Isolation and characterization of the distal promoter region of mouse Cbfa1

Fujiwara, Masanori; Tagashira, Shuzo; Harada, Hideyuki; Ogawa, S.; Katsumata, Takashi; Nakatsuka, Masashi; Komori, Toshihisa; Takada, Hiroshi

doi:10.1016/s0167-4781(99)00113-x

Cited by 41 publications

(39 citation statements)

References 33 publications

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“…Earlier studies demonstrated that runt domain in Cbfa1 gene could bind to osteoblast-specific cis-acting element 2 (OSE2) in the promoter of the rat BGP gene, thereby Cbfa1 regulated the expression of BGP [18]. The expressions of these genes might be induced by Cbfa1, but a Cbfa1-specific antisense oligonucleotide could block Cbfa1-induced bone anabolic activity [6,19].…”

Section: Discussionmentioning

confidence: 99%

Regulation of Cbfa1 Expression by Total Flavonoids of Herba Epimedii

et al. 2006

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Abstract. Core binding factor a1 (Cbfa1) is a member of the runt family of transcription factors, which appears to play a pivotal role in regulating the differentiation of osteoblastic precursors and the activity of mature osteoblasts. Total flavonoids of Herba epimedii (HEF) is a recognized bone anabolic agent, but there is lack of reports on the modulation of Cbfa1 expression by HEF. Here we investigated the effect of HEF on Cbfa1 expression in the bone of ovariectomized (OVX) rats. HEF could increase the expression of Cbfa1 mRNA in the bone of ovariectomized rats in a dose-dependent manner. Furthermore, the high dose HEF (160 mg/kg) administered for 12 weeks in vivo stimulated osteocalcin expression. These findings suggest that Cbfa1 is required for mediating the anabolic effects of HEF.

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Section: Discussionmentioning

confidence: 99%

Regulation of Cbfa1 Expression by Total Flavonoids of Herba Epimedii

et al. 2006

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“…Functional analyses using transfection assays have demonstrated that the P1 and P2 regions of the three genes possess promoter activity Fujiwara et al, 1999;Drissi et al, 2000;Bangsow et al, 2001;Rini and Calabi, 2001;Xiao et al, 2001;Zambotti et al, 2002). In most cases, the activity was cell-type specific, with higher promoter-reporter readouts in cells that normally express the genes.…”

Section: Tissue-specific Transcription Control Of Runx Genesmentioning

confidence: 99%

“…The structural similarities between the mammalian RUNX include genomic elements that are involved in expression regulation. Each of the three genes is transcriptionally regulated by two distantly located promoter regions, P1 (distal) and P2 (proximal) Stewart et al, 1997;Fujiwara et al, 1999;Drissi et al, 2000;Bangsow et al, 2001;Rini and Calabi, 2001;Telfer and Rothenberg, 2001;Xiao et al, 2001). The P2 promoter is nested within a particularly large CpG island (Bangsow et al, 2001; Levanon et al, 2001b) (Figure 1d), which is also conserved in Fugu (Eggers et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Structure and regulated expression of mammalian RUNX genes

2004

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The RUNX are key regulators of lineage-specific gene expression in major developmental pathways. The expression of RUNX genes is tightly regulated, leading to a highly specific spatio/temporal expression pattern and to distinct phenotypes of gene knockouts. This review highlights the extensive structural similarities between the three mammalian RUNX genes and delineates how regulation of their expression at the levels of transcription and translation are orchestrated into the unique RUNX expression pattern.

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“…An important conserved feature of the vertebrate Runx genes is their expression from two promoters (P1 and P2) that encode isoforms with distinct amino-terminal sequences (10 -12). Functional analyses using transfection assays have demonstrated that the P1 and P2 promoters both contribute to the expression of Runx genes (13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24). However, our knowledge of the positive and negative elements that regulate basal and tissue-specific expression of Runx genes is still incomplete.…”

mentioning

confidence: 99%

Co-stimulation of the Bone-related Runx2 P1 Promoter in Mesenchymal Cells by SP1 and ETS Transcription Factors at Polymorphic Purine-rich DNA Sequences (Y-repeats)

Zhang

Hassan

Xie

et al. 2009

Journal of Biological Chemistry

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Transcriptional control of Runx2 gene expression through two alternative promoters (P1 and P2) is critical for the execution of its function as an osteogenic cell fate determining factor. In all vertebrates examined to date, the bone related P1 promoter contains a purine-rich region (؊303 to ؊128 bp in the rat) that separates two regulatory domains. The length of this region differs dramatically between species even within the same order. Using deletion analysis, we show that part of this purine-rich region (؊200 to ؊128) containing a duplicated element (Y-repeat) positively regulates Runx2 P1 transcription. Electrophoretic mobility assays and chromatin immunoprecipitations reveal that Y-repeat binds at least two different classes of transcription factors related to GC box binding proteins (e.g. SP1 and SP7/Osterix) and ETS-like factors (e.g. ETS1 and ELK1). Forced expression of SP1 increases Runx2 P1 promoter activity through the Y-repeats, and small interfering RNA depletion of SP1 decreases Runx2 expression. Similarly, exogenous expression of wild type ELK1, but not a defective mutant that cannot be phosphorylated, enhances Runx2 gene expression. SP1 is most abundant in proliferating cells, and ELK1 is most abundant in postconfluent cells; during MC3T3-E1 osteoblast differentiation, both proteins are transiently co-expressed when Runx2 expression is enhanced. Taken together, our data suggest that basal Runx2 gene transcription is regulated by dynamic interactions between SP1 and ETS-like factors during progression of osteogenesis.

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Isolation and characterization of the distal promoter region of mouse Cbfa1

Cited by 41 publications

References 33 publications

Regulation of Cbfa1 Expression by Total Flavonoids of Herba Epimedii

Regulation of Cbfa1 Expression by Total Flavonoids of Herba Epimedii

Structure and regulated expression of mammalian RUNX genes

Co-stimulation of the Bone-related Runx2 P1 Promoter in Mesenchymal Cells by SP1 and ETS Transcription Factors at Polymorphic Purine-rich DNA Sequences (Y-repeats)

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