Isolation and expression analysis of CsCML genes in response to abiotic stresses in the tea plant (Camellia sinensis)

Abstract

Calmodulin-like (CML) proteins are a class of important Ca 2+ sensors in plants, which play vital roles in regulating plant growth and development and response to abiotic stress. Tea plant ( Camellia sinensis L.) is the most popular non-alcoholic economic beverage crop around the world. However, the potential functions of CMLs in either tea plants growth or in the response to environmental stresses are still unclear. In the present study, five CsCML genes ( CsCML 16, CsCML 18-1, CsCML 18-2, CsCML 38, and CsCML 42) were isolated from tea plant, and functionally characterized. The CsCML genes showed diverse expression patterns in leaves, roots, old stems, immature stems and flowers of tea plants. To investigate the expression changes of the genes under various abiotic stresses and ABA treatment, time-course experiments were also performed, the results indicated that the expression levels of CsCML16 , 18-2 and 42 were significantly induced under low temperature and salt condition, while CsCML38 was induced distinctly under drought stress and ABA treatment. Overall, Cs CML genes showed diverse function in tea plant under various stimuli. These results will increase our knowledge of the significance of CsCML genes in tea plant in response to abiotic stresses and hormone treatments.

Genome-wide investigation and transcriptional analysis of cytosine-5 DNA methyltransferase and DNA demethylase gene families in tea plant (Camellia sinensis) under abiotic stress and withering processing

“…With the release of tea reference genome, gene family identification, classification, and function prediction have gradually become new research hotspots Liu et al, 2019;Ma et al, 2019;Wang et al, 2018). With the extreme global climate, low temperature stress and drought stress may adversely affect the growth and development of tea plants and the quality of tea products.…”

Transcriptome analysis reveals underlying immune response mechanism of fungal (Penicillium oxalicum) disease in Gastrodia elata Bl. f. glauca S. Chow (Orchidaceae)

“…f. glauca were probably the member of transcription factor families like WRKY, GH3, TIFY/JAZ, CML, ERF, TGA. Coincidently, it has been reported that above transcription factors did be widely involved in various defense responses [58,[90][91][92][93][94][95][96][97][98][99][100][101][102]. It is reported that GH3 and CML can also regulates fruit development [103,104].…”

Transcriptome analysis reveals underlying immune response mechanism of fungal (Penicillium oxalicum) disease in Gastrodia elata Bl. f. glauca S. Chow (Orchidaceae)

“…f. glauca were probably the member of transcription factor families like WRKY, GH3, TIFY/JAZ, CML, ERF, TGA. Coincidently, it has been reported that above transcription factors did be widely involved in various defense responses [26,[66][67][68][69][70][71][72][73][74][75][76][77][78]. It is reported that GH3 and CML can also regulates fruit development [79,80].…”