Transcriptome analysis reveals underlying immune response mechanism of fungal (Penicillium oxalicum) disease in Gastrodia elata Bl. f. glauca S. Chow (Orchidaceae)

Wang, Yanhua; Gao, Yugang; Zang, Pu; Xu, Yue

doi:10.21203/rs.3.rs-29231/v2

Cited by 2 publications

(2 citation statements)

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“…The unstable compatibility between strains from other areas and G. elata Bl. f. glauca affects its yield [28]. It is vital to isolate, identify and screen symbiotic Armillaria, which is beneficial to the growth of G. elata Bl.…”

Section: Introductionmentioning

confidence: 99%

An exploration of mechanism of high quality and yield of Gastrodia elata Bl. f. glauca by the isolation, identification and evaluation of Armillaria

Gao

et al. 2022

BMC Plant Biol

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Background Gastrodia elata Bl. f. glauca, a perennial herb of G.elata Bl. in Orchidaceae, is one of the most valuable traditional Chinese medicines. G. elata Bl. is a chlorophyll-free myco-heterotrophic plant, which must rely on the symbiotic growth of Armillaria, but not all Armillaria strains can play the symbiotic role. Additionally, Armillaria is easy to degenerate after multiple generations, and the compatibility between the strains from other areas and G. elata Bl. f. glauca in Changbai Mountain is unstable. Therefore, it is incredibly significant to isolate, identify and screen the symbiotic Armillaria suitable for the growth of G. elata Bl. f. glauca in Changbai Mountain, and to explore the mechanism by which Armillaria improves the production performance of G. elata Bl. f. glauca. Results Firstly, G. elata Bl. f. glauca tubers, and rhizomorphs and fruiting bodies of Armillaria were used for the isolation and identification of Armillaria. Five Armillaria isolates were obtained in our laboratory and named: JMG, JMA, JMB, JMC and JMD. Secondly, Armillaria was selected based on the yield and the effective component content of G. elata Bl. f. glauca. It was concluded that the yield and quality of G. elata Bl. f. glauca co-planted with JMG is the highest. Finally, the mechanism of its high quality and yield was explored by investigating the effects of different Armillaria strains on the soil, its nutrition element contents and the soil microbial diversity around G. elata Bl. f. glauca in Changbai Mountain. Conclusions Compared with commercial strains, JMG significantly increased the content of Na, Al, Si, Mn, Fe, Zn, Rb and the absorption of C, Na, Mg, Ca, Cr, Cu, Zn and Rb in G. elata Bl. f. glauca; it improved the composition, diversity and metabolic functions of soil microbial communities around G. elata Bl. f. glauca at phylum, class and genus levels; it markedly increased the relative abundance of bacteria such as Chthoniobacter and Armillaria in the dominant populations, and enhanced such functions as Cell motility, amino acid metabolism and Lipid metabolism; it dramatically decreased the relative abundance of Bryobacter and other fungi in the dominant populations, and reduced such functions as microbial energy metabolism, translation and carbohydrate metabolism. This is the main reason why excellent Armillaria strains promote the high quality and yield of G. elata Bl. f. glauca in Changbai Mountain.

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Section: Introductionmentioning

confidence: 99%

An exploration of mechanism of high quality and yield of Gastrodia elata Bl. f. glauca by the isolation, identification and evaluation of Armillaria

Gao

et al. 2022

BMC Plant Biol

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“…Transcriptomics has been used to find key genes in recent years, proteins or metabolites related to resistance in plants, and findings have revealed new methods of studying the molecular mechanisms plants response to pathogen infection and resistance breeding [25]. Using comparative transcriptomics, many DEGs related to disease resistance have been identified in different species, such as gerbera [26], gastrodia [27] and cucumber [28]. In addition, transcriptomics has also been used to identify powdery mildew resistance genes in other gramineous plants, including maize [29], wheat [30] and barley [31], as well as key genes and metabolic pathways of plant responses to pathogen infection, thus providing a theoretical basis for revealing the mechanism underlying plants disease resistance response.…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptome analysis of resistant and susceptible Kentucky bluegrass varieties in response to powdery mildew infection

et al. 2022

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Background Poa pratensis is one of the most common cold-season turfgrasses used for urban turf building, and it is also widely used in ecological environment management worldwide. Powdery mildew is a common disease of P. pratensis. To scientifically and ecologically control lawn powdery mildew, the molecular mechanism underlying the response of P. pratensis to powdery mildew infection must better understood. Results To explore molecular mechanism underlying the response of P. pratensis to powdery mildew infection, this study compared physiological changes and transcriptomic level differences between the highly resistant variety ‘BlackJack’ and the extremely susceptible variety ‘EverGlade’ under powdery mildew infection conditions. We analyzed DEGs using reference canonical pathways in the Kyoto Encyclopedia of Genes and Genomes (KEGG) database, and the results showed that “starch and sucrose metabolism”, “photosynthesis” and “fatty acid metabolism”pathways were only enriched in ‘BlackJack’, and the expression of DEGs such as HXK, INV, GS, SS, AGpase and β-amylase in “starch and sucrose metabolism” pathway of ‘BlackJack’ were closely related to powdery mildew resistance. Meanwhile, compared with ‘EverGlade’, powdery mildew infection promoted synthesis of sucrose, expression of photosynthesis parameters and photosynthesis-related enzymes in leaves of ‘BlackJack’ and decreased accumulation of monosaccharides such as glucose and fructose. Conclusions This study identified the key metabolic pathways of a P. pratensis variety with high resistance to powdery mildew infection and explored the differences in physiological characteristics and key genes related to sugar metabolism pathways under powdery mildew stress. These findings provide important insights for studying underlying molecular response mechanism.

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Transcriptome analysis reveals underlying immune response mechanism of fungal (Penicillium oxalicum) disease in Gastrodia elata Bl. f. glauca S. Chow (Orchidaceae)

Cited by 2 publications

References 85 publications

An exploration of mechanism of high quality and yield of Gastrodia elata Bl. f. glauca by the isolation, identification and evaluation of Armillaria

An exploration of mechanism of high quality and yield of Gastrodia elata Bl. f. glauca by the isolation, identification and evaluation of Armillaria

Comparative transcriptome analysis of resistant and susceptible Kentucky bluegrass varieties in response to powdery mildew infection

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