Isolation and Differentiation Potential of Human Mesenchymal Stem Cells From Adipose Tissue Harvested by Water Jet-Assisted Liposuction

Meyer, Juliane; Salamon, Achim; Herzmann, Nicole; Adam, Stefanie; Kleine, Hans Dieter; Matthiesen, Inge; Ueberreiter, Klaus; Peters, Kirsten

doi:10.1093/asj/sjv075

Cited by 49 publications

(37 citation statements)

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“…Unlike the immunocompromised nude mice receiving purified ASC cultures,(43) immunocompetent human patients typically receive fat grafts containing a variable, but low (2 to 8%) fraction of ASCs. (44, 45)…”

Section: Discussionmentioning

confidence: 99%

“…Still, various methods of fat harvest and processing may affect adipocyte viability and stem cell fraction. (59–62) While optimization of the ASC-rich stromal vascular fraction of lipoaspirate may favor improved graft retention,(45) it may also increase the risk of exposure of ASCs to occult, residual tumor stroma. (45) All patients were treated at high-volume cancer centers and likely had access to timely cancer treatment and appropriate administration of adjuvant therapy.…”

Section: Discussionmentioning

confidence: 99%

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Cancer Risk after Fat Transfer: A Multicenter Case-Cohort Study

Myckatyn

Wagner

Mehrara

et al. 2017

Plastic &Amp; Reconstructive Surgery

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Background Fat transfer is an increasingly popular method for refining post-mastectomy breast reconstructions. However, concern persists that fat transfer may promote disease recurrence. Adipocytes are derived from adipose-derived stem cells and express adipocytokines that can facilitate active breast cancer cells in laboratory models. We sough to evaluate the association between fat transfer to the reconstructed breast and cancer recurrence in patients diagnosed with local or regional invasive breast cancers. Methods A multi-center, case-cohort study was performed. Eligible patients from four centers (Memorial Sloan Kettering, MD Anderson, Alvin J. Siteman, and the University of Chicago) were identified by each site’s institutional tumor registry or cancer data warehouse. Eligibility criteria were: mastectomy with immediate breast reconstruction between 2006 and 2011, age above 21, female sex, and incident diagnosis of invasive ductal carcinoma, stage I, II or III. Cases consisted of all recurrences during the study period, and controls consisted of a 30% random sample of the study population. Cox proportional hazards regression was used to evaluate for association between fat transfer and time to recurrence in bivariate and multivariate models. Results The time to disease recurrence unadjusted hazard ratio for fat transfer was 0.99 (95% CI: 0.56, 1.7). After adjustment for age, body mass index, stage, HER2/Neu receptor status, and estrogen receptor status, the hazard ratio was was 0.97 (95% CI: 0.54, 1.8). Conclusion In this population of breast cancer patients who had mastectomy with immediate reconstruction, fat transfer was not associated with a higher risk of cancer recurrence.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Cancer Risk after Fat Transfer: A Multicenter Case-Cohort Study

Myckatyn

Wagner

Mehrara

et al. 2017

Plastic &Amp; Reconstructive Surgery

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“…85,86 Preliminary in vitro and rodent studies with water-jet-assisted liposuction suggest that it improves ASC content, results in more viable adipocytes, and increases volume retention of grafts. 87,88 Harvest cannula size has also been investigated to reduce shear stress and improve graft viability. Larger, 5-to 6-mm harvesting cannulas seem to improve adipocyte viability in cell culture and in animal models.…”

Section: Emerging Strategiesmentioning

confidence: 99%

Fat, Stem Cells, and Platelet-Rich Plasma

James

Coleman

Rubin

2016

Clinics in Plastic Surgery

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“…An elegant series of experiments recently demonstrates that lipoaspirates harvested with UAL and SAL share a similar proportion of viable adipose-derived stem cells with equivalent expression of regenerative genes and potential to differentiate into chondrocytes, adipocytes, or osteocytes [11]. Another technology, water jet-assisted liposuction (WAL) uses a dual-aperture cannula that simultaneously emits tumescence solution as a fan-shaped, pulsating jet through an anteriorly located aperture, and suction fluid and aspirated fat from a more posteriorly located side port [35, 46, 59]. Human fat harvested with WAL demonstrated greater viability using both colorimetric and glucose transport test assays, with greater retention by weight, less apoptosis by Annexin V expression, and increased revascularization by CD31 immunostaining when injected as a xenograft into a murine host [59].…”

Section: Discussionmentioning

confidence: 99%

“…Human fat harvested with WAL demonstrated greater viability using both colorimetric and glucose transport test assays, with greater retention by weight, less apoptosis by Annexin V expression, and increased revascularization by CD31 immunostaining when injected as a xenograft into a murine host [59]. Other work has suggested that fat harvested with WAL contains abundant CD34+ cells suggesting a stem cell-rich stromal vascular fraction although a control group was not utilized in this study [35]. Although human data are not available, rats engrafted with human adipose xenografts harvested with TLL demonstrate adipocyte viability, graft weight, and histology comparable to the Coleman technique [9].…”

Section: Discussionmentioning

confidence: 99%

Fat Grafting with Tissue Liquefaction Technology as an Adjunct to Breast Reconstruction

et al. 2016

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BackgroundTissue liquefaction technology (TLT) delivers warmed saline from the liposuction cannula tip at low pressure pulses to disaggregate adipocytes. This technology differs significantly from that used in other liposuction devices including water jet-assisted liposuction. Here we introduce our early experience with this technology in the setting of fat transfer for revision breast reconstruction.MethodsA retrospective chart review of 136 consecutive patients who underwent fat harvest with TLT and subsequent transfer into 237 breast reconstructions was conducted at a single institution. This two-surgeon series examined donor and recipient site complication rates over a median follow-up of 143 days [87–233].ResultsThe overall complication rate was 28.7 %, of which the majority (22.1 %) was fat necrosis at the recipient site as documented by any clinical, imaging, or pathologic evidence. The abdomen served as the donor site for half of the cases. Donor site complications were limited to widespread ecchymosis of the donor site notable in 10.4 % of cases. Twenty-five percent of patients had received postmastectomy radiotherapy prior to fat transfer. Prior to revision with fat transfer, implant-based breast reconstruction was used in 75.5 % of cases, and autologous flaps in the remainder. Fat transfer was combined with other reconstructive procedures 94.1 % of the time.ConclusionsTLT can be used to harvest adipocytes for fat transfer with donor site morbidity and recipient site complications comparable to other modalities. The efficiency and quality of harvested fat makes this technology appealing for wide spread adoption during fat transfer.Level of Evidence IVThis journal requires that the authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.

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Isolation and Differentiation Potential of Human Mesenchymal Stem Cells From Adipose Tissue Harvested by Water Jet-Assisted Liposuction

Abstract: 3 Therapeutic.

Cited by 49 publications

References 49 publications

Cancer Risk after Fat Transfer: A Multicenter Case-Cohort Study

Cancer Risk after Fat Transfer: A Multicenter Case-Cohort Study

Fat, Stem Cells, and Platelet-Rich Plasma

Fat Grafting with Tissue Liquefaction Technology as an Adjunct to Breast Reconstruction

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