Isolation and enzyme bioprospection of endophytic bacteria associated with plants of Brazilian mangrove ecosystem

Abstract

The mangrove ecosystem is a coastal tropical biome located in the transition zone between land and sea that is characterized by periodic flooding, which confers unique and specific environmental conditions on this biome. In these ecosystems, the vegetation is dominated by a particular group of plant species that provide a unique environment harboring diverse groups of microorganisms, including the endophytic microorganisms that are the focus of this study. Because of their intimate association with plants, endophytic microorganisms could be explored for biotechnologically significant products, such as enzymes, proteins, antibiotics and others. Here, we isolated endophytic microorganisms from two mangrove species, Rhizophora mangle and Avicennia nitida, that are found in streams in two mangrove systems in Bertioga and Cananéia, Brazil. Bacillus was the most frequently isolated genus, comprising 42% of the species isolated from Cananéia and 28% of the species from Bertioga. However, other common endophytic genera such as Pantoea, Curtobacterium and Enterobacter were also found. After identifying the isolates, the bacterial communities were evaluated for enzyme production. Protease activity was observed in 75% of the isolates, while endoglucanase activity occurred in 62% of the isolates. Bacillus showed the highest activity rates for amylase and esterase and endoglucanase. To our knowledge, this is the first reported diversity analysis performed on endophytic bacteria obtained from the branches of mangrove trees and the first overview of the specific enzymes produced by different bacterial genera. This work contributes to our knowledge of the microorganisms and enzymes present in mangrove ecosystems.

Isolation, diversity, and biotechnological potential of rhizo- and endophytic bacteria associated with mangrove plants from Saudi Arabia

“…A previous study reported the isolation and identification of important enzymes, such as amylase, esterase, cellulose, and protease from endophytes of mangroves (Castro et al, 2014). Further, production of exo-and endoglucanases have been reported in different groups of bacteria isolated from mangrove sediments (Soares Júnior et al, 2013).…”

Potensi Ekosistem Mangrove Sebagai Sumber Bakteri Untuk Produksi Protease, Amilase Dan Selulase

“…Tabao et al (2010) melaporkan 10 isolat bakteri penghasil selulase yang diisolasi dari berbagai lokasi mangrove di Filipina. Thompson et al (2013) juga melaporkan isolat bakteri selulolitik dari mangrove di Brazil, Castro, et al (2014) 478,2 ± 65,2 0 118,9 ± 7,4 SMT 1.7 284,2 ± 46,3 0 66,4 ± 17,2 SMT 2.5 323,5 ± 10,18 0 225,4 ± 3,7 SMT 2.6 285,2 ± 2,97 0 103,1 ± 3,3 SMT 2.7 210,0 ± 0,51 287, 1 ± 25,5 134,9 ± 4,3 SMT 3.5 291,9 ± 3,62 457,1 ± 29,5 121,4 ± 4,7 SMT 3.6 183,6 ± 14,67 333,4 ± 63,6 110,6 ± 1,2 SMT 4.5 470,9 ± 2,34 406,3 ± 3,8 935 ± 6,1 penghasil selulase dan 3 isolat bakteri penghasil amilase dari mangrove di Ratnagairi, India. Chantarasiri (2016) mengidentifikasi Bacillus cereus yang memiliki aktivitas selulase dari mangrove di Thailand.…”

Characterisation of endophytic bacteria from a desert plant Lepidium perfoliatum L.

“…To determine esterase activity, previously sterilised Tween 80 was added to the sterilised culture medium containing (in g/l) peptone 10, NaCl 5, CaCl·H 2 O 0.1, pH 7.4 to a final concentration of 1% (v/v) to evaluate the bacterial esterase activity. The presence of halos was considered to indicate enzymatic activity (Castro et al 2014). To determine protease activity, we used a culture medium containing skim milk; and the formation of a halo around the colony was considered indicative of proteolytic activity (Castro et al 2014).…”

“…The presence of halos was considered to indicate enzymatic activity (Castro et al 2014). To determine protease activity, we used a culture medium containing skim milk; and the formation of a halo around the colony was considered indicative of proteolytic activity (Castro et al 2014).…”