Isolation and characterization of tetramicrosatellite DNA markers in the Eurasian otter (<i>Lutra lutra</i>)

Huang, Chuan; Hsu, Yu‐Kuei; Lee, Ling Ling; Li, Shou Hsien

doi:10.1111/j.1471-8286.2005.00912.x

Cited by 31 publications

(24 citation statements)

References 9 publications

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“…Subsequently because of the loss of released individuals, the occurrence of offspring and the increasing relatedness among individuals, we had to gradually increase the number of microsatellite loci: OT04, OT05, OT07, OT14, OT17, OT19 and OT22 (Huang et al 2005) and RI18 (Beheler et al 2005). We only used tetranucleotide microsatellite loci to reduce the occurrence of stutter bands and ambiguity in scoring that often happens with dinucleotide loci.…”

Section: Genetic Typingmentioning

confidence: 99%

The reintroduction of the Eurasian otter (Lutra lutra) into the Netherlands: hidden life revealed by noninvasive genetic monitoring

et al. 2010

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Section: Genetic Typingmentioning

confidence: 99%

The reintroduction of the Eurasian otter (Lutra lutra) into the Netherlands: hidden life revealed by noninvasive genetic monitoring

et al. 2010

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“…In particular, we genotyped six loci 'Lut' (set1: Lut453, Lut604, Lut701, Lut832, Lut833 and Lut902; Dallas et al 1998) already used in many previous studies (Dallas et al 2002;Kalz et al 2006;Hájková et al 2009) and seven loci 'OT' (set2: OT04, OT05, OT07, OT14, OT17, OT19, OT22; Huang et al 2005) only recently used for L. lutra (Koelewijn et al 2010;Quaglietta et al 2013), but never explicitly compared to Lut loci. Due to the low genetic variability of our small otter population, we had to use both sets of loci for individual recognition.…”

Section: Dna Amplification and Genotypingmentioning

confidence: 99%

Procedures to genotype problematic non-invasive otter (Lutra lutra) samples

et al. 2014

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Non-invasive genetics is a powerful tool in wildlife research and monitoring, especially when dealing with elusive\ud and rare species such as the Eurasian otter (Lutra lutra). Nevertheless, otter DNA obtained from scats and anal secretions\ud appears to be exposed to very quick degradation processes, and the success rate in DNA amplification is lower\ud than in other carnivores.We collected 191 samples from April to September 2011 along the river Sangro basin (Italy) which was recently re-colonized by the Eurasian otter. Using two sets of microsatellite loci (six Lut and seven OT loci), we investigated the influence of sample type and age, collection time, storage time, temperature and humidity on genotyping success and amplification success. We also tested the efficacy of different DNA extraction kits and storage buffer mediums. Finally, we compared amplification success rate, allelic dropout and false allele rates for each locus. We obtained a mean amplification success rate of 79.0% and a genotyping success rate of 35.1 %. Fresh pure jellies yielded the highest amplification success and genotyping rate. Six microsatellite loci should be theoretically sufficient to istinguish the individual unrelated otters (PID=0.001), while 13 loci were needed to distinguish sibling otters (PIDsibs=0.002) in our population. We identified 11 otters, and molecular sexing ascertained the presence of five males, four females and two uncertain individuals. Generalized linear models highlighted a significant influence of sample type and age, temperature and humidity both on genotyping and amplification success

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“…Thirteen microsatellite loci identified in the Eurasian otter, Lutra lutra, [Lut435, Lut453, Lut604, Lut701, Lut733 and Lut782 (Dallas and Piertney 1998); 04OT17 (Huang et al 2005)] and North American river otter, Lontra canadensis, [RIO3 and RIO6 (Beheler et al 2004); RIO12, RIO15, RIO16 and RIO17 (Beheler et al 2005)] were used in this study. These markers had previously been PCR amplified in the giant otter and were found to be polymorphic (Pickles et al 2009).…”

Section: Dna Extraction and Amplificationmentioning

confidence: 99%

Genetic diversity and population structure in the endangered giant otter, Pteronura brasiliensis

et al. 2011

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We assessed levels of genetic diversity and investigated patterns of population structure in three remnant populations of the endangered giant otter, Pteronura brasiliensis, using microsatellite loci. All populations displayed moderate to low levels of heterozygosity and allelic richness (H O 0.56-0.57, A R 4.00-5.15) and effective population sizes were low (N E 10.8-54) although only the Iténez population exhibited the signature of a genetic bottleneck. Population structure analyses revealed a pattern in which the populations of the Upper Amazon, Orinoco and Essequibo drainages comprised partially differentiated segments of a northern South American metapopulation, whereas the population of the Iténez appeared isolated. The observed patterns are congruent with previous mitochondrial DNA analysis which suggested the Iténez and northern South American groups constitute two evolutionary significant units. The results presented here should be considered in planning future policies aiming to manage the recovery of the giant otter across its range.

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Isolation and characterization of tetramicrosatellite DNA markers in the Eurasian otter (Lutra lutra)

Cited by 31 publications

References 9 publications

The reintroduction of the Eurasian otter (Lutra lutra) into the Netherlands: hidden life revealed by noninvasive genetic monitoring

The reintroduction of the Eurasian otter (Lutra lutra) into the Netherlands: hidden life revealed by noninvasive genetic monitoring

Procedures to genotype problematic non-invasive otter (Lutra lutra) samples

Genetic diversity and population structure in the endangered giant otter, Pteronura brasiliensis

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