been reported in cold-induced fruit ripening. With respect to flowering, the process of developmental initiation following an environmentally governed dormant state is known as endodormancy release 16. Thus far, endodormancy release has not been used to characterize ripening after chilling, although the two processes share many similarities with regards to the timing and environmental nature of cold required, suggesting that similar genetic and regulatory mechanisms may govern these processes. Various forms of the chilling requirement for ripening have been described in avocado and mango, although to a lesser degree than in pear, as the former are more prone to chilling injury 17,18. Few recent studies have utilized a transcriptomics approach to characterize the molecular underpinnings of cold-induced S1 to S2 transition in pear. A complex interaction of genes is involved in regulating phytohormones, secondary messengers, signaling pathways, respiration and chromatin modification that underlies cold-induced progression of ripening 19-23. Genes associated with phytohormones such as abscisic acid (ABA), auxin, and jasmonic acid, along with several transcription factors were implicated in low-temperature-mediated enhancement of ripening in 'Bartlett' 21. In 'Passe Crassane, ' the impact of low temperature-induced ethylene (LT) and the effects of exogenous ethylene treatments were evaluated using a transcriptomics approach 24. It was observed that the expression of a subset of the LT-induced differentially expressed genes was disrupted by 1-MCP treatment, indicating that ...

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Molecular characterization of CONSTANS-Like (COL) genes in banana (Musa acuminata L. AAA Group, cv. Grand Nain)

Cited by 35 publications

References 45 publications

Evidence for the Involvement of Vernalization-related Genes in the Regulation of Cold-induced Ripening in ‘D’Anjou’ and ‘Bartlett’ Pear Fruit

Evidence for the Involvement of Vernalization-related Genes in the Regulation of Cold-induced Ripening in ‘D’Anjou’ and ‘Bartlett’ Pear Fruit

Apple B-box factors regulate light-responsive anthocyanin biosynthesis genes

Evidence for the Involvement of Vernalization-related Genes in the Regulation of Cold-induced Ripening in ‘D’Anjou’ and ‘Bartlett’ Pear Fruit

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