Isolation and characterization of TaQsd1 genes for period of dormancy in common wheat (Triticum aestivum L.)

Wei, Wenxin; Min, Xiaoyu; Shan, Siyao; Jiang, Hao; Cao, Junhui; Li, Li; Wang, Jianfeng; Wang, Shengxing; Zhu, Yanfang; Lu, Jie; Si, Hongqi; Xia, Xianchun; Ma, Chunyan; Zhang, Haiping; Chang, Cheng

doi:10.1007/s11032-019-1060-x

Cited by 20 publications

(11 citation statements)

References 105 publications

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“…The Qsd1 gene encodes alanine aminotransferase and is an important gene controlling dormancy in barley grains. In our study, TaQsd1 was obtained by homologous cloning in wheat, and its involvement in the regulation of seed dormancy was confirmed [ 47 ]. In a previous study, CRISPR-Cas9 technology was used to edit TaQsd1 , and the genetically edited wheat had a significantly longer dormancy period and improved PHS resistance compared with the wild type [ 67 ].…”

Section: Progress In Breeding Phs-resistant Wheat In Chinamentioning

confidence: 94%

“…To date, only a few functional genes have been identified for PHS resistance. So far, seven genes have been isolated through homologous cloning and mapping cloning: TaVp-1 [ 38 , 39 , 40 , 41 , 42 ], TaDOG1L1 [ 43 ], TaMFT / TaPHS1 [ 8 , 44 ], TaSdr [ 45 ], TaMKK3 [ 46 ], TaQsd1 / TaAlaAT [ 47 ], and Myb10-D [ 6 ] ( Table 2 ). These genes mainly participate in the regulation of seed dormancy by affecting the ABA/GA synthesis/metabolism/signaling pathways, thereby affecting PHS resistance.…”

Section: The Regulation Genes For Phs Resistancementioning

confidence: 99%

“…Therefore, gene editing may be an efficient breeding technique for improving wheat PHS resistance. We also identified some genes and their dominant alleles for PHS resistance, including TaVp-1A ( TaVp-1Ab , TaVp-1Ad ), TaVp-1B ( TaVp-1Bb , TaVp-1Bc , TaVp-1Bd , TaVp-1Be , and TaVp-1Bf ), TaMFT-3A ( TaMFT-3Aa ), and TaQsd1 ( TaQsd1-5Ba ) [ 39 , 40 , 41 , 47 , 48 ]. Simultaneously, some candidate genes, such as TaGASR34 , TaC3Hs , TaGATAs , TaVQs , TaIQDs and TaGA20ox1 , were identified successively in our study, and can be considered as important gene resources for transgenic breeding or gene editing/breeding for improving wheat PHS resistance after further verifying their functions [ 50 , 51 , 52 , 53 , 54 , 55 , 56 ].…”

Section: Progress In Breeding Phs-resistant Wheat In Chinamentioning

confidence: 99%

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Genetic Improvement of Wheat with Pre-Harvest Sprouting Resistance in China

Chang

Zhang

et al. 2023

Genes

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Wheat pre-harvest sprouting (PHS) refers to the germination of seeds directly on the spike due to rainy weather before harvest, which often results in yield reduction, quality deterioration, and seed value loss. In this study, we reviewed the research progress in the quantitative trait loci (QTL) detection and gene excavation related to PHS resistance in wheat. Simultaneously, the identification and creation of germplasm resources and the breeding of wheat with PHS resistance were expounded in this study. Furthermore, we also discussed the prospect of molecular breeding during genetic improvement of PHS-resistant wheat.

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Section: Progress In Breeding Phs-resistant Wheat In Chinamentioning

confidence: 94%

Section: The Regulation Genes For Phs Resistancementioning

confidence: 99%

Section: Progress In Breeding Phs-resistant Wheat In Chinamentioning

confidence: 99%

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Genetic Improvement of Wheat with Pre-Harvest Sprouting Resistance in China

Chang

Zhang

et al. 2023

Genes

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“…Lastly, Cluster 5 genes were specifically expressed at DAP22 and were related to seed dormancy, e.g. TaQsd1 (Wei et al, 2019) and TaPPO-2A (Beecher and Skinner, 2011) (Fig. 1, D to F).…”

Section: Transcriptome and Chromatin Landscapes During Grain Developm...mentioning

confidence: 99%

Unraveling wheat endosperm development: epigenetic regulation and novel regulators for enhanced yield and quality

Zhao,

Chen,

Zhang

et al. 2024

Preprint

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Starch content and seed storage protein (SSP) composition are critical factors influencing wheat grain yield and quality. To uncover the molecular mechanisms governing their biosynthesis, we conducted transcriptome and epigenome profiling across key endosperm developmental stages, revealing that chromatin accessibility, H3K27ac, and H3K27me3 collectively regulate SSP and starch genes with varying impact. Population transcriptome and phenotype analyses highlighted the crucial role of accessible promoter regions as a genetic variation resource, influencing grain yield and quality in a core collection of wheat accessions. By integrating time-serial RNA-seq and ATAC-seq data, we constructed a hierarchical transcriptional regulatory network (TRN) governing starch and SSP biosynthesis, identifying 42 high-confidence novel candidates. These candidates exhibited overlap with genetic regions associated with grain size and quality traits, and their functional significance was validated through expression-phenotype association analysis among wheat accessions and TILLING mutants. In-depth functional analysis ofwheat abscisic acid insensitive 3-A1(TaABI3-A1) with genome editing knock-out lines demonstrated its role in promoting SSP accumulation while repressing starch biosynthesis through transcriptional regulation. An elite haplotype ofTaABI3-A1with higher grain weight was identified during the breeding process in China, and its superior trait was associated with alteredTaABI3-A1expression levels. Additionally, we identified the potential upstream regulator, wheat GAGA-binding transcription factor 1 (TaGBP1), influencingTaABI3-A1expression. Our study provides novel and high-confidence regulators, presenting an effective strategy for understanding the regulation of SSP and starch biosynthesis and contributing to breeding enhancement.

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“…Until now, seven genes controlling SD and PHS resistance have been identified through map- or homology-based cloning in wheat, namely TaVp-1 ( Yang et al., 2007 ), TaDOG1L1 ( Ashikawa et al., 2014 ), TaMFT/TaPHS1 ( Nakamura et al., 2011 ; Liu et al., 2013 ), TaSdr ( Zhang et al., 2014 ; Zhang et al., 2017 ), TaMKK3 ( Torada et al., 2016 ), TaQsd1 ( Wei et al., 2019 ) and Myb10-D ( Lang et al., 2021 ). Among them, TaDOG1L1 is a wheat homolog of the Arabidopsis dormancy gene DOG1 ( Delay of Germination 1 ).…”

Section: Introductionmentioning

confidence: 99%

Identification and validation of coding and non-coding RNAs involved in high-temperature-mediated seed dormancy in common wheat

et al. 2023

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IntroductionSeed dormancy (SD) significantly decreases under high temperature (HT) environment during seed maturation, resulting in pre-harvest sprouting (PHS) damage under prolonged rainfall and wet weather during wheat harvest. However, the molecular mechanism underlying HT-mediated SD remains elusiveSeed dormancy (SD) significantly decreases under high temperature (HT) environment during seed maturation, resulting in pre-harvest sprouting (PHS) damage under prolonged rainfall and wet weather during wheat harvest. However, the molecular mechanism underlying HT-mediated SD remains elusive.MethodsHere, the wheat landrace ‘Waitoubai’ with strong SD and PHS resistance was treated with HT from 21 to 35 days post anthesis (DPA). Then, the seeds under HT and normal temperature (NT) environments were collected at 21 DPA, 28 DPA, and 35 DPA and subjected to whole-transcriptome sequencing.ResultsThe phenotypic data showed that the seed germination percentage significantly increased, whereas SD decreased after HT treatment compared with NT, consistent with the results of previous studies. In total, 5128 mRNAs, 136 microRNAs (miRNAs), 273 long non-coding RNAs (lncRNAs), and 21 circularRNAs were found to be responsive to HT, and some of them were further verified through qRT-PCR. In particular, the known gibberellin (GA) biosynthesis gene TaGA20ox1 (TraesCS3D02G393900) was proved to be involved in HT-mediated dormancy by using the EMS-mutagenized wheat cultivar Jimai 22. Similarly, a novel gene TaCDPK21 (TraesCS7A02G267000) involved in the calcium signaling pathway was validated to be associated with HT-mediated dormancy by using the EMS mutant. Moreover, TaCDPK21 overexpression in Arabidopsis and functional complementarity tests supported the negative role of TaCDPK21 in SD. We also constructed a co-expression regulatory network based on differentially expressed mRNAs, miRNAs, and lncRNAs and found that a novel miR27319 was located at a key node of this regulatory network. Subsequently, using Arabidopsis and rice lines overexpressing miR27319 precursor or lacking miR27319 expression, we validated the positive role of miR27319 in SD and further preliminarily dissected the molecular mechanism of miR27319 underlying SD regulation through phytohormone abscisic acid and GA biosynthesis, catabolism, and signaling pathways.DiscussionThese findings not only broaden our understanding of the complex regulatory network of HT-mediated dormancy but also provide new gene resources for improving wheat PHS resistance to minimize PHS damage by using the molecular pyramiding approach.

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Isolation and characterization of TaQsd1 genes for period of dormancy in common wheat (Triticum aestivum L.)

Cited by 20 publications

References 105 publications

Genetic Improvement of Wheat with Pre-Harvest Sprouting Resistance in China

Genetic Improvement of Wheat with Pre-Harvest Sprouting Resistance in China

Unraveling wheat endosperm development: epigenetic regulation and novel regulators for enhanced yield and quality

Identification and validation of coding and non-coding RNAs involved in high-temperature-mediated seed dormancy in common wheat

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