Isolation and Characterization of Two New Metabolites from the Sponge-Derived Fungus Aspergillus sp. LS34 by OSMAC Approach

Li, Wei; Ding, Lijian; Wang, Ning; Xu, Jianzhou; Zhang, Weiyan; Zhang, Bin; He, Shan; Wu, Bin; Jin, Haixiao

doi:10.3390/md17050283

Cited by 22 publications

(17 citation statements)

References 27 publications

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“…Natural product Altered conditions [17] Additives such as solvents, heavy metals, tensides, precursors, and other small molecule elicitors are also useful for the elicitation of secondary metabolite production in microorganisms. Chen reports that organic solvents can either increase (DMSO, EtOH) or decrease (THF, DMF) the production of antibiotics such as tetracenomycin and thiostrepton in Streptomyces strains [18].…”

Section: Strainmentioning

confidence: 99%

Triaging of Culture Conditions for Enhanced Secondary Metabolite Diversity from Different Bacteria

Schwarz¹,

Lütz²

2020

Preprint

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Over the past decade, the One Strain Many Compounds (OSMAC) approach has been established for silent gene cluster activation and elicitation of secondary metabolite production, but so far the full secondary metabolome of a biosynthetically promising bacterium has not been elucidated yet. Here, we investigate the ability of seven categories of OSMAC conditions to enhance the diversity of new mass features from bacterial strains with little literature coverage but high biosynthetic potential. The strains Bacillus. amyloliquefaciens DSM7, Corallococcus. coralloides DSM2259, Pyxidicoccus. fallax HKI727, Rhodococcus. jostii DSM44719, and Streptomyces. griseochromogenes DSM40499 were selected after genome mining with antiSMASH. After cultivation under OSMAC conditions, the generated extracts were subjected to LC-MS and MZmine analysis to determine new mass features and evaluate the tested culture conditions. 4 predicted compounds, bacillibactin, desferrioxamine B, myxochelin A, and surfactin, were identified and up to 147 new mass features were detected in the generated extracts, which greatly surpasses the number of predicted gene clusters. Among the new mass features are bioactive compounds that were so far unreported for the strains such as cyclo-(Tyr-Pro) from DSM7 and nocardamin from DSM2259. Furthermore, the tested culture conditions were evaluated regarding their suitability for the generation of new mass features from the selected strains and promising new starting points for further screenings are postulated. Especially culture conditions with little prior literature coverage are responsible for the activation of predicted gene clusters

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Section: Strainmentioning

confidence: 99%

Triaging of Culture Conditions for Enhanced Secondary Metabolite Diversity from Different Bacteria

Schwarz¹,

Lütz²

2020

Preprint

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“…The in vitro cytotoxic activities of all isolated compounds against four human cancer cell lines (CCRF-CEM, MDA-MB-231, HCT-116, and AGS) were evaluated by the MTT method as previously described (Huang et al, 2019;Li W. et al, 2019). 7-Ethyl-10hydroxycamptothecin (1.3, 10.8, 9.9, and 4.2 nM, respectively) was used as the positive control against four above-mentioned human cancer cell lines.…”

Section: In Vitro Cancer Cell Cytotoxicity Assaysmentioning

confidence: 99%

“…As part of a continuing research program investigating the biologically active secondary metabolites from sponge-derived fungi (Ding et al, 2018;Huang et al, 2019;Li W. et al, 2019), a detailed chemical investigation was initiated on the culture of fungus Aspergillus sp. NBUF87.…”

Section: Introductionmentioning

confidence: 99%

New Dihydroisocoumarin Root Growth Inhibitors From the Sponge-Derived Fungus Aspergillus sp. NBUF87

Huang

Ding

et al. 2019

Front. Microbiol.

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Six new dihydroisocoumarins, aspergimarins A−F (1−6), were discovered together with five known analogs (7−11) from a monoculture of the sponge-derived fungus Aspergillus sp. NBUF87. The structures of these compounds were elucidated through comprehensive spectroscopic methods, and absolute configurations were assigned after X-ray crystallography, use of the modified Mosher's method, and comparison of electronic circular dichroism (ECD) data with literature values for previously reported analogs. Compounds 1−11 were evaluated in a variety of bioassays, and at 100 µM, both 1 and 5 showed significant inhibitory effects on the lateral root growth of Arabidopsis thaliana Columbia-0 (Col-0). Moreover, at 100 µM, 5 also possessed notable inhibition against the primary root growth of Col-0. Meanwhile, 1−11 were all found to be inactive in vitro against acetylcholinesterase (AChE) (IC 50 > 100 µM), four different types of human-derived cancer cell lines (IC 50 > 50 µM), as well as methicillin-resistant Staphylococcus aureus and Escherichia coli (MIC > 50 µg/mL), and Plasmodium falciparum W2 (EC 50 > 100 µg/mL), in phenotypic tests.

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“…Among these, algae-derived fungi have been reported to be the largest source of secondary metabolites with diversified bioactivities [1,2], and that can be exploited potentially as lead compounds for new drug development. It was reported that by employing one of the post-genomic strategies, one strain many compounds (OSMAC), on the cultivation of the fungal strains could enhance the quantity and diversity of fungal secondary metabolites [3,4]. The OSMAC approach usually involved the manipulation of culturing parameters, such as media formulation, temperature, agitation, luminosity, aeration, etc.…”

Section: Introductionmentioning

confidence: 99%

“…enhance the quantity and diversity of fungal secondary metabolites [3,4]. The OSMAC approach usually involved the manipulation of culturing parameters, such as media formulation, temperature, agitation, luminosity, aeration, etc.…”

Section: Introductionmentioning

confidence: 99%

Highly Oxygenated Constituents from a Marine Alga-Derived Fungus Aspergillus giganteus NTU967

et al. 2020

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Agar-based disc diffusion antimicrobial assay has shown that the ethyl acetate extract of the fermented broth of Aspergillus giganteus NTU967 isolated from Ulva lactuca exhibited significant antimicrobial activity in our preliminary screening of bioactive fungal strains. Therefore, column chromatography of the active principles from liquid- and solid–state fermented products of the fungal strain was carried out, and which had led to isolation of eleven compounds. Their structures were determined by spectral analysis to be seven new highly oxygenated polyketides, namely aspergilsmins A–G (1–7), along with previously reported patulin, deoxytryptoquivaline, tryptoquivaline and quinadoline B. Among these, aspergilsmin C (3) and patulin displayed promising anticancer activities against human hepatocellular carcinoma SK-Hep-1 cells and prostate cancer PC-3 cells with IC50 values between 2.7–7.3 μM. Furthermore, aspergilsmin C (3) and patulin exhibited significant anti-angiogenic functions by impeding cell growth and tube formation of human endothelial progenitor cells without any cytotoxicity.

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Isolation and Characterization of Two New Metabolites from the Sponge-Derived Fungus Aspergillus sp. LS34 by OSMAC Approach

Cited by 22 publications

References 27 publications

Triaging of Culture Conditions for Enhanced Secondary Metabolite Diversity from Different Bacteria

Triaging of Culture Conditions for Enhanced Secondary Metabolite Diversity from Different Bacteria

New Dihydroisocoumarin Root Growth Inhibitors From the Sponge-Derived Fungus Aspergillus sp. NBUF87

Highly Oxygenated Constituents from a Marine Alga-Derived Fungus Aspergillus giganteus NTU967

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