Isolation and cultivation of a spirochaete from bovine digital dermatitis

Demirkan, İbrahim; Carter, S. D.; Woodward, Martin J.

doi:10.1136/vr.145.17.497

Cited by 42 publications

(47 citation statements)

References 12 publications

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“…Recently, spirochaetes have been cultured successfully from cases of a related disease of cattle, bovine digital dermatitis, in the USA [11], Germany [12] and the UK [13]. The latter study used immunomagnetic separation to isolate a spirochaete from affected cattle [13]. The present study employed a similar approach in a case of SVOFR and described the morphological, biochemical and genotypic characteristics of the novel treponeme isolated.…”

Section: Introductionmentioning

confidence: 98%

“…The method used was as described previously for the isolation of a spirochaete from a case of bovine digital dermatitis [13]. Brie¯y, immunomagnetic beads (2.8 ìm diameter) covalently coated with polyclonal anti-rabbit IgG were obtained from Dynech (Billingshurst, Sussex).…”

Section: Immunomagnetic Separationmentioning

confidence: 99%

“…Motile spirochaetes were also observed and successfully cultured [9,10]. Recently, spirochaetes have been cultured successfully from cases of a related disease of cattle, bovine digital dermatitis, in the USA [11], Germany [12] and the UK [13]. The latter study used immunomagnetic separation to isolate a spirochaete from affected cattle [13].…”

Section: Introductionmentioning

confidence: 99%

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Isolation and characterisation of a novel spirochaete from severe virulent ovine foot rot

Demirkan¹,

Carter²,

Winstanley³

et al. 2001

Journal of Medical Microbiology

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A novel spirochaete was isolated from a case of severe virulent ovine foot rot (SVOFR) by immunomagnetic separation with beads coated with polyclonal anti-treponemal antisera and prolonged anaerobic broth culture. The as yet unnamed treponeme differs considerably from the only other spirochaete isolated from ovine foot rot as regards morphology, enzymic pro®le and 16S rDNA sequence. On the basis of 16S rDNA, it was most closely related to another unnamed spirochaete isolated from cases of bovine digital dermatitis in the USA, raising the possibility of cross-species transmission. Further information is required to establish this novel ovine spirochaete as the cause of SVOFR.

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Section: Introductionmentioning

confidence: 98%

Section: Immunomagnetic Separationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Isolation and characterisation of a novel spirochaete from severe virulent ovine foot rot

Demirkan¹,

Carter²,

Winstanley³

et al. 2001

Journal of Medical Microbiology

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“…So far, only a limited number of treponemal species from DD lesions have been cultivated in vitro (11,14,19,36,37), and only one, Treponema brennaborense, has been formally classified (31). Consequently, culture-independent phylogenetic methods, such as comparative 16S rRNA gene sequence analysis and fluorescence in situ hybridization (FISH), are valuable tools in determining the diversity, phylogeny, prevalence, and spatial distribution of these unclassified DD spirochetes.…”

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confidence: 99%

Evidence of Multiple Treponema Phylotypes Involved in Bovine Digital Dermatitis as Shown by 16S rRNA Gene Analysis and Fluorescence In Situ Hybridization

et al. 2008

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The etiopathogenesis of the skin disease digital dermatitis (DD), an important cause of lameness in cattle, remains uncertain. Microscopically, the disease appears to be polymicrobial, with spirochetes as the predominant bacteria. The objective of this study was to identify the main part of the bacteria involved in DD lesions of cattle by using culture-independent molecular methods. Ten different phylotypes of Treponema were identified either by 16S rRNA gene sequencing of bacteria from DD lesions or by fluorescence in situ hybridization (FISH) analysis using phylotype-specific 16S rRNA-directed oligonucleotide probes. Two phylotypes, phylotype 1 (PT1) and PT2, were not closely related to any characterized treponemal species. PT7 was 99.3% identical to Treponema denticola, while PT9 resembled T. vincentii by 96%. The remaining phylotypes, PT3, PT4, PT5, PT6, and PT8, and Treponema brennaborense had previously been isolated from DD lesions. Forty DD biopsy specimens were examined for Treponema by FISH. With one exception, all of the biopsy specimens revealed epidermotropic, intermingled infection with three or more different phylotypes (mean, 4.7). The most prevalent species were PT1 (95%), PT6 (93%), and PT3 (85%). While colonization by PT3 was confined to the surface of the epidermis, both PT1 and PT6 invaded deep into the stratum spinosum and were seen in ulcerated dermal papillae. In two cases, all 10 phylotypes were demonstrated. Furthermore, FISH with a Treponema group-specific probe showed that Treponema accounted for more than 90% of the total bacterial population in the biopsy specimens. These data strongly suggest that a group of apparently symbiotic Treponema species are involved as primary bacterial pathogens in DD.

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“…However, despite extensive research, a specific infectious agent has not been identified, and it has been suggested that the disease may be a polymicrobial infection (Döpfer et al, 1997). The range of bacteria cultured from DD lesions includes Campylobacter sputorum (Shibahara et al, 2002), Porphyromonas levii, Fusobacterium necrophorum, Fusobacterium nucleatum, Prevotella oralis, Prevotella denticola, Prevotella bivia, Treponema brennaborense (Schrank et al, 1999) and as-yet unnamed treponemes (Demirkan et al, 1999;Stamm et al, 2002;Walker et al, 1995). By using culture-independent methods, such as immunocytochemistry and 16S rRNA gene sequence analysis, additional organisms were detected in lesions and were related to Bacteroides levii, Borrelia burgdorferi, Mycoplasma hyopharyngis and several Treponema species (Collighan & Woodward, 1997; Demirkan et al, 1998; Moter et al, 1998).…”

Section: Tierarztpraxis Ch-3186 Dü Dingen Switzerlandmentioning

confidence: 99%

Guggenheimella bovis gen. nov., sp. nov., isolated from lesions of bovine dermatitis digitalis

Wyss

Dewhirst²,

Paster³

et al. 2005

International Journal of Systematic and Evolutionary Microbiology

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Tierarztpraxis, CH-3186 Dü dingen, SwitzerlandDermatitis digitalis is an economically important ulcerative disease of undetermined aetiology affecting the hooves of cattle. Material was examined from two independent cases of this disease in Switzerland. Cultures from the advancing front of both lesions yielded large numbers of closely related short, mesophilic, non-motile, non-spore-forming, anaerobic, proteolytic, Gram-positive rods. The 16S rRNA gene sequences of strains OMZ 913 T and OMZ 915 were identical and indicate Tindallia magadiensis and Eubacterium saphenum as their closest relatives. Phenotypically, the novel isolates are clearly distinguished from related bacteria by protein and antigen patterns, by cellular fatty acids and by API ZYM activities. The diamino acid of the Gram-positive cell wall is ornithine and the G+C content of OMZ 913 T DNA is 44?4 mol%. The phylogenetic distance from recognized taxa in the phylum Firmicutes is sufficient to place these bovine isolates into a novel genus and species, for which the name Guggenheimella bovis gen. nov., sp. nov. is proposed, with OMZ 913 T (=CIP 108087 T =DSM 15657 T ) as the type strain.Dermatitis digitalis (DD), also known as Mortellaro disease or strawberry foot, is an economically important ulcerative disease affecting the bovine foot in an increasing number of countries including Germany, Italy, Japan, the Netherlands, Switzerland, the UK and the USA and may be the dominant cause of lameness in dairy cows (Blowey & Sharp, 1988;Choi et al., 1997;Collighan & Woodward, 1997; Collighan et al., 2000; Demirkan et al., 1998; Luginbühl & Kollbrunner, 2000; Moter et al., 1998;Schrank et al., 1999;Shibahara et al., 2002;Walker et al., 1995). The reported spread between herds by veterinarians, foot trimmers and purchased animals as well as curing of the condition by antibiotic therapy are characteristics of an infectious disease (Laven, 2001). However, despite extensive research, a specific infectious agent has not been identified, and it has been suggested that the disease may be a polymicrobial infection (Döpfer et al., 1997). The range of bacteria cultured from DD lesions includes Campylobacter sputorum (Shibahara et al., 2002), Porphyromonas levii, Fusobacterium necrophorum, Fusobacterium nucleatum, Prevotella oralis, Prevotella denticola, Prevotella bivia, Treponema brennaborense (Schrank et al., 1999) and as-yet unnamed treponemes (Demirkan et al., 1999;Stamm et al., 2002;Walker et al., 1995). By using culture-independent methods, such as immunocytochemistry and 16S rRNA gene sequence analysis, additional organisms were detected in lesions and were related to Bacteroides levii, Borrelia burgdorferi, Mycoplasma hyopharyngis and several Treponema species (Collighan & Woodward, 1997; Demirkan et al., 1998; Moter et al., 1998). Treponemes have been detected deep in the affected tissue by in situ hybridization using fluorescent 16S rRNAtargeted oligonucleotide probes; however, the eubacterial organisms seen at the front of the lesion appeared not to b...

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Isolation and cultivation of a spirochaete from bovine digital dermatitis

Cited by 42 publications

References 12 publications

Isolation and characterisation of a novel spirochaete from severe virulent ovine foot rot

Isolation and characterisation of a novel spirochaete from severe virulent ovine foot rot

Evidence of Multiple Treponema Phylotypes Involved in Bovine Digital Dermatitis as Shown by 16S rRNA Gene Analysis and Fluorescence In Situ Hybridization

Guggenheimella bovis gen. nov., sp. nov., isolated from lesions of bovine dermatitis digitalis

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