Cloning and characterization of the ribosomal protein L3 (RPL3) gene family from Triticum aestivum

Lucyshyn, Doris; Busch, B.; Abolmaali, Shamsozoha; Steiner, Barbara; Chandler, E.; Sanjarian, Forough; Mousavi, Amir; Nicholson, P.; Buerstmayr, Hermann; Adam, Gerhard

doi:10.1007/s00438-006-0201-1

Cited by 18 publications

(7 citation statements)

References 37 publications

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“…Asparagine synthetase is also involved in responding to various factors, such as pathogen infection and oxidative stress [ 64 ]. In wheat, deoxynivalenol (DON) is a virulence factor of Fusarium , and resistance against DON is considered to be part of Fusarium resistance [ 65 ]. The evidence for resistance to DON by ribosomal protein L3 ( RPL3 ) was claimed by Miller and Ewen [ 66 ] and Miller and Arnison [ 67 ].…”

Section: Discussionmentioning

confidence: 99%

“…In wheat, six RPL3 genes, three homologous versions of both paralogs, RPL3-A and RPL3-B , were expected to be found in T . aestivum [ 65 ]. In our present work, two AS (W5CK94 and W5FDU3) and two RPL3 (RPL3, A1YE31 and A1YE34) were up-regulated during artificial ageing, indicating that the protection system was triggered in wheat seeds to avoid pathogen penetration.…”

Section: Discussionmentioning

confidence: 99%

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Quantitative Proteomic Analysis of Wheat Seeds during Artificial Ageing and Priming Using the Isobaric Tandem Mass Tag Labeling

Zhang

Wang

et al. 2016

PLoS ONE

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Wheat (Triticum aestivum L.) is an important crop worldwide. The physiological deterioration of seeds during storage and seed priming is closely associated with germination, and thus contributes to plant growth and subsequent grain yields. In this study, wheat seeds during different stages of artificial ageing (45°C; 50% relative humidity; 98%, 50%, 20%, and 1% Germination rates) and priming (hydro-priming treatment) were subjected to proteomics analysis through a proteomic approach based on the isobaric tandem mass tag labeling. A total of 162 differentially expressed proteins (DEPs) mainly involved in metabolism, energy supply, and defense/stress responses, were identified during artificial ageing and thus validated previous physiological and biochemical studies. These DEPs indicated that the inability to protect against ageing leads to the incremental decomposition of the stored substance, impairment of metabolism and energy supply, and ultimately resulted in seed deterioration. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that the up-regulated proteins involved in seed ageing were mainly enriched in ribosome, whereas the down-regulated proteins were mainly accumulated in energy supply (starch and sucrose metabolism) and stress defense (ascorbate and aldarate metabolism). Proteins, including hemoglobin 1, oleosin, agglutinin, and non-specific lipid-transfer proteins, were first identified in aged seeds and might be regarded as new markers of seed deterioration. Of the identified proteins, 531 DEPs were recognized during seed priming compared with unprimed seeds. In contrast to the up-regulated DEPs in seed ageing, several up-regulated DEPs in priming were involved in energy supply (tricarboxylic acid cycle, glycolysis, and fatty acid oxidation), anabolism (amino acids, and fatty acid synthesis), and cell growth/division. KEGG and protein-protein interaction analysis indicated that the up-regulated proteins in seed priming were mainly enriched in amino acid synthesis, stress defense (plant-pathogen interactions, and ascorbate and aldarate metabolism), and energy supply (oxidative phosphorylation and carbon metabolism). Therefore, DEPs associated with seed ageing and priming can be used to characterize seed vigor and optimize germination enhancement treatments. This work reveals new proteomic insights into protein changes that occur during seed deterioration and priming.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Quantitative Proteomic Analysis of Wheat Seeds during Artificial Ageing and Priming Using the Isobaric Tandem Mass Tag Labeling

Zhang

Wang

et al. 2016

PLoS ONE

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“…As such, we have proposed a novel mechanism for the interaction of T-2 toxin with the eukaryotic ribosome, which ideally, will be applicable to other toxins of this family. Undoubtedly, this new information is a stepping stone towards the guided development of trichothecene resistance genes which can be developed for the introduction into transgenic plants, such as those previously detected by groups such as Mitterbauer et al for the engineering of Triticale cultivars [61]. The development of trichothecene resistance genes is essential to the prevention of crop loss due to Fusarium Head Blight and root rot, and will help to circumvent the occurrence of alimentary toxic aleukia, and other related ailments.…”

Section: Discussionmentioning

confidence: 99%

Hydrogen-Bonding Interactions in T-2 Toxin Studied Using Solution and Solid-State NMR

Chaudhary

Shank

Montina

et al. 2011

Toxins

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The structure of T-2 toxin in the solid-state is limited to X-ray crystallographic studies, which lack sufficient resolution to provide direct evidence for hydrogen-bonding interactions. Furthermore, its solution-structure, despite extensive Nuclear Magnetic Resonance (NMR) studies, has provided little insight into its hydrogen-bonding behavior, thus far. Hydrogen-bonding interactions are often an important part of biological activity. In order to study these interactions, the structure of T-2 toxin was compared in both the solution- and solid-state using NMR Spectroscopy. It was determined that the solution- and solid-state structure differ dramatically, as indicated by differences in their carbon chemical shifts, these observations are further supported by solution proton spectral parameters and exchange behavior. The slow chemical exchange process and cross-relaxation dynamics with water observed between the hydroxyl hydrogen on C-3 and water supports the existence of a preferential hydrogen bonding interaction on the opposite side of the molecule from the epoxide ring, which is known to be essential for trichothecene toxicity. This result implies that these hydrogen-bonding interactions could play an important role in the biological function of T-2 toxin and posits towards a possible interaction for the trichothecene class of toxins and the ribosome. These findings clearly illustrate the importance of utilizing solid-state NMR for the study of biological compounds, and suggest that a more detailed study of this whole class of toxins, namely trichothecenes, should be pursued using this methodology.

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“…In addition to these two main types of FHB resistance, there is resistance to kernel infection (Type III), host tolerance to FHB and/or DON (Type IV), and resistance to the accumulation of DON (Type V) ( Boutigny et al , 2008 ; Gunupuru et al , 2017 ). Single amino acid changes to the DON target, ribosomal protein L3 (RPL3), have been demonstrated to improve tolerance to DON in yeast, and hence this is a possible target to improve type IV resistance ( Mitterbauer et al , 2004 ; Lucyshyn et al , 2007 ). Type V resistance is commonly considered to be a component of Type II resistance, as it typically limits disease spread ( Gunupuru et al , 2017 ), and can be subdivided into Class 1, processes that chemically modify DON to a less toxic form; and Class 2, processes that prevent the accumulation of DON and other trichothecene mycotoxins ( Boutigny et al , 2008 ).…”

Section: Introductionmentioning

confidence: 99%

Type II Fusarium head blight susceptibility conferred by a region on wheat chromosome 4D

Hales

Steed

Giovannelli

et al. 2020

Journal of Experimental Botany

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Fusarium head blight (FHB) causes significant grain yield and quality reductions in wheat and barley. Most wheat varieties are incapable of preventing FHB spread through the rachis, but disease is typically limited to individually infected spikelets in barley. We point-inoculated wheat lines possessing barley chromosome introgressions to test whether FHB resistance could be observed in a wheat genetic background. The most striking differential was between 4H(4D) substitution and 4H addition lines. The 4H addition line was similarly susceptible to the wheat parent, but the 4H(4D) substitution line was highly resistant, which suggests that there is an FHB susceptibility factor on wheat chromosome 4D. Point inoculation of Chinese Spring 4D ditelosomic lines demonstrated that removing 4DS results in high FHB resistance. We genotyped four Chinese Spring 4DS terminal deletion lines to better characterize the deletions in each line. FHB phenotyping indicated that lines del4DS-2 and del4DS-4, containing smaller deletions, were susceptible and had retained the susceptibility factor. Lines del4DS-3 and del4DS-1 contain larger deletions and were both significantly more resistant, and hence had presumably lost the susceptibility factor. Combining the genotyping and phenotyping results allowed us to refine the susceptibility factor to a 31.7 Mbp interval on 4DS.

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Cloning and characterization of the ribosomal protein L3 (RPL3) gene family from Triticum aestivum

Cited by 18 publications

References 37 publications

Quantitative Proteomic Analysis of Wheat Seeds during Artificial Ageing and Priming Using the Isobaric Tandem Mass Tag Labeling

Quantitative Proteomic Analysis of Wheat Seeds during Artificial Ageing and Priming Using the Isobaric Tandem Mass Tag Labeling

Hydrogen-Bonding Interactions in T-2 Toxin Studied Using Solution and Solid-State NMR

Type II Fusarium head blight susceptibility conferred by a region on wheat chromosome 4D

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