Isolation and characterization of twenty microsatellite loci for the ballan wrasse, Labrus bergylta

Abstract

We developed 20 nuclear microsatellite DNA markers from tri-and tetra-nucleotide enriched libraries in the ballan wrasse. In our dataset (N = 241), the detected number of alleles per locus ranged from 2 to 12, and the observed and expected heterozygosity varied from 0.251 to 0.778 and from 0.286 to 0.804, respectively. Cross amplification with the goldsinny wrasse resulted in two usable loci whereas the corkwing wrasse amplified in one locus. The ballan wrasse is an important resource for aquaculture as it delouses farmed salmon efficiently and removes organic matter from farming facilities. In order to obtain individuals for this industry, the wrasse are translocated along the Norwegian coastline, in spite of no knowledge of the population structure. This paper enables such studies, which might be important for the long term management of the species.

Is the ballan wrasse (Labrus bergylta) two species? Genetic analysis reveals within-species divergence associated with plain and spotted morphotype frequencies

“…Thus, while 2 of the samples failed to amplify for COI, they provided microsatellite profiles consistent with the species (Quintela et al 2014). A total of 150 microsatellite alleles across the 20 markers were observed in the 89 samples.…”

“…Finally, a remarkable feature is that Galician ballan wrasse analyzed here showed a substantially high- 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 Genetic analysis of ballan wrasse morphotypes 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 er number of alleles than an almost 3-fold larger set of individuals sampled in the Norwegian coast (150 vs 115 alleles, respectively) (Quintela et al 2014). Although this number of populations study does not allow any further assumptions to be made, we would like to point out the fact that also in the control region of mtD-NA, a lower genetic diversity has been formerly reported for this species in the northernmost areas when comparing southern Norway and the British Isles, which was interpreted as the result of historical demographic events (population bottleneck followed by expansion) (D'Arcy et al 2013).…”

“…Twenty microsatellite loci (WrA103, WrA107, WrA111, WrA112, WrA113, WrA203, WrA223, WrA224, WrA228, WrA236, WrA237, WrA254, WrA255, WrA256, WrA259, WrA261, WrB102, WrB212, WrB213, WrB215) were used as described in Quintela et al (2014). Six multiplexed PCR reactions were performed in final volume of 10 μL containing 50 ng DNA template, 1× buffer, 2 mM MgCl 2 , 1.25 mM dNTPs, 0.06-0.12 μM of each primer and 1U Go-Taq polymerase.…”

“…Recently, we developed a set of 20 microsatellite markers that enables genetic studies of this species (Quintela et al 2014). Here, the main aim was to evaluate whether there were any genetic differences between plain and spotted morphotypes of ballan wrasse in order to investigate the existence and, eventually, the degree of disruptive selection between color patterns as well as to measure the gene flow between them.…”

On the absence of genetic differentiation between morphotypes of the ballan wrasse Labrus bergylta (Labridae)

“…Further studies are needed to address the life history differences between plain and spotted morphotypes. A set of 20 microsatellite loci was recently described for this species by Quintela et al ( 2014 ) which will also allow for a detailed study of gene flow and recent population differentiation.…”