Isolation and characterization of the first Chinese porcine sapelovirus strain

Lan, Daoliang; Ji, Wenhui; Yang, Shixing; Cui, Li; Zhou, Yang; Yuan, Chen; Hua, Xiuguo

doi:10.1007/s00705-011-1035-7

Cited by 68 publications

(111 citation statements)

References 29 publications

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“…The length of the 3′ UTR in PSV is 82 nucleotides (strain V13, YC2011, KS04105, KS05151 and KS055217) although a shorter sequence (68 nucleotides) was described for the csh strain [20]. In a previous report [12], PSV strain V13 was predicted to include three stem-loop structures in the region of the 3′UTR using nine nucleotides of the terminal part of the 3D coding region.…”

Section: Discussionmentioning

confidence: 99%

“…PSV infections have been associated with a wide spectrum of symptoms ranging from asymptomatic infection to clinical signs including diarrhea, pneumonia, polioencephalomyelitis, and reproductive disorders [19]–[20]. Although PSVs can be important pathogens because of their wide distribution and high prevalence [21]–[25], the near-complete genomic sequences of only three PSV strains have been reported previously; one from the U.K. (V13 strain) and two from China (csh and YC2011 strains).…”

Section: Introductionmentioning

confidence: 99%

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Full-Length Genomic Analysis of Korean Porcine Sapelovirus Strains

et al. 2014

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Porcine sapelovirus (PSV), a species of the genus Sapelovirus within the family Picornaviridae, is associated with diarrhea, pneumonia, severe neurological disorders, and reproductive failure in pigs. However, the structural features of the complete PSV genome remain largely unknown. To analyze the structural features of PSV genomes, the full-length nucleotide sequences of three Korean PSV strains were determined and analyzed using bioinformatic techniques in comparison with other known PSV strains. The Korean PSV genomes ranged from 7,542 to 7,566 nucleotides excluding the 3′ poly(A) tail, and showed the typical picornavirus genome organization; 5′untranslated region (UTR)-L-VP4-VP2-VP3-VP1-2A-2B-2C-3A-3B-3C-3D-3′UTR. Three distinct cis-active RNA elements, the internal ribosome entry site (IRES) in the 5′UTR, a cis-replication element (CRE) in the 2C coding region and 3′UTR were identified and their structures were predicted. Interestingly, the structural features of the CRE and 3′UTR were different between PSV strains. The availability of these first complete genome sequences for PSV strains will facilitate future investigations of the molecular pathogenesis and evolutionary characteristics of PSV.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Full-Length Genomic Analysis of Korean Porcine Sapelovirus Strains

et al. 2014

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“…The symptomatic disorders include diarrhoea, pneumonia, polioencephalomyelitis and reproductive disorders (Alexandersen et al , 2012; Huang et al , 1980; Lamont & Betts, 1960; Lan et al , 2011; Schock et al , 2014; Sibalin, 1963). Experimental studies have demonstrated a diverse range of clinical symptoms (Lamont & Betts, 1960; Lan et al , 2011; Sibalin, 1963; Yamanouchi et al , 1965). These differences largely depend on age, route of infection and strains inoculated (Alexander & Betts, 1967; Lamont & Betts, 1960; Lan et al , 2011; Sibalin, 1963; Yamanouchi et al , 1965).…”

Section: Introductionmentioning

confidence: 99%

Pathogenesis of Korean Sapelovirus A in piglets and chicks

Kim

Kang

Son

et al. 2016

Journal of General Virology

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Sapelovirus A (SV-A), formerly known as porcine sapelovirus as a member of a new genus Sapelovirus, is known to cause enteritis, pneumonia, polioencephalomyelitis and reproductive disorders in pigs. We have recently identified α2,3-linked sialic acid on GD1a ganglioside as a functional SV-A receptor rich in the cells of pigs and chickens. However, the role of GD1a in viral pathogenesis remains elusive. Here, we demonstrated that a Korean SV-A strain could induce diarrhoea and intestinal pathology in piglets but not in chicks. Moreover, this Korean SV-A strain had mild extra-intestinal tropisms appearing as mild, non-suppurative myelitis, encephalitis and pneumonia in piglets, but not in chicks. By real-time reverse transcription (RT) PCR, higher viral RNA levels were detected in faecal samples than in sera or extra-intestinal organs from virus-inoculated piglets. Immunohistochemistry confirmed that high viral antigens were detected in the epithelial cells of intestines from virus-inoculated piglets but not from chicks. This Korean SV-A strain could bind the cultured cell lines originated from various species, but replication occurred only in cells of porcine origin. These data indicated that this Korean SV-A strain could replicate and induce pathology in piglets but not in chicks, suggesting that additional porcine-specific factors are required for virus entry and replication. In addition, this Korean SV-A strain is enteropathogenic, but could spread to the bloodstream from the gut and disseminate to extra-intestinal organs and tissues. These results will contribute to our understanding of SV-A pathogenesis so that efficient anti-sapelovirus drugs and vaccines could be developed in the future.

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“…To date, the genus Sapelovirus consists of three species, porcine, simian, and avian (9). Porcine sapelovirus (PSV) is confirmed to replicate in the gastrointestinal epithelia that causes acute diarrhea, pneumonia, and polioencephalomyelitis in swine (3).…”

mentioning

confidence: 99%