Isolation and characterization of thirteen novel dinucleotide microsatellite loci from Tetraena mongolica Maxim

Zhi, Yingbiao; Ding, Ling; Wang, Yupeng; Hu, Longfei; Hua, Yupeng; Zhao, Kai; He, Jinliang; Yang, Chao; Zhang, Baowei

doi:10.1007/s12686-013-0135-y

Cited by 3 publications

(2 citation statements)

References 2 publications

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“…Total genomic DNA was extracted from the powdered tissue following a modified CTAB procedure ( Doyle & Doyle, 1987 ), and purified via an EasyPure PCR Purification Kit (TransGene). In the present study, we used twelve high polymorphic loci for T. mongolica ( Zhi et al, 2014 ) as genetic markers. PCR reaction mixtures (25 µL) consisted of 1 µL genomic DNA (concentration 10–50 ng/µL), 2 µL 10 × buffer, 1 µL of 2.5 mM MgSO 4 , 2 µL of 2 mM dNTPs, 1 U Taq polymerase, 0.3 mM of each primer (forward primer fluorescently labeled with FAM, HEX or TAMRA) and sufficient water.…”

Section: Methodsmentioning

confidence: 99%

How much genetic variation is stored in the endangered and fragmented shrubTetraena mongolicaMaxim?

Zhi

Sun

et al. 2018

PeerJ

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Tetraena mongolica Maxim (Zygophyllaceae) is an endangered species endemic to western Inner Mongolia and China, and is currently threatened by habitat loss and human over-exploitation. We explored the genetic background, its genetic diversity, population structure, and demographic history, based on 12 polymorphic nuclear microsatellite loci. Our results indicated high genetic diversity in extant populations, but no distinguishable gene cluster corresponding with a specific biogeography. Population demography analysis using a MSVAR indicated a strong, recent population decline approximately 5,455 years ago. These results suggest that the Yellow River and Zhuozi Mountain range may not prevent pollination between populations. Finally, we surmised that the population demography of T. mongolica was likely to have been affected by early mankind activities.

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Section: Methodsmentioning

confidence: 99%

How much genetic variation is stored in the endangered and fragmented shrubTetraena mongolicaMaxim?

Zhi

Sun

et al. 2018

PeerJ

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“…In the present study, 63 positive clones from 432 plasmids were sequenced. Although we obtained many sequences with repeats, a proportion of them could not be used to design primers for several reasons, such as annealing temperatures that were too high or low (Zhi et al, 2014), or repeats that were too short. In addition, some sequences could not be amplified with the primers.…”

Section: Resultsmentioning

confidence: 99%

Development of microsatellite markers for the Siberian weasel <i>Mustela sibirica</i>

et al. 2018

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The Siberian weasel (Mustela sibirica) is widely distributed in mainland Asia, but its introduction into Japan and subsequent expansion have affected the Japanese weasel (M. itatsi). To provide a useful tool for population genetic studies and control of M. sibirica, we developed 10 polymorphic microsatellite markers. Among 40 individuals of M. sibirica collected in Hubei Province, China, the number of alleles per locus varied from 2 to 19, with the observed heterozygosity ranging from 0.050 to 1.000 and the expected heterozygosity ranging from 0.049 to 0.920. None of the loci deviated from Hardy-Weinberg equilibrium. These markers will be useful in further studies investigating the population structure and natural history of M. sibirica, and may thus provide new insights for the efficient management of this species.

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Isolation and characterisation of 16 novel polymorphic microsatellite loci for the Eurasian coot (Fulica atra)

Zhu

Xue

et al. 2016

Aust. J. Zool.

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To study the genetic diversity of the Eurasian coot (Fulica atra), 16 novel microsatellites were isolated and characterised using a (AC)12-enriched genomic library. Polymorphism of each locus was detected in 19 individuals from Weishan Lake and Zhaoyang Lake, both located in Shandong Province, China. These microsatellite loci revealed 3–12 alleles in two populations. One locus (Fa6) showed significant deviations from Hardy–Weinberg equilibrium in the Zhaoyang Lake population. By means of Principal Coordinates Analysis, 19 individuals were divided into two groups, which verified they were from two populations. These novel microsatellites will be useful for further studies and genetic analyses of the Eurasian coot.

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Isolation and characterization of thirteen novel dinucleotide microsatellite loci from Tetraena mongolica Maxim

Cited by 3 publications

References 2 publications

How much genetic variation is stored in the endangered and fragmented shrubTetraena mongolicaMaxim?

How much genetic variation is stored in the endangered and fragmented shrubTetraena mongolicaMaxim?

Development of microsatellite markers for the Siberian weasel <i>Mustela sibirica</i>

Isolation and characterisation of 16 novel polymorphic microsatellite loci for the Eurasian coot (Fulica atra)

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