A Ribosomal Protein AgRPS3aE from Halophilic  Aspergillus glaucus Confers Salt Tolerance in  Heterologous Organisms

Liang, Xilong; Liu, Yiling; Xie, Lixia; Liu, Xiaodan; Yi, Wei; Zhou, Xin; Zhang, Shihong

doi:10.3390/ijms16023058

Cited by 29 publications

(13 citation statements)

References 39 publications

(39 reference statements)

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“…Soybean RPS3a has been associated with disease resistance and flooding tolerance53. A recent study suggests that RPS3aE might improve salt tolerance in three heterologous organisms54. In the present study, RPS3aE was up-regulated both at 6 and 24 h. Thus, RPS3aE is a multifunctional protein that plays extra-ribosomal roles in the stress response to benzothiazole.…”

Section: Discussionsupporting

confidence: 58%

Proteomic profile of the Bradysia odoriphaga in response to the microbial secondary metabolite benzothiazole

Zhao

Cui

et al. 2016

Sci Rep

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Benzothiazole, a microbial secondary metabolite, has been demonstrated to possess fumigant activity against Sclerotinia sclerotiorum, Ditylenchus destructor and Bradysia odoriphaga. However, to facilitate the development of novel microbial pesticides, the mode of action of benzothiazole needs to be elucidated. Here, we employed iTRAQ-based quantitative proteomics analysis to investigate the effects of benzothiazole on the proteomic expression of B. odoriphaga. In response to benzothiazole, 92 of 863 identified proteins in B. odoriphaga exhibited altered levels of expression, among which 14 proteins were related to the action mechanism of benzothiazole, 11 proteins were involved in stress responses, and 67 proteins were associated with the adaptation of B. odoriphaga to benzothiazole. Further bioinformatics analysis indicated that the reduction in energy metabolism, inhibition of the detoxification process and interference with DNA and RNA synthesis were potentially associated with the mode of action of benzothiazole. The myosin heavy chain, succinyl-CoA synthetase and Ca+-transporting ATPase proteins may be related to the stress response. Increased expression of proteins involved in carbohydrate metabolism, energy production and conversion pathways was responsible for the adaptive response of B. odoriphaga. The results of this study provide novel insight into the molecular mechanisms of benzothiazole at a large-scale translation level and will facilitate the elucidation of the mechanism of action of benzothiazole.

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Section: Discussionsupporting

confidence: 58%

Proteomic profile of the Bradysia odoriphaga in response to the microbial secondary metabolite benzothiazole

Zhao

Cui

et al. 2016

Sci Rep

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“…The recombinant plasmid pTEV7:: CpLEA5 was introduced into Agrobacteriumtumefaciens (LBA4404). The transformation into Arabidopsis was carry out by using floral dip method [ 46 , 47 ]. CpLEA5 transgenic-seedlings were selected by MS medium containing kanamycin (40 mg/L).…”

Section: Methodsmentioning

confidence: 99%

CpLEA5, the Late Embryogenesis Abundant Protein Gene from Chimonanthus praecox, Possesses Low Temperature and Osmotic Resistances in Prokaryote and Eukaryotes

Liu

Xie

Liang

et al. 2015

IJMS

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Plants synthesize and accumulate a series of stress-resistance proteins to protect normal physiological activities under adverse conditions. Chimonanthus praecox which blooms in freezing weather accumulates late embryogenesis abundant proteins (LEAs) in flowers, but C. praecox LEAs are little reported. Here, we report a group of five LEA genes of C. praecox (CpLEA5, KT727031). Prokaryotic-expressed CpLEA5 was employed in Escherichia coli to investigate bioactivities and membrane permeability at low-temperature. In comparison with the vacant strains, CpLEA5-containing strains survived in a 20% higher rate; and the degree of cell membrane damage in CpLEA5-containing strains was 55% of that of the vacant strains according to a conductivity test, revealing the low-temperature resistance of CpLEA5 in bacteria. CpLEA5 was also expressed in Pichia pastoris. Interestingly, besides low-temperature resistance, CpLEA5 conferred high resistance to salt and alkali in CpLEA5 overexpressing yeast. The CpLEA5 gene was transferred into Arabidopsis thaliana to also demonstrate CpLEA5 actions in plants. As expected, the transgenic lines were more resistant against low-temperature and drought while compared with the wild type. Taken together, CpLEA5-conferred resistances to several conditions in prokaryote and eukaryotes could have great value as a genetic technology to enhance osmotic stress and low-temperature tolerance.

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“…Ribosomal proteins from the extremely halophilic fungus Aspergillus glaucus were also shown to alleviate salt stress when expressed in Arabidopsis thaliana and Nicotiana tabacum (Liu et al 2014;Liang et al 2015).…”

Section: Exploring Halobiomes As a Pool Of Genes For The Productionmentioning

confidence: 99%

Engineering salinity tolerance in plants: progress and prospects

Wani

Kumar

Khare

et al. 2020

Planta

154

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Soil salinity exerts significant constraints on global crop production, posing a serious challenge for plant breeders and biotechnologists. The classical transgenic approach for enhancing salinity tolerance in plants revolves by boosting endogenous defence mechanisms, often via a single gene 2 approach, and usually involves the enhanced synthesis of compatible osmolytes, antioxidants, polyamines, maintenance of hormone homeostasis, modification of transporters and/or regulatory proteins, including transcription factors (TFs) and alternative splicing events. Occasionally, genetic manipulation of regulatory proteins or phytohormone levels confers salinity-tolerance, but all these may cause undesired reduction in plant growth and/or yields. In this review, we present and evaluate novel and cutting-edge approaches for engineering salt tolerance in crop plants. First, we cover recent findings regarding the importance of regulatory proteins and transporters, and how they can be used to enhance salt tolerance in crop plants. We also evaluate the importance of halobiomes as a reservoir of genes that can be used for engineering salt-tolerance in glycophytic crops. Additionally, the role of microRNAs as critical post-transcriptional regulators in plant adaptive responses to salt stress are reviewed and their use for engineering salt-tolerant crop plants is critically assessed. The potentials of alternative splicing mechanisms and targeted gene-editing technologies in understanding plant salt-stress responses and developing salt-tolerant crop plants is also discussed.

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A Ribosomal Protein AgRPS3aE from Halophilic Aspergillus glaucus Confers Salt Tolerance in Heterologous Organisms

Cited by 29 publications

References 39 publications

Proteomic profile of the Bradysia odoriphaga in response to the microbial secondary metabolite benzothiazole

Proteomic profile of the Bradysia odoriphaga in response to the microbial secondary metabolite benzothiazole

CpLEA5, the Late Embryogenesis Abundant Protein Gene from Chimonanthus praecox, Possesses Low Temperature and Osmotic Resistances in Prokaryote and Eukaryotes

Engineering salinity tolerance in plants: progress and prospects

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