Isolation and characterization of the immunoglobin of pike (Esox lucius L.)

Abstract

The purification of the immunoglobulin from pike serum and its physicochemical characterization is presented. The immunoglobulin was prepared by means of gel filtration and ion exchange chromatography. Measurements in the analytical ultracentrifuge showed a sedimentation constant of 15.0 S. A molecular weight of 650.000 was calculated. The immunoglobulin was composed of heavy and light chains of molecu]ar weights 60.000 and 22.500, respectively. It is likely that the immunoglobulin of pike is composed of 8 heavy and 8 light chains and possesses a tetrameric structure. The heavy chains contain 9.2% sugars and amino sugars. The amino acid composition of the chains is similar to that of other fish immunoglobulins.

Presence of antibodies specific for proteins ofContracaecum osculatum(Rudolphi, 1908) in plasma of several Antarctic teleosts

“…The L chain band, obtained by SDS-PAGE of C. hamatus Ig, seems to be double. This result, once further verified, would be in agreement with the data on the multiplicity of fish IgL chain reported by Clerx et al (1980) on Esox lucius, Warr (1983) on Spheroides glaber and Lobb et al (1984) on Ictalurus punctatus. The presence of an Ig monomeric form, as a minor component of the FPLC eluate cannot be excluded.…”

Immunoglobulin in fish—genes, expression and structure

“…70-72 22·5 (Clerx et al, 1980) Perch Perca fluviatilis tetramer 65-72 28 (Richter & Ambrosius, 1972;Whittington, 1993) Toadfish Spheroides glaber tetramer (Warr, 1983) Tench Tinca tinca 67·5 26·5 (Vilain et al, 1984) Gilthead seabream…”

Acquired immunity to amyloodiniosis is associated with an antibody response

“…A variety of techniques have been employed in attempts to isolate piscine Ig with varying results (Acton 1971, Bradshaw et al 1971, Clerx et al 1980, Lobb 1981a, b, Ourth 1986, Phillips & Ourth 1986, Havarstein et al 1988, Smith et al 1993a). Smith et al (1993a) injected fish with goat IgG without a n adjuvant to produce polyclonal anti-goat Ig in the fish.…”