Isolation and functional analysis of two CONSTANS-like 1 genes from mango

Guo, Yihang; Luo, Cong; Liu, Yuan; Liang, Rongzhen; H, Yu; Lu, Xin-Xi; Mo, Xiao; Chen, Shuquan; He, Xinhua

doi:10.1016/j.plaphy.2022.01.010

Cited by 15 publications

(10 citation statements)

References 53 publications

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“…In addition, the expression levels of salt- and drought-related genes in the transgenic lines were all significantly higher than those in the WT under stress treatment, further proving that overexpression of the MiCOL9A and MiCOL9B genes can improve the resistance of Arabidopsis to salt and drought stresses. The results are also similar to those of MiCOL1A , MiCOL1B , MiCOL16A and MiCOL16B in previous research ( Guo et al., 2022 ; Liu et al., 2022 ).…”

Section: Discussionsupporting

confidence: 91%

“…Among these genes, AtCOL9 has been confirmed to be located in the nucleus, and VviCOL9a , VviCOL9b and OsCOL9 have all been confirmed to have transcriptional activation activity ( Cheng and Wang, 2005 ; Hao et al., 2016 ; Wang et al., 2019 ). In this experiment, the MiCOL9A and MiCOL9B genes were confirmed to be located in the nucleus and display transcriptional activation activity through the MR domain, similar to the findings of MiCOL1A , MiCOL1B , MiCOL16A and MiCOL16B in previous research ( Guo et al., 2022 ; Liu et al., 2022 ).…”

Section: Discussionsupporting

confidence: 90%

“…C9 transcripts accumulate to high levels in cauline leaves but to very low levels in flowers ( Jin et al., 2021 ). In our previous study, MiCO , MiCOL1A , MiCOL16A and MiCOL16B all showed similar expression patterns ( Liu et al., 2020 ; Guo et al., 2022 ; Liu et al., 2022 ). In contrast, four genes had high expression in flowers, similar to the VviCOL family genes, which are highly expressed in flower buds, suggesting that these genes may be involved in flowering induction ( Wang et al., 2019 ).…”

Section: Discussionmentioning

confidence: 72%

“…Similarly, the OsCOL9 gene inhibits flowering in rice under both LD and short-day (SD) conditions ( Hao et al., 2016 ). In a previous study, the MiCO , MiCOL1 and MiCOL16 genes all inhibited flowering under LD and SD conditions ( Guo et al., 2022 ; Liu et al., 2022 ). In this study, the flowering time of MiCOL9A - and MiCOL9B -transgenic lines was later than that of WT and pBI121 lines under LD conditions, and the numbers of rosette leaves in the transgenic lines were greater than those in the WT and pBI121 lines.…”

Section: Discussionmentioning

confidence: 87%

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Genome-wide identification of the mango CONSTANS (CO) family and functional analysis of two MiCOL9 genes in transgenic Arabidopsis

et al. 2022

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CONSTANS/CONSTANS-like (CO/COL) transcription factors play a vital role in the photoperiodic flowering pathway. However, the biological functions of COL genes in mango are unclear. In this study, we identified 31 COL genes from the ‘Jin Huang’ mango genome and divided them into three groups according to the specific gene structure and protein domain characteristics. These 31 MiCOL genes were heterogeneously distributed on 14 chromosomes. Expression pattern analysis showed that most MiCOL genes were mainly expressed in leaves and stems and during the floral induction period, followed by the floral differentiation period. The expression of COL genes was induced by drought and salt stress, but the expression patterns of different genes were different, which may suggest that MiCOL genes are involved in the abiotic stress response of mango. Under salt and drought conditions, two MiCOL9 genes can improve the resistance of Arabidopsis by improving the scavenging ability of ROS and proline accumulation and reducing the MDA content. Additionally, overexpression of MiCOL9 genes significantly inhibited flowering in transgenic Arabidopsis. This work provides an important foundation for understanding the biological roles of mango COL genes in plant growth, development and stress responses.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 72%

Section: Discussionmentioning

confidence: 87%

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Genome-wide identification of the mango CONSTANS (CO) family and functional analysis of two MiCOL9 genes in transgenic Arabidopsis

et al. 2022

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“…According to previous studies, 31 members of the CO family in mango plants were identified and divided into three groups and five subgroups (Liu et al, 2022b). The functions of MiCO , MiCOL1 , MiCOL2 , MiCOL9 , and MiCOL16 have been studied, and all of these genes exhibit repressed flowering and improved abiotic stress resistance in transgenic Arabidopsis plants (Liu et al, 2020; Guo et al, 2022; Liang et al, 2022; Liu et al, 2022a; Liu et al, 2022b). However, the functions of MiCOL6 , MiCOL7A and MiCOL7B , which have only one CCT domain belonging to group II, are still unclear.…”

Section: Introductionmentioning

confidence: 99%

MiCOL6, MiCOL7A and MiCOL7B isolated from mango regulate flowering and stress response in transgenic Arabidopsis

Liu,

Luo,

Lan

et al. 2024

Physiologia Plantarum

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The CONSTANS/CONSTANS‐Like (CO/COL) family has been shown to play important roles in flowering, stress tolerance, fruit development and ripening in higher plants. In this study, three COL genes, MiCOL6, MiCOL7A and MiCOL7B, which each contain only one CCT domain, were isolated from mango (Mangifera indica), and their functions were investigated. MiCOL7A and MiCOL7B were expressed mainly at 20 days after flowering (DAF), and all three genes were highly expressed during the flowering induction period. The expression levels of the three genes were affected by light conditions, but only MiCOL6 exhibited a clear circadian rhythm. Overexpression of MiCOL6 promoted earlier flowering, while overexpression of MiCOL7A or MiCOL7B delayed flowering compared to that in the control lines of Arabidopsis thaliana under long‐day (LD) and short‐day (SD) conditions. Overexpressing MiCOL6, MiCOL7A or MiCOL7B in transgenic plants increased superoxide dismutase (SOD) and proline levels, decreased malondialdehyde (MAD) levels, and improved survival under drought and salt stress. In addition, yeast two‐hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) analyses showed that the MiCOL6, MiCOL7A and MiCOL7B proteins interact with several stress‐ and flower‐related proteins. This work demonstrates the functions of MiCOL6, MiCOL7A and MiCOL7B and provides a foundation for further research on the role of mango COL genes in flowering regulation and the abiotic stress response.

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Isolation of three MiDi19-4 genes from mango, the ectopic expression of which confers early flowering and enhances stress tolerance in transgenic Arabidopsis

Zhu

et al. 2023

Planta

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Isolation and functional analysis of two CONSTANS-like 1 genes from mango

Cited by 15 publications

References 53 publications

Genome-wide identification of the mango CONSTANS (CO) family and functional analysis of two MiCOL9 genes in transgenic Arabidopsis

Genome-wide identification of the mango CONSTANS (CO) family and functional analysis of two MiCOL9 genes in transgenic Arabidopsis

MiCOL6, MiCOL7A and MiCOL7B isolated from mango regulate flowering and stress response in transgenic Arabidopsis

Isolation of three MiDi19-4 genes from mango, the ectopic expression of which confers early flowering and enhances stress tolerance in transgenic Arabidopsis

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