Isolation and characterization of two YUCCA flavin monooxygenase genes from cultivated strawberry (Fragaria × ananassa Duch.)

Liu, Hong; Ying, Yu; Zhang, Ling; Gao, Qing‐Hua; Li, Jing; Zhang, Zhen; Fang, Jinggui; Ke, Danxia

doi:10.1007/s00299-012-1258-4

Cited by 35 publications

(21 citation statements)

References 41 publications

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“…In this work, IiYUC6 presented the highest expression level in fruit compared with other tissues. Similar results were observed from strawberry in which FaYUC1-2 might be involved in flower and fruit development processes (Liu et al, 2012). Therefore, our results suggested that IiYUC6 could play an important role in fruit development processes in I. indigotica .…”

Section: Discussionsupporting

confidence: 82%

“…YUCCA ( YUC ) encodes FMOs that could convert indole-3-pyruvate (IPA) to indole-3-acetic acid (IAA) and was identified in Arabidopsis (Kim et al, 2007), Zea mays (Li et al, 2015), Cucumis sativus (Yan et al, 2016), Glycine max (Costa et al, 2016), Triticum aestivum (Li N. et al, 2014), Fragaria × ananassa Duch (Liu et al, 2012), and Oryza sativa (Abu-Zaitoon, 2014). Based on YUCCA protein, a similar sequence from the transcriptome data of I. indigotia was found in the Full-Length CDS DataBase, and the expression patterns of IiYUC6 were discussed.…”

Section: Discussionmentioning

confidence: 99%

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Selection and Validation of Appropriate Reference Genes for qRT-PCR Analysis in Isatis indigotica Fort.

Li¹,

Jing²,

Lu³

et al. 2017

Front. Plant Sci.

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Due to its sensitivity and specificity, real-time quantitative PCR (qRT-PCR) is a popular technique for investigating gene expression levels in plants. Based on the Minimum Information for Publication of Real-Time Quantitative PCR Experiments (MIQE) guidelines, it is necessary to select and validate putative appropriate reference genes for qRT-PCR normalization. In the current study, three algorithms, geNorm, NormFinder, and BestKeeper, were applied to assess the expression stability of 10 candidate reference genes across five different tissues and three different abiotic stresses in Isatis indigotica Fort. Additionally, the IiYUC6 gene associated with IAA biosynthesis was applied to validate the candidate reference genes. The analysis results of the geNorm, NormFinder, and BestKeeper algorithms indicated certain differences for the different sample sets and different experiment conditions. Considering all of the algorithms, PP2A-4 and TUB4 were recommended as the most stable reference genes for total and different tissue samples, respectively. Moreover, RPL15 and PP2A-4 were considered to be the most suitable reference genes for abiotic stress treatments. The obtained experimental results might contribute to improved accuracy and credibility for the expression levels of target genes by qRT-PCR normalization in I. indigotica.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Selection and Validation of Appropriate Reference Genes for qRT-PCR Analysis in Isatis indigotica Fort.

Li¹,

Jing²,

Lu³

et al. 2017

Front. Plant Sci.

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“…A comparable peak in expression was also observed for YUC1 (8–10 wpf) (Figure 7D), whereas YUC3 was found to be expressed at very low levels in young berries (one and two wpf), but YUC3 transcripts could not be reliably detected in berries 3–16 wpf (data not shown). An increase in the expression of YUC genes, similar to YUC1 , has recently been reported in strawberries, where the transcript accumulation of Fa YUC1 and Fa YUC2 in achenes peaked at the large green fruit stage [73]. In grape berries, the increase in expression of the putative auxin-biosynthesis genes was preceded by high expression levels of the ethylene biosynthesis genes ACS1 (3–7 wpf) and ACO1 (peaking at 7 wpf) (Figure 7D) which confirmed previous results for the expression of ACS/ACO in developing grape berries [12,14,15].…”

Section: Resultsmentioning

confidence: 90%

Interactions between ethylene and auxin are crucial to the control of grape (Vitis vinifera L.) berry ripening

et al. 2013

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BackgroundFruit development is controlled by plant hormones, but the role of hormone interactions during fruit ripening is poorly understood. Interactions between ethylene and the auxin indole-3-acetic acid (IAA) are likely to be crucial during the ripening process, since both hormones have been shown to be implicated in the control of ripening in a range of different fruit species.ResultsGrapevine (Vitis vinifera L.) homologues of the TRYPTOPHAN AMINOTRANSFERASE RELATED (TAR) and YUCCA families, functioning in the only characterized pathway of auxin biosynthesis, were identified and the expression of several TAR genes was shown to be induced by the pre-ripening application of the ethylene-releasing compound Ethrel. The induction of TAR expression was accompanied by increased IAA and IAA-Asp concentrations, indicative of an upregulation of auxin biosynthesis and conjugation. Exposure of ex planta, pre-ripening berries to the ethylene biosynthesis inhibitor aminoethoxyvinylglycine resulted in decreased IAA and IAA-Asp concentrations. The delayed initiation of ripening observed in Ethrel-treated berries might therefore represent an indirect ethylene effect mediated by increased auxin concentrations. During berry development, the expression of three TAR genes and one YUCCA gene was upregulated at the time of ripening initiation and/or during ripening. This increase in auxin biosynthesis gene expression was preceded by high expression levels of the ethylene biosynthesis genes 1-aminocyclopropane-1-carboxylate synthase and 1-aminocyclopropane-1-carboxylate oxidase.ConclusionsIn grape berries, members of both gene families involved in the two-step pathway of auxin biosynthesis are expressed, suggesting that IAA is produced through the combined action of TAR and YUCCA proteins in developing berries. The induction of TAR expression by Ethrel applications and the developmental expression patterns of auxin and ethylene biosynthesis genes indicate that elevated concentrations of ethylene prior to the initiation of ripening might lead to an increased production of IAA, suggesting a complex involvement of this auxin and its conjugates in grape berry ripening.

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“…1C), which would result in a 142G . D amino acid substitution within the ATG-containing motif 1 conserved between YUCCA proteins (Liu et al, 2012). A single 628G .…”

Section: Resultsmentioning

confidence: 99%

Linking Auxin with Photosynthetic Rate via Leaf Venation

McAdam

Eléouët

Best³

et al. 2017

Plant Physiol.

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Land plants lose vast quantities of water to the atmosphere during photosynthetic gas exchange. In angiosperms, a complex network of veins irrigates the leaf, and it is widely held that the density and placement of these veins determines maximum leaf hydraulic capacity and thus maximum photosynthetic rate. This theory is largely based on interspecific comparisons and has never been tested using vein mutants to examine the specific impact of leaf vein morphology on plant water relations. Here we characterize mutants at the Crispoid (Crd) locus in pea (Pisum sativum), which have altered auxin homeostasis and activity in developing leaves, as well as reduced leaf vein density and aberrant placement of free-ending veinlets. This altered vein phenotype in crd mutant plants results in a significant reduction in leaf hydraulic conductance and leaf gas exchange. We find Crispoid to be a member of the YUCCA family of auxin biosynthetic genes. Our results link auxin biosynthesis with maximum photosynthetic rate through leaf venation and substantiate the theory that an increase in the density of leaf veins coupled with their efficient placement can drive increases in leaf photosynthetic capacity.

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Isolation and characterization of two YUCCA flavin monooxygenase genes from cultivated strawberry (Fragaria × ananassa Duch.)

Cited by 35 publications

References 41 publications

Selection and Validation of Appropriate Reference Genes for qRT-PCR Analysis in Isatis indigotica Fort.

Selection and Validation of Appropriate Reference Genes for qRT-PCR Analysis in Isatis indigotica Fort.

Interactions between ethylene and auxin are crucial to the control of grape (Vitis vinifera L.) berry ripening

Linking Auxin with Photosynthetic Rate via Leaf Venation

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