Development of microsatellite markers for the Siberian weasel &lt;i&gt;Mustela sibirica&lt;/i&gt;

Zhao, Baojun; Xue, Song; Zhu, Wei; Lv, Tianshu; Wei, Qinguo; Shang, Shuai; Zhang, Honghai

doi:10.1266/ggs.18-00018

Cited by 2 publications

(2 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The species is listed as Least Concern on the IUCN Red List (Abramov et al 2016). The previous studies on M. sibirica mainly focused on its biological characteristics, ecology, and genetic diversity (Suzuki et al 2013;Son et al 2017;Zhao et al 2019). However, not many studies have examined the complete mitogenome of M. sibirica in Northern China.…”

Section: Mustela Sibirica; Mustelidae; Mitochondrial Genome; Phylogenymentioning

confidence: 99%

Complete mitochondrial genome ofMustela sibirica(Carnivora: Mustelidae), a protected and endangered species in China

Gao

Lü

Liang

et al. 2020

Mitochondrial DNA Part B

View full text Add to dashboard Cite

We sequenced the complete mitochondrial genome (mitogenome) of Mustela sibirica in China by the shotgun genome skimming methods. The mitogenome of M. sibirica is 16,558bp in length with the base composition of 32.9% A, 27.3% T, 26.0% C, and 13.9% G, and consists of 13 protein-coding genes (PCGs), 22 tRNAs, two rRNAs, and one non-coding control region. The 13 PCGs use ATG as initiation codons except ND3 , ND5 and ND2 which initiate with codons ATA and ATT, respectively. Four ( COX3 , ND1 , ND2 and ND4 ) of the 13 PCGs terminate with a single T– –, and the remainder with a TAA termination codon except ND3 and CYT B using TA– and AGA as termination codon. The phylogenetic tree based on 13 protein-coding genes indicated that M. sibirica is sister to the clade grouped with three species M. nigripes , M. eversmannii , and M. putorius , and Mustelinae species formed a monophyletic group, which is close to the Lutrinae clade within Mustelidae.

show abstract

Section: Mustela Sibirica; Mustelidae; Mitochondrial Genome; Phylogenymentioning

confidence: 99%

Complete mitochondrial genome ofMustela sibirica(Carnivora: Mustelidae), a protected and endangered species in China

Gao

Lü

Liang

et al. 2020

Mitochondrial DNA Part B

View full text Add to dashboard Cite

show abstract

“…Beugin et al, 2017; McFarlane et al, 2020). There are also several studies with microsatellites to distinguish between Mustela species (Cabria et al, 2007; Fleming et al, 1999; Zhao et al, 2018), but there exists only one single study aimed at exploring the dynamics of hybridization (Cabria et al, 2011). The only published hybridization study in mustelids using a genomic approach examined admixture between divergent lineages of stoat ( M. erminea ) in a phylogeographic study (Colella et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

A comparison of microsatellites and genome‐wide SNPs for the detection of admixture brings the first molecular evidence for hybridization between Mustela eversmanii and M. putorius (Mustelidae, Carnivora)

Szatmári

Cserkész

Laczkó

et al. 2021

Evolutionary Applications

View full text Add to dashboard Cite

Introgressive hybridization can pose a serious threat to endangered species which have an overlapping distribution such as in the case of two polecat species, Mustela eversmanii and M. putorius, in Europe. The population size of steppe polecat is known to continuously shrink, whereas its sister species, the European polecat, is still somehow widespread. In this study, we perform an analysis using microsatellite (SSR) and genomic (SNP) data sets to identify natural hybrids between polecats. Four populations were genotyped for eight polymorphic SSR loci, and thousands of unlinked SNPs were generated using a reduced‐representation sequencing approach, RADseq, to characterize the genetic make‐up of allopatric populations and to identify hybrids in the sympatric area. We applied standard population genetic analyses to characterize the populations based on their SSR allelic frequency. Only a single sample out of 48 sympatric samples showed exact intermediacy that we identified as an F1 hybrid. Additionally, one specimen was indicated in the genomic data sets as backcrossed. Other backcrosses, indicated by SSRs, were not validated by SNPs, which highlights the higher efficacy of the genomic method to identify backcrossed individuals. The low frequency of hybridization suggests that the difference in habitat preference of the two species may act as a barrier to admixture. Therefore, it is apparently unlikely that polecat populations are threatened by significant introgression. The two species showed a clear genetic differentiation using both techniques. We found higher genetic diversity values in the sympatric steppe polecat population than in the other studies on polecat populations. Although M. putorius is a hunted species in most countries, genetic diversity values indicate worse conditions in Europe than in the protected sibling species M. eversmanii. Suspending hunting and providing protected status of the former seems to be reasonable and timely.

show abstract

Development of microsatellite markers for the Siberian weasel <i>Mustela sibirica</i>

Cited by 2 publications

References 18 publications

Complete mitochondrial genome ofMustela sibirica(Carnivora: Mustelidae), a protected and endangered species in China

Complete mitochondrial genome ofMustela sibirica(Carnivora: Mustelidae), a protected and endangered species in China

A comparison of microsatellites and genome‐wide SNPs for the detection of admixture brings the first molecular evidence for hybridization between Mustela eversmanii and M. putorius (Mustelidae, Carnivora)

Contact Info

Product

Resources

About