Isolation and efficacy of the endophytic fungus, Beauveria bassiana (Bals.) Vuillemin on grapevine aphid, Aphis illinoisensis Shimer (Hemiptera: Aphididae) under laboratory conditions

Abstract

The endophytic fungus, Beauveria bassiana has been found to occur endophytically in hundreds of plant species tissues and has been inoculated for establishment in different plants. It has negative effect on piercing-sucking insects in grapevine plants. Thus, the present study aimed to detect the endophytic fungus, B. bassiana throughout ITS genes analysis in the grapevine plant, Vitis venifera cv. Taify and assess its potential for controlling the grapevine aphid, Aphis illinoisensis Shimer (Hemiptera: Aphididae). The obtained 5 isolates were DNA sequenced for ITS region. Data analysis showed that there were 2 different isolates (accession number: MN900613 for isolate Bb-Taif1 and MN900614 for isolate Bb-Taif2). The values of LC 50 were 6.041 × 10 4 , 3.199 × 10 5 spores/ml while those of LC 90 were 6.13 × 10 6 and 7.474 × 10 7 spores/ml after 3 days of treatment by Bb-Taif1 and Bb-Taif2, respectively. These values showed that the virulence of Bb-Taif1 isolate was higher in its efficacy than the Bb-Taif2 isolate. Further investigations are needed to evaluate its efficacy on other piercing sucking or chewing insect pests throughout conidia spraying, soil inoculation, and/or root immersion for grapevine plants.

Pathogenicity and Side Effect of Indigenous Beauveria bassiana on Coccinella undecimpunctata and Hippodamia variegata (Coleoptera: Coccinellidae)

“…A concentration of 1 × 10 5 conidia/mL (with 0.02% of Tween 80) was used for all bioassay methods. This concentration was recommended for control of M. rosae [ 24 ] and grapevine aphid [ 25 ]. The control individuals (20 for each replicate) were treated with 1 mL of 0.02% of Tween 80 ( v / v ).…”

New Report of Endophytic Sordariomycetes from the Seagrasses of Cebu, Central Philippines

“…On the other hand, B. bassiana produced iron siderophores and solubilize phosphate, promoting plant growth [39,45]. It is also an active biocontrol agent against agricultural pests [46,47]. It produces bassianolide, beauvericin, and bassiacridin metabolites which are toxic to insects [45].…”

Suitability of five plant species extracts for their compatibility with indigenous Beauveria bassiana against Aphis gossypii Glov. (Hemiptera: Aphididae)

“…A Saudi Arabian indigenous isolate of B. bassiana [Accession numbers: LC338054 for internal transcript spacer (ITS) and LC338058 for cytochrome oxidase I (COI)] was chosen to be used in this study because it had a high virulence against Macrosiphum rosae in laboratory and field experiments (Sayed et al 2019) and Aphis punicae in laboratory experiments (Sayed et al 2020b). The propagation of this isolate was carried out according to Sayed et al (2019) to obtain a sufficient amount for the experiments with a final concentration of 1 × 10 7 spores/ml.…”