Complete Genome Sequence of a Novel Porcine Sapelovirus Strain YC2011 Isolated from Piglets with Diarrhea

Chen, Feng; Zhou, Qingfeng; Li, Wei; Song, Yanhua; Pan, Ye; Zhang, Xiangbin; Xue, Chunyi; Bi, Yanlan; Cao, Yongchang

doi:10.1128/jvi.01799-12

Cited by 30 publications

(22 citation statements)

References 9 publications

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“…Unlike, porcine teschovirus (PTV) and PEV (Merkle et al., ) both of which were previously classified as members of the genus Enterovirus , PSV causes unique type II cytopathic effect in porcine kidney cells (Honda et al., ; Knowles, Buckley, & Pereira, ; Palmquist, Munir, Taku, Kapur, & Goyal, ). All three viruses can be found in the faeces (Cano‐Gomez, Garcia‐Casado, Soriguer, Palero, & Jimenez‐Clavero, ; Chen et al., ) making aseptic sample collection of neurologic tissue imperative for accurate diagnosis.…”

Section: Introductionmentioning

confidence: 99%

Detection of a novel sapelovirus in central nervous tissue of pigs with polioencephalomyelitis in the USA

Arruda

Schwartz

et al. 2017

Transbound Emerg Dis

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An approximately 3,000 finishing swine operation in the United States experienced an outbreak of an atypical neurologic disease in 11-weeks-old pigs with an overall morbidity of 20% and case fatality rate of 30%. The clinical onset and progression of signs in affected pigs varied but included inappetence, compromised ambulation, ataxia, incoordination, mental dullness, paresis, paralysis and decreased response to environmental stimuli. Tissues from affected pigs were submitted for diagnostic investigation. Histopathologic examination of the cerebrum, cerebellum and spinal cord revealed severe lymphoplasmacytic and necrotizing polioencephalomyelitis with multifocal areas of gliosis and neuron satellitosis, suggestive of a neurotropic viral infection. Bacterial pathogens were not isolated by culture of neurologic tissue from affected pigs. Samples tested by polymerase chain reaction (PCR) were negative for pseudorabies virus and atypical porcine pestivirus. Immunohistochemistry for porcine reproductive and respiratory syndrome virus, porcine circovirus and Listeria was negative. Porcine sapelovirus (PSV) was identified in spinal cord by a nested PCR used to detect porcine enterovirus, porcine teschovirus and PSV. Next-generation sequencing of brainstem and spinal cord samples identified PSV and the absence of other or novel pathogens. In addition, Sapelovirus A mRNA was detected in neurons and nerve roots of the spinal cord by in situ hybridization. The PSV is genetically novel with an overall 94% amino acid identity and 86% nucleotide identity to a recently reported sapelovirus from Korea. This is the first case report in the United States associating sapelovirus with severe polioencephalomyelitis in pigs.

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Section: Introductionmentioning

confidence: 99%

Detection of a novel sapelovirus in central nervous tissue of pigs with polioencephalomyelitis in the USA

Arruda

Schwartz

et al. 2017

Transbound Emerg Dis

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“…This genus comprised three species, including Porcine Sapelovirus (PSV) (Fauquet et al, 2005), Simian Sapelovirus and Avian Sapelovirus. Porcine Sapelovirus 1 formally named Porcine Enterovirus 8 (PEV-8) and classified as a Porcine Enterovirus A (PEV A), Porcine Sapelovirus strain csh and Porcine Sapelovirus strain YC2011 are the three well acknowledged strains of PSV (Chen et al, 2012;Krumbholz et al, 2002;Lan et al, 2011). The genome of PSV is a single-stranded positive sense RNA with length of 7.5-8.3 kb nucleotides (Jauka, 2010).…”

Section: Introductionmentioning

confidence: 99%

Rapid and real-time detection of Porcine Sapelovirus by reverse transcription loop-mediated isothermal amplification assay

Wang

Cui

et al. 2014

Journal of Virological Methods

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The present study describes the development and validation of a one-step, single-tube, and real-time reverse transcription loop-mediated isothermal amplification (RT-LAMP) detecting Porcine Sapelovirus. RT-LAMP characterized by one strand displacement reaction with the specific stem-loop structure and Bst DNA polymerase could be finished in 60 min under isothermal condition at 63 °C. RT-LAMP assay showed higher sensitivity with 10(1) copies/μL than RT-PCR for the detection of Sapelovirus. The specificity of RT-LAMP assay was validated by the absence of any cross-reaction with other closely related virus in Picornaviridae group and other common virus causing porcine diarrhea. 7 positive Sapelovirus infection out of 63 fecal samples were identified using RT-LAMP, while 5 positive samples were determined by a conventional RT-PCR. A cost-effective method for Saplovirus detection with high sensitivity and specificity was developed and evaluated.

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“…PSV (strain csh) was first isolated from China in 2011 (Lan et al 2011). As on date, 50 complete genomic sequences of PSV strains have been published from China, South Korea, Japan, Germany, France, India and the United Kingdom (Lan et al 2011;Chen et al 2012;Schock et al 2014;Son et al 2014;Ray et al 2018;Piorkowski et al 2018;Bai et al 2018). Different virus strains exhibit affinity towards different target organs, as the PSV-csh strain (China, Shanghai) and PSV-G5 (the United Kingdom) affect mostly nervous system (Lan et al 2011;Schock et al 2014), whereas strains like KS0515, KS04105 and KS0552179 and Chinese PSV strain YC2011 are associated with diarrhoea.…”

Section: Virus Taxonomymentioning

confidence: 99%

Sapelovirus

Malik¹,

Bhat²,

Vlasova³

et al. 2020

Emerging and Transboundary Animal Viruses

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Sapelovirus (SPV), an emerging virus in the family Picornaviridae, is detected in several animal and bird species irrespective of their age. Amid all SPVs, porcine sapeloviruses (PSVs) are more ubiquitously present all over the world in porcine population. These viruses are highly stable in different environmental conditions and spread easily within the susceptible animals mainly through faeco-oral route. Usually, PSVs cause asymptomatic infections but are also clinically associated with encephalomyelitis, respiratory distress, fertility disorders and skin lesions. PSV-associated outbreaks have been reported where death occurs due to polioencephalitis and respiratory paralysis. Till date, PSVs have been detected from several European and Asian countries with moderate-to-high prevalence and clinical course. Viral capsid proteins are immunogenic and mutations in these pro-346 teins are responsible for the diverse nature of the viruses. Further, genomic analysis shows the varied evolutionary patterns and the presence of recombination within PSV strains. These viruses also exist as concurrent infections with several enteric bacterial, viral and parasitic pathogens. Classical to modern biotechnological assays are in use to detect PSV involving virus isolation in cell culture, immunohistochemistry, conventional nucleic acid amplification techniques, quantitative real-time amplification assays and isothermal amplification molecular techniques. Till date, there is no vaccine available against PSVs.

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Complete Genome Sequence of a Novel Porcine Sapelovirus Strain YC2011 Isolated from Piglets with Diarrhea

Cited by 30 publications

References 9 publications

Detection of a novel sapelovirus in central nervous tissue of pigs with polioencephalomyelitis in the USA

Detection of a novel sapelovirus in central nervous tissue of pigs with polioencephalomyelitis in the USA

Rapid and real-time detection of Porcine Sapelovirus by reverse transcription loop-mediated isothermal amplification assay

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