Isolation and characterization of the three Waxy genes encoding the granule-bound starch synthase in hexaploid wheat

Murai, James T.; Taira, Tomoaki; Ohta, Daisaku

doi:10.1016/s0378-1119(99)00178-x

Cited by 114 publications

(63 citation statements)

References 22 publications

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“…A high degree of conservation was found within the RPL3-family with similarities among homoeologous genes that are slightly higher than reported from studies with other genes from wheat: similarity among the RPL3 homoeologs lies between 97.3 and 98.7%, while cDNA sequences of the Waxy granule-bound starch synthase homoeologs are about 96% similar (Murai et al 1999), that of the wknox1 (orthologs of the KNOTTED1 homeobox protein from maize) about 95% (Takumi et al 2000), and PDI (protein disulWde isomerase) homoeologous genes show 96-97.5% identity (CiaY et al 2005).…”

Section: Discussionmentioning

confidence: 58%

Cloning and characterization of the ribosomal protein L3 (RPL3) gene family from Triticum aestivum

et al. 2007

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Plant pathogenic fungi of the genus Fusarium can cause severe diseases on small grain cereals and maize. The contamination of harvested grain with Fusarium mycotoxins is a threat to human and animal health. In wheat production of the toxin deoxynivalenol (DON), which inhibits eukaryotic protein biosynthesis, is a virulence factor of Fusarium, and resistance against DON is considered to be part of Fusarium resistance. Previously, single amino acid changes in RPL3 (ribosomal protein L3) conferring DON resistance have been described in yeast. The goal of this work was to characterize the RPL3 gene family from wheat and to investigate the potential role of naturally existing RPL3 alleles in DON resistance by comparing Fusarium-resistant and susceptible cultivars. The gene family consists of three homoeologous alleles of both RPL3A and RPL3B, which are located on chromosomes 4A

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Section: Discussionmentioning

confidence: 58%

Cloning and characterization of the ribosomal protein L3 (RPL3) gene family from Triticum aestivum

et al. 2007

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“…Based on nucleotide sequences of the wild-type and null waxy alleles 11,25 , primer sets capable of identifying the genotype at each waxy locus were designed 12 (Figs. 1 and 2).…”

Section: Pcr-based Markers For Each Null Allele Of Waxy Wheatmentioning

confidence: 99%

DNA Markers for Identifying waxy Mutations and Improving Noodle Quality in Wheat

Vrinten

Nakamura

2010

JARQ

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“…For example, in case of wheat, a STS marker for polyphenol oxidase (PPO) activity developed from the EST of the PPO gene, was found to discriminate accurately between 233 Chinese varieties with low and high PPO activity (Sun et al 2005). Gene specific markers for waxy starch were used to select the wheat materials in Australia that had the wx-B allele, which is associated with good Asian noodle quality (Murai et al 1999).…”

Section: Superiority Of Fms Over Traditional Markers In Masmentioning

confidence: 99%

Gene-Based Marker Systems in Plants: High Throughput Approaches for Marker Discovery and Genotyping

Varshney

2009

Molecular Techniques in Crop Improvement

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Development and application of molecular markers derived from genes, commonly called genic markers or sometimes functional markers, is gaining momentum in plant genetics and breeding. Availability of large amount of sequence data coming from genome/transcriptome sequencing projects as well as advent of next generation sequencing technologies together with advances in bioinformatics tools, marker discovery is becoming cheaper and faster. The availability of inexpensive high-density SNP-genotyping arrays is encouraging the plant genetics and breeding community to undertake genome-wide marker genotyping for a variety of applications. For instance, high-throughput and low cost genotyping assays for gene-based markers offers the possibility to accelerate the trait mapping based on high-density linkage mapping as well as genome-scanning based association mapping approaches in addition to facilitate physical mapping, comparative mapping, phylogenetic studies and understanding genome organization in crop plant species. Marker discovery, genotyping and molecular breeding practices would be routine in near future for crop improvement in many crop species. Advances in the area of marker discovery and genotyping using highly parallel genomics assays and also a few applications have been discussed in this chapter.

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Isolation and characterization of the three Waxy genes encoding the granule-bound starch synthase in hexaploid wheat

Cited by 114 publications

References 22 publications

Cloning and characterization of the ribosomal protein L3 (RPL3) gene family from Triticum aestivum

Cloning and characterization of the ribosomal protein L3 (RPL3) gene family from Triticum aestivum

DNA Markers for Identifying waxy Mutations and Improving Noodle Quality in Wheat

Gene-Based Marker Systems in Plants: High Throughput Approaches for Marker Discovery and Genotyping

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