CpLEA5, the Late Embryogenesis Abundant Protein Gene from Chimonanthus praecox, Possesses Low Temperature and Osmotic Resistances in Prokaryote and Eukaryotes

Abstract

Plants synthesize and accumulate a series of stress-resistance proteins to protect normal physiological activities under adverse conditions. Chimonanthus praecox which blooms in freezing weather accumulates late embryogenesis abundant proteins (LEAs) in flowers, but C. praecox LEAs are little reported. Here, we report a group of five LEA genes of C. praecox (CpLEA5, KT727031). Prokaryotic-expressed CpLEA5 was employed in Escherichia coli to investigate bioactivities and membrane permeability at low-temperature. In comparison with the vacant strains, CpLEA5-containing strains survived in a 20% higher rate; and the degree of cell membrane damage in CpLEA5-containing strains was 55% of that of the vacant strains according to a conductivity test, revealing the low-temperature resistance of CpLEA5 in bacteria. CpLEA5 was also expressed in Pichia pastoris. Interestingly, besides low-temperature resistance, CpLEA5 conferred high resistance to salt and alkali in CpLEA5 overexpressing yeast. The CpLEA5 gene was transferred into Arabidopsis thaliana to also demonstrate CpLEA5 actions in plants. As expected, the transgenic lines were more resistant against low-temperature and drought while compared with the wild type. Taken together, CpLEA5-conferred resistances to several conditions in prokaryote and eukaryotes could have great value as a genetic technology to enhance osmotic stress and low-temperature tolerance.

Transcriptomes Analysis Reveals Novel Insight Into the Molecular Mechanisms of Somatic Embryogenesis in Hevea Brasiliensis

“…Ca 2+ has been identi ed to play a mediating role during plant SE [37,101]. LEA5, a late embryogenesis abundant proteins gene, was highly expressed in late embryogenesis [102]. In our study, SERK1, CML13, CML36 and CAM-7 were up-regulated in CE.…”

Global scale transcriptome analysis reveals differentially expressed genes involve in early somatic embryogenesis in Dimocarpus longan Lour

“…LEA5 belonged to the fifth group of late embryogenesis abundant proteins gene, were abundant in late embryogenesis of mature seed, and involved in the abiotic stresses responses [119]. CNOT3 , might be a new CCR4-NOT complex gene in plant, which had proved in regulation of cell division in HeLa cell, while its functions on plant was poorly-understood.…”

Physical Map of FISH 5S rDNA and (AG3T3)3 Signals Displays Chimonanthus campanulatus R.H. Chang & C.S. Ding Chromosomes, Reproduces its Metaphase Dynamics and Distinguishes Its Chromosomes

“…Studies on Ch. praecox have focused on transcriptomic and proteomic profiling throughout flower development [13,14,15,16]; fragrance gene identification [17]; floral scent emission from nectaries on the adaxial side of the innermost and middle petals [18]; separation and determination of volatile compounds [19,20], phenolic compounds [21], alkaloids and flavonoids [22,23,24], and sesquiterpenoids [25]; in vitro culture system development [26]; genetic linkage map construction [27]; simple sequence repeat (SSR) [28], expressed sequence tag (EST) [29], and amplified fragment length polymorphism (AFLP) [30] development; and ANL2 [31], CpAGL2 [32], CpAP3 [33], CpCAF1 [34], CpCZF1/2 [35], Cpcor413pm1 [36], CpEXP1 [37], CpH3 [38], CpLEA5 [39], Cplectin [29], CpNAC8 [40], CpRALF [41], CpRBL [42], FPPS [43], and G6PDH1 [44] cloning and development. Studies on Ch.…”