Isolation and Characterization of the Betaine Aldehyde Dehydrogenase Gene in

Li, Jun; Zeng, Han; Xue, Li; Xu, Lixin; Wang, Yingbo; Tang, Wei; Han, Liebao

doi:10.2174/1874070701004010018

Cited by 7 publications

(5 citation statements)

References 29 publications

(38 reference statements)

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“…Total RNA was extracted using TRIzol reagent (Invitrogen, Carlsbad, CA, USA). Then, 10 μg of total RNA from each sample of Arabidopsis and tobacco was transferred onto nylon film via high-salt (20× SSC: 3 M NaCl, 0.3 M sodium citrate, pH 7.0) solution after electrophoresis in 1% agarose gel and denatured with 6% formaldehyde [ 36 ]. Northern blotting was performed using full-length AgRPS3aE cDNA labeled with DIG-dUTP as a probe according to the protocol provided with the DIG-High Prime DNA Labeling and Detection Starter Kit I (Roche, Mannheim, Germany) [ 37 ].…”

Section: Methodsmentioning

confidence: 99%

A Ribosomal Protein AgRPS3aE from Halophilic Aspergillus glaucus Confers Salt Tolerance in Heterologous Organisms

Liang

Liu

Xie

et al. 2015

IJMS

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High salt in soils is one of the abiotic stresses that significantly reduces crop yield, although saline lands are considered potential resources arable for agriculture. Currently, genetic engineering for enhancing salt tolerance is being tested as an efficient and viable strategy for crop improvement. We previously characterized a large subunit of the ribosomal protein RPL44, which is involved in osmotic stress in the extremely halophilic fungus Aspergillus glaucus. Here, we screened another ribosomal protein (AgRPS3aE) that also produced high-salt tolerance in yeast. Bioinformatics analysis indicated that AgRPS3aE encodes a 29.2 kDa small subunit of a ribosomal protein belonging to the RPS3Ae family in eukaryotes. To further confirm its protective function against salinity, we expressed AgRPS3aE in three heterologous systems, the filamentous fungus Magnaporthe oryzae and two model plants Arabidopsis and tobacco. Overexpression of AgRPS3aE in all tested transformants significantly alleviated stress symptoms compared with controls, suggesting that AgRPS3aE functions not only in fungi but also in plants. Considering that ribosomal proteins are housekeeping components in organisms from prokaryotes to eukaryotes, we propose that AgRPS3aE is one of the optimal genes for improving high-salt tolerance in crops.

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Section: Methodsmentioning

confidence: 99%

A Ribosomal Protein AgRPS3aE from Halophilic Aspergillus glaucus Confers Salt Tolerance in Heterologous Organisms

Liang

Liu

Xie

et al. 2015

IJMS

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“…Interestingly, O. japonicus, a monocot, shows a pattern similar to the dicot accumulators. Although native GB accumulation data for O. japonicus is not reported, expression of O. japonicus CMO in tobacco led to 2-2.5 fold increase in GB accumulation (Liu et al 2010), suggestive of this monocot being an accumulator with a functional CMO.…”

Section: Discussionmentioning

confidence: 97%

Glycine betaine biosynthesis in saltbushes (Atriplex spp.) under salinity stress

2013

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Soil salinity and drought severely affect all aspects of plant physiology, leading to significant losses of crop productivity and native biodiversity. A key to sustainable land use in such areas is to cultivate well-adapted native plants that are also commercially important and have the appropriate gene pool. Glycine betaine (GB) is an osmoprotectant that imparts salt and drought tolerance to some plants. It is also shown separately to provide significant health benefits to animals and humans. We investigated whether Australian saltbushes, which are extremely salt and drought tolerant and also impart health benefits to grazing animals, may have the genetic basis for GB biosynthesis, explaining the two different observations. Complementary DNAs encoding the two key enzymes of the plant GB biosynthesis pathway, choline monooxygenase (CMO) and betaine aldehyde dehydrogenase (BADH), were identified and analysed from Atriplex nummularia and Atriplex semibaccata. The sequences showed the putative CMO proteins exhibited all functionally important features including the Reiske-type cluster (2Fe-2S) and mononuclear non-heme Fe cluster, and the putative BADHs exhibited conservation of active site residues. The expression of both genes was found to be significantly up-regulated in leaf tissues under salt stress. The leaf tissues also showed accumulation of very high levels of GB, at 29.69 mmol/kg fresh weight for A. nummularia and 42.68 mmol/kg fresh weight for A. semibaccata, which is several times higher than in cereal crops. The results demonstrate a strong potential of cultivation of saltbushes for re-vegetation and as a perennial fodder in salinity and drought-affected areas.

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“…It is synthesized through a two-step oxidation process from choline and betaine aldehyde, catalyzed by choline monooxygenase (CMO) and betaine aldehyde dehydrogenase (BADH), respectively [8]. GB stabilizes membranes, protects proteins and photosystem II, and mitigates oxidative damage [34] while the plant is under dehydrated stress or prevents cell damage when exposed to stress conditions such as drought, salinity, and low-temperature environments [35][36][37][38]. Similar to spinach, the CMO gene in oil palm is likely involved in glycine betaine biosynthesis and stress response.…”

Section: Discussionmentioning

confidence: 99%

Monitoring Drought Tolerance in Oil Palm: Choline Monooxygenase as a Novel Molecular Marker

Suraninpong,

Thongkhao,

Azzeme

et al. 2023

Plants

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Water scarcity negatively impacts oil palm production, necessitating the development of drought-tolerant varieties. This study aimed to develop molecular markers for oil palm breeding programs focused on drought tolerance. Genes associated with drought tolerance were selected, and single nucleotide polymorphism (SNP)-based markers were developed. Genomic DNA was successfully extracted from 17 oil palm varieties, and 20 primers out of 44 were effectively amplified. Screening with single-strand conformation polymorphism (SSCP) revealed an informative SNP marker from the choline monooxygenase (CMO) gene, exhibiting CC, CT, and TT genotypes. Notably, the oil palm variety La Mé showed the CT genotype, while Surat Thani 2 (Deli × La Mé) exhibited the CT and CC genotypes in a 1:1 ratio. Gene expression analysis confirmed the association of the CMO gene with drought tolerance in commercial oil palm varieties. The full-length CMO gene was 1308 bp long and shared sequence similarities with other plant species. However, amino acid sequence variations were observed compared with existing databases. These findings highlight the potential utility of the CMO marker for drought tolerance selection, specifically within the La Mé parent of oil palm Surat Thani 2 varieties, and strongly confirm the La Mé S5 population and Surat Thani 2 as drought-tolerant varieties.

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Isolation and Characterization of the Betaine Aldehyde Dehydrogenase Gene in

Cited by 7 publications

References 29 publications

A Ribosomal Protein AgRPS3aE from Halophilic Aspergillus glaucus Confers Salt Tolerance in Heterologous Organisms

A Ribosomal Protein AgRPS3aE from Halophilic Aspergillus glaucus Confers Salt Tolerance in Heterologous Organisms

Glycine betaine biosynthesis in saltbushes (Atriplex spp.) under salinity stress

Monitoring Drought Tolerance in Oil Palm: Choline Monooxygenase as a Novel Molecular Marker

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