Characterisation of endophytic bacteria from a desert plant Lepidium perfoliatum L.

Li, Yuanting; Chen, Cong; An, Deng-Di

doi:10.17221/14/2016-pps

Cited by 17 publications

(3 citation statements)

References 37 publications

(45 reference statements)

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“…All the endophytes investigated in the present study resisted water stress at 20% PEG, with 82% of the endophytes exhibiting tolerance at 40% PEG ( Figure 2 ). Similarly, all 62 bacteria isolated from Lepidium perfoliatum [ 29 ], and 16 fungi endophytes isolated from Triticum aestivum [ 30 ] grew in media culture containing 20% PEG. However, unlike the fungi isolated from E. obtisufolius , the fungi isolated from wheat could not survive at a 40% PEG concentration [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

Growth-Promoting Characteristics of Fungal and Bacterial Endophytes Isolated from a Drought-Tolerant Mint Species Endostemon obtusifolius (E. Mey. ex Benth.) N. E. Br

Ogbe

Gupta

Stirk

et al. 2023

Plants

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Endophytes are primarily endosymbiotic bacteria and fungi that colonize the interior tissues of their host plant. They enhance the host plant’s growth and attenuate adverse effects of biological stress. Endophytic species of many indigenous plants are an untapped resource of plant growth-promoting microorganisms that can mitigate abiotic stress effects. Thus, this study aimed to isolate endophytes from the roots and leaves of the medicinal plant Endostemon obtusifolius to evaluate their in vitro growth-promoting capacities and drought tolerance and to characterize the most promising species. Twenty-six endophytes (fourteen bacteria and twelve fungi) were isolated and cultured from the roots and leaves of E. obtusifolius. All 26 endophytes produced flavonoids, and 14 strains produced phenolic compounds. Of the 11 strains that displayed good free radical scavenging capability (low IC50) in the 1-1-diphenyl-1-picryhydrazyl radical scavenging assay, only three strains could not survive the highest drought stress treatment (40% polyethylene glycol). These 11 strains were all positive for ammonia and siderophore production and only one strain failed to produce hydrogen cyanide and solubilize phosphate. Seven isolates showed aminocyclopropane-1-carboxylate deaminase activity and differentially synthesized indole-3-acetic acid. Using molecular tools, two promising symbiotic, drought stress tolerant, and plant growth-enhancing endophytic species (EORB-2 and EOLF-5) were identified as Paenibacillus polymyxa and Fusarium oxysporum. The results of this study demonstrate that P. polymyxa and F. oxysporum should be further investigated for their drought stress mitigation and plant growth enhancement effects as they have the potential to be developed for use in sustainable agricultural practices.

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Section: Discussionmentioning

confidence: 99%

Growth-Promoting Characteristics of Fungal and Bacterial Endophytes Isolated from a Drought-Tolerant Mint Species Endostemon obtusifolius (E. Mey. ex Benth.) N. E. Br

Ogbe

Gupta

Stirk

et al. 2023

Plants

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“…Interestingly, Vigani et al (2019) found that root endophytic bacteria conferred drought resistance in Capsicum annuum by modifying vacuolar H + pyrophosphatases (VPP) expression, which aided in preserving osmotic balance. Strains of Bacillus endophytes found in Lepidium perfoliatum roots were responsible for the formation of biofilm on roots, resulting in drought resistance and seedling germination (Li et al, 2017). Piriformospora indica, a wellknown root fungal endophyte, colonizes the roots of many plant species and provides multifaceted amenities, including drought alleviation (Gill et al, 2016).…”

Section: Microbiome Drivers Of Plant Fitness Benefits Under Droughtmentioning

confidence: 99%

Plant-microbiome interactions under drought—insights from the molecular machinist’s toolbox

Ait-El-Mokhtar,

Meddich,

Baslam

2023

Front. Sustain. Food Syst.

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Plants face numerous challenges in novel and harsh environments, including altered precipitation regimes, salinity, extreme temperatures, increased atmospheric CO2, nutrient deficiency, heavy metals, and oxygen. Drought remains a major constraint to crop productivity and meeting food demand, with the frequency, intensity, and duration of drought expected to raise in the coming century. The “cry for help” hypothesis proposes that timely recruiting of the microbiome by plants may confer benefits in stress alleviation, plant growth, fitness, and health. The root-associated microbiome harbors 10–100 times more functional genes than the host, which can significantly stimulate the metabolic and genetic potential of plant–microbiome assembly. However, cross-talk among drought and the root-associated microbes, and among the root-associated microbiome and the host-plant, is less well understood. Understanding the molecular aspect of multiple mechanisms by which microbes associate with plants during drought stress is of fundamental importance in plant biology and agriculture. In this review, we examine the progress in research on the response of plant and its microbiome assemblages and interactions to drought stress, including the impact of drought and root exudates on host resilience. We delve into the potential of ‘omics’ technologies to unravel the signaling networks underlying these interactions and the multiway interactions that occur among the host and its associated microbiome. We then discuss the shortfalls, challenges, and future research directions in this field. Overall, we argue that harnessing/manipulating the crop microbiome presents a promising strategy for improving agricultural systems in the face of global climate change.

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“…Many plant growth promoting bacteria have been reported to increase the drought resistance in plants like Pisum sativum, Zea mays, Vigna radiata, Triticum aestivum, Capsicum annum, Solanum tuberosum and Sorghum bicolor (Sandhya et al 2017;Yuanting et al 2017;Susilowati et al 2018;Niu et al 2018;Marulanda et al 2009). Based on these, much more candidate organisms can be well expected from stress adapted plants like A. comosus.…”

Section: Introductionmentioning

confidence: 99%

Drought tolerant bacterial endophytes with potential plant probiotic effects from Ananas comosus

Jayakumar

Padmakumar

Nair³

et al. 2020

Biologia

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Endophytic bacteria with multi-trait plant probiotic features have been demonstrated to have applications to enhance agricultural productivity by supporting the plant growth, yield and disease resistance under harsh environmental conditions. The xerophytic plant Ananas comosus was selected as the source for endophytic bacterial isolation in the current study. This has resulted in the identification of organisms with antifungal and plant growth promoting properties. Interestingly, endophytic bacteria with antifungal activity were found to have drought tolerance property also. These organisms were demonstrated to express the plant beneficial mechanisms like IAA, ACC deaminase and nitrogen fixation under drought condition. The selected organisms were further identified as Bacillus sp. (Acb9), Providencia sp. (Acb11), Staphylococcus sp. (Acb12), Staphylococcus sp. (Acb13) and Staphylococcus sp. (Acb14)(Acb 14). In addition, LC-MS/MS analysis of extract from Acb 9 has resulted in the identification of surfactin with m/z of 994. Priming of selected endophytes with Vigna radiata seedlings also showed enhanced growth parameters such as shoot length, root length and root numbers when compared to the control. The shoot length of Acb 9 treatment was found to be 18.833 ± 0.687 cm, where the control value for same was 13.976 ± 0.585 cm and the root length was 3.9 ± 0.99 when compared to control (1.54 ± 0.628). The root length was 3.9±0.99 which was also higher than control (1.54±0.62).The results of the study indicate the promise of endophytic bacteria for field application under changing agro-climatic conditions.

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Characterisation of endophytic bacteria from a desert plant Lepidium perfoliatum L.

Cited by 17 publications

References 37 publications

Growth-Promoting Characteristics of Fungal and Bacterial Endophytes Isolated from a Drought-Tolerant Mint Species Endostemon obtusifolius (E. Mey. ex Benth.) N. E. Br

Growth-Promoting Characteristics of Fungal and Bacterial Endophytes Isolated from a Drought-Tolerant Mint Species Endostemon obtusifolius (E. Mey. ex Benth.) N. E. Br

Plant-microbiome interactions under drought—insights from the molecular machinist’s toolbox

Drought tolerant bacterial endophytes with potential plant probiotic effects from Ananas comosus

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