Isolation and Characterization of Thermo-tolerant Phosphate-Solubilizing Bacteria From a Phosphate Mine and Their Rock Phosphate Solubilizing Abilities

Yadav, H.; Gothwal, Raj Kumar; Solanki, Pooran Singh; Nehra, Sampat; Sinharoy, Senjuti; Ghosh, Purnendu

doi:10.1080/01490451.2014.943856

Cited by 19 publications

(5 citation statements)

References 28 publications

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“…The observed mechanism of Mn solubilization is mostly due to the fungal production of bio generated organic acids such as oxalic acid, citric acid, maleic acid and gluconic acid as revealed by ion chromatography analysis ( Figure 5) (Metrohm 883 IC Plus). Similar reports on acid generated leaching of metal has been previously reported by (Yadava et al 2014;Das et al 2012). …”

Section: Screening For Manganese Solubilizing Abilitysupporting

confidence: 85%

Isolation, Identification and Screening of Manganese Solubilizing Fungi From Low-Grade Manganese Ore Deposits

Mohanty

Ghosh

Nayak

et al. 2016

Geomicrobiology Journal

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The present investigation reports the isolation, molecular identification and screening of Mn solubilizing fungal strains from low grade Mn mine tailings. Six morphologically distinct Mn solubilizing fungal strains were isolated on MnO 2 supplemented agar plates with Mn concentration of 0.1% (w/v).The biochemical characterization of the isolated fungal strains was carried out. The molecular identification by Internal Transcribed Spacer (ITS) sequencing identified the strains as Aspergillus terreus, Aspergillus oryzae, Penicillium species, Penicillium species, Penicillium dalea, and Penicillium species with GenBank accession numbers KP309809, KP309810, KP309811, KP309812, KP309813 and KP309814 respectively. The ability of the isolated fungal strains to tolerate and solubilize Mn was investigated by sub-culturing them on Mn supplemented plates with concentration ranging from 0.1-0.5% (w/v). Mn solubilizing ability of the fungal isolates is possibly due to the mycelia production of bio generated organic acids such as oxalic acid, citric acid, maleic acid and gluconic acid as revealed by ion chromatography. Our investigation signifies the role of fungi in biotransformation of insoluble Mn oxide.

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Section: Screening For Manganese Solubilizing Abilitysupporting

confidence: 85%

Isolation, Identification and Screening of Manganese Solubilizing Fungi From Low-Grade Manganese Ore Deposits

Mohanty

Ghosh

Nayak

et al. 2016

Geomicrobiology Journal

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“…Some reports indicate that the type and amount of organic acids produced can be influenced by insoluble phosphate forms used in the cultures 48 . Yadav et al 49 . and Chawngthu et al 50 .…”

Section: Discussionmentioning

confidence: 99%

“…Some reports indicate that the type and amount of organic acids produced can be influenced by insoluble phosphate forms used in the cultures. 48 Yadav et al 49 and Chawngthu et al 50 reported the production of several known organic acids (succinic, oxalic, malic, acetic, tartaric, gluconic, formic and citric acid) by PSBs in the culture supernatant, along with some unknown acids. Sun et al 51 suggested that phosphate solubilization by PSBs was likely due to the secretion of gluconic acid and oxalic acid, which led to a pH decrease to approximately 4.0.…”

Section: Discussionmentioning

confidence: 99%

Growth promotion ability of phosphate‐solubilizing bacteria from the soybean rhizosphere under maize–soybean intercropping systems

et al. 2021

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BACKGROUND Optimum cultivation and management measures are needed to increase the phosphorus (P) absorption efficiency of crops for sustainable agricultural production. Previous studies indicated that leguminous crops can promote P absorption by neighboring gramineous crops. In this study, we isolated and screened the phosphate‐solubilizing bacteria (PSB) from soybean rhizosphere under a maize–soybean intercropping system in Southwest China, and nine PSBs with high P‐solubilizing ability were identified. RESULTS 16S rDNA sequencing and phylogenetic analysis showed that these PSBs belong mainly to Bacillus and Pseudomonas. The phosphate solubility of Bacillus aryabhattai B8W22 reached 388.62 μg mL−1. High‐performance liquid chromatographic analysis showed that each strain could secrete a large quantity of organic acids, including oxalic acid, malonic acid, citric acid and succinic acid. In addition, all strains produced indole acetic acid (IAA) and siderophores that could promote plant growth. Seed germination experiments testified that PSBs isolated in this study have an innate ability to promote plant growth. The plant culture pot experiment further illustrated that soil acid phosphatase (ACP) activity and available P content, as well as plant P uptake, increased significantly with PSBs inoculation. CONCLUSION PSBs from the rhizosphere soil of intercropped soybean could secrete organic acids that increase the solubilization of unavailable P, improve soil ACP activity and P availability, and produce IAA and siderophores that promote maize seed germination and seedling growth. Our findings indicate the PSBs from soybean rhizosphere have significant potential to reduce the application of chemical phosphate fertilizers and to promote sustainable agricultural development. © 2021 Society of Chemical Industry.

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“…PSMs fractionate insoluble P forms into soluble forms through various biological mechanisms, including the production of organic acids and extracellular enzymes, which convert insoluble forms of P into forms available for plant absorption (Hanif et al, 2015;Li et al, 2015;Baliah et al, 2016;Gurikar et al, 2016;Doilom et al, 2020;Sarr et al, 2020;Zúñiga-Silgado et al, 2020). The organic acids produced include glycolic, 2-ketogluconic, acetic, citric, propionic, succinic, tartaric, formic, fumaric, lactic, malic, butyric, gluconic, valeric, oxalic, and citric acids (Hwangbo et al, 2003;Chen et al, 2006;Patel et al, 2008;Scervino et al, 2010;Zhu et al, 2012;Jog et al, 2014;Mehta et al, 2015;Yadav et al, 2015). However, despite their importance, few PSM strains are currently available in the global market for inoculants.…”

Section: Introductionmentioning

confidence: 99%

Efficiency of the Hydroponic System as an Approach to Confirm the Solubilization of CaHPO4 by Microbial Strains Using Glycine max as a Model

et al. 2021

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The sustainable development of agriculture can be stimulated by the great market availability of bio-inputs, including phosphate-solubilizing microbial strains. However, these strains are currently selected using imprecise and questionable solubilization methodologies in solid or liquid media. We hypothesized that the hydroponic system could be a more efficient methodology for selecting phosphate-solubilizing strains as plant growth promoters. This methodology was tested using the plant Glycine max as a model. The growth-promoting potential of the strains was compared with that of the Biomaphos® commercial microbial mixture. The obtained calcium phosphate (CaHPO4) solubilization results using the hydroponic system were inconsistent with those observed in solid and liquid media. However, the tests in liquid medium demonstrated poor performances of Codinaeopsis sp. (328EF) and Hamigera insecticola (33EF) in reducing pH and solubilizing CaHPO4, which corroborates with the effects of biotic stress observed in G. max plants inoculated with these strains. Nevertheless, the hydroponic system allowed the characterization of Paenibacillus alvei (PA12), which is also efficient in solubilization in a liquid medium. The bacterium Lysinibacillus fusiformis (PA26) was the most effective in CaHPO4 solubilization owing to the higher phosphorus (P) absorption, growth promotion, and physiological performance observed in plants inoculated with this bacterium. The hydroponic method proved to be superior in selecting solubilizing strains, allowing the assessment of multiple patterns, such as nutritional level, growth, photosynthetic performance, and anatomical variation in plants, and even the detection of biotic stress responses to inoculation, obtaining strains with higher growth promotion potential than Biomaphos®. This study proposed a new approach to confirm the solubilizing activity of microorganisms previously selected in vitro and potentially intended for the bio-input market that are useful in P availability for important crops, such as soybeans.

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Isolation and Characterization of Thermo-tolerant Phosphate-Solubilizing Bacteria From a Phosphate Mine and Their Rock Phosphate Solubilizing Abilities

Cited by 19 publications

References 28 publications

Isolation, Identification and Screening of Manganese Solubilizing Fungi From Low-Grade Manganese Ore Deposits

Isolation, Identification and Screening of Manganese Solubilizing Fungi From Low-Grade Manganese Ore Deposits

Growth promotion ability of phosphate‐solubilizing bacteria from the soybean rhizosphere under maize–soybean intercropping systems

Efficiency of the Hydroponic System as an Approach to Confirm the Solubilization of CaHPO4 by Microbial Strains Using Glycine max as a Model

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