Isolation and characterization of ten microsatellite loci in the catfish Trichomycterus areolatus (Siluriformes: Trichomycteridae), with cross-amplification in seven Trichomycterinae species

Muñoz‐Rojas, Pablo; Quezada‐Romegialli, Claudio; Véliz, David

doi:10.1007/s12686-011-9569-2

Cited by 5 publications

(3 citation statements)

References 5 publications

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“…These microsatellites were amplified following the protocol described by Muñoz, Quezada‐Romegialli, and Véliz (2011). The 10 microsatellite loci (TR01, TR04, TR06, TR10, TR11, TR14, TR17, TR21, TR22 and TR24) described by Muñoz‐Rojas, Quezada‐Romegialli, and Véliz (2012) were screened for the catfish T. areolatus . All loci were amplified in single reactions.…”

Section: Methodsmentioning

confidence: 99%

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Dammed river: Short‐ and long‐term consequences for fish species inhabiting a river in a Mediterranean climate in central Chile

Vega‐Retter

Muñoz‐Rojas

Rojas‐Hernández

et al. 2020

Aquatic Conservation

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1. The presence of a dam disturbs river flow, which in turn directly affects the communities and evolutionary potential of riverine species. To detect the ecological effects of a dam on genetic diversity, genetic structure, and their progress in time, two riverine fishes living upstream and downstream of an irrigation reservoir were studied at two periods after its construction in 2004 in central Chile. 2. Samples of the Neotropical silverside Basilichthys microlepidotus and the pencil catfish Trichomycterus areolatus were obtained 2 and 7 years after the Corrales Dam was built. The microsatellite DNA variability of both species upstream and downstream of the dam was analysed. 3. Fish analysed 2 years after dam construction did not show genetic differences between upstream and downstream populations; however, fish obtained 7 years after dam construction showed differences between populations above and below the dam and differences from individuals collected 5 years earlier. 4. The current effective population sizes of both species were smaller in samples obtained upstream than in samples obtained downstream. Simulations showed a migration equal to zero as most probable after reservoir construction, suggesting that the dam is an impermeable barrier to the movement of individuals of these species. These results showed that population fragmentation in time could be related to the barrier imposed by the dam. 5. In a scenario of no new contact between populations located upstream and downstream of the dam, the simulation predicts a reduction of genetic diversity ranging from 3.98 to 8.09% over the next 60 years. 6. Analyses suggest that the Corrales Reservoir may be affecting the evolutionary potential of the populations upstream and downstream from the dam.

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Section: Methodsmentioning

confidence: 99%

“…The 10 microsatellite loci (TR01, TR04, TR06, TR10, TR11, TR14, TR17, TR21, TR22 and TR24) described by Muñoz-Rojas, Quezada-Romegialli, and Véliz (2012) were screened for the catfish T. areolatus.…”

Section: Genetic Analysismentioning

confidence: 99%

Dammed river: Short‐ and long‐term consequences for fish species inhabiting a river in a Mediterranean climate in central Chile

Vega‐Retter

Muñoz‐Rojas

Rojas‐Hernández

et al. 2020

Aquatic Conservation

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“…There are limited transcriptomic resources available for fish native to South America, and none available of the Trichomycterids. Current molecular research with this organism is limited, and includes a karyotype 15 , seasonal variation in biomarkers 8 , local industrial discharge impacts 6 , agricultural disturbances 16 , microsatellite loci for conservation resources 17 , and population genetics 18 .…”

Section: Introductionmentioning

confidence: 99%

De novo Assembly and Analysis of the Chilean Pencil Catfish Trichomycterus areolatus Transcriptome

et al. 2016

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Trichomycterus areolatus is an endemic species of pencil catfish that inhabits the riffles and rapids of many freshwater ecosystems of Chile. Despite its unique adaptation to Chile's high gradient watersheds and therefore potential application in the investigation of ecosystem integrity and environmental contamination, relatively little is known regarding the molecular biology of this environmental sentinel. Here, we detail the assembly of the Trichomycterus areolatus transcriptome, a molecular resource for the study of this organism and its molecular response to the environment. RNA-Seq reads were obtained by next-generation sequencing with an Illumina® platform and processed using PRINSEQ. The transcriptome assembly was performed using TRINITY assembler. Transcriptome validation was performed by functional characterization with KOG, KEGG, and GO analyses. Additionally, differential expression analysis highlights sex-specific expression patterns, and a list of endocrine and oxidative stress related transcripts are included.

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Characterization of Genetic Resources

Hilsdorf

Hallerman

2017

Genetic Resources of Neotropical Fishes

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Isolation and characterization of ten microsatellite loci in the catfish Trichomycterus areolatus (Siluriformes: Trichomycteridae), with cross-amplification in seven Trichomycterinae species

Cited by 5 publications

References 5 publications

Dammed river: Short‐ and long‐term consequences for fish species inhabiting a river in a Mediterranean climate in central Chile

Dammed river: Short‐ and long‐term consequences for fish species inhabiting a river in a Mediterranean climate in central Chile

De novo Assembly and Analysis of the Chilean Pencil Catfish Trichomycterus areolatus Transcriptome

Characterization of Genetic Resources

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