Genetic Improvement of Wheat with Pre-Harvest Sprouting Resistance in China

Chang, Cheng; Zhang, Haiping; Lu, Jie; Si, Hongqi; Ma, Chunyan

doi:10.3390/genes14040837

Cited by 6 publications

(4 citation statements)

References 61 publications

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“…Molecular marker-assisted selection was effectively used to improve the efficiency and accuracy in breeding for resistance to PHS, creating wheat varieties such as Zhongmai 911 and Annong 0711. Genetic transformation and gene editing were also studied to improve the resistance to PHS; the results showed application potential, but no breeding varieties have been released for wheat production [ 19 ].…”

Section: Editorialmentioning

confidence: 99%

Editorial for the Special Issue “Genetics Studies on Wheat”

2023

Genes

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Section: Editorialmentioning

confidence: 99%

Editorial for the Special Issue “Genetics Studies on Wheat”

2023

Genes

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“…Increasing temperatures may increase the risk of LMA and PHS [36,37]. Increasing climate variability appears to be associated with more frequent Falling Number problems in wheat [37][38][39][40][41][42][43]. To prevent wheat end-use quality problems due to α-amylase, it is essential to have more accessible, rapid, and isoform-specific tests than the Falling Number method for determining if grain samples are sound, and if not, the degree of loss in quality caused.…”

Section: Overviewmentioning

confidence: 99%

Development of Novel Monoclonal Antibodies to Wheat Alpha-Amylases Associated with Grain Quality Problems That Are Increasing with Climate Change

Hauvermale,

Matzke,

Bohaliga

et al. 2023

Plants

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Accurate, rapid testing platforms are essential for early detection and mitigation of late maturity α-amylase (LMA) and preharvest sprouting (PHS) in wheat. These conditions are characterized by elevated α-amylase levels and negatively impact flour quality, resulting in substantial economic losses. The Hagberg–Perten Falling Number (FN) method is the industry standard for measuring α-amylase activity in wheatmeal. However, FN does not directly detect α-amylase and has major limitations. Developing α-amylase immunoassays would potentially enable early, accurate detection regardless of testing environment. With this goal, we assessed an expression of α-amylase isoforms during seed development. Transcripts of three of the four isoforms were detected in developing and mature grain. These were cloned and used to develop E. coli expression lines expressing single isoforms. After assessing amino acid conservation between isoforms, we identified peptide sequences specific to a single isoform (TaAMY1) or that were conserved in all isoforms, to develop monoclonal antibodies with targeted specificities. Three monoclonal antibodies were developed, anti-TaAMY1-A, anti-TaAMY1-B, and anti-TaAMY1-C. All three detected endogenous α-amylase(s). Anti-TaAMY1-A was specific for TaAMY1, whereas anti-TaAMY1-C detected TaAMY1, 2, and 4. Thus, confirming that they possessed the intended specificities. All three antibodies were shown to be compatible for use with immuno-pulldown and immuno-assay applications.

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“…Furthermore, a multitude of dormancy-or PHS-associated quantitative trait loci (QTLs) have been identified on each chromosome of wheat [18,19]. Taking chromosome 6B as an example, molecular markers wmc104 [20] and wPt0959 [21] on the short arm of the chromosome were reported to be associated with tolerance to PHS in a recombinant inbred line (RIL) population and a set of 96 winter wheat accessions, respectively.…”

Section: Introductionmentioning

confidence: 99%

Mapping of a Major-Effect Quantitative Trait Locus for Seed Dormancy in Wheat

Gao,

Qiao,

Mei

et al. 2024

IJMS

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The excavation and utilization of dormancy loci in breeding are effective endeavors for enhancing the resistance to pre-harvest sprouting (PHS) of wheat varieties. CH1539 is a wheat breeding line with high-level seed dormancy. To clarify the dormant loci carried by CH1539 and obtain linked molecular markers, in this study, a recombinant inbred line (RIL) population derived from the cross of weak dormant SY95-71 and strong dormant CH1539 was genotyped using the Wheat17K single-nucleotide polymorphism (SNP) array, and a high-density genetic map covering 21 chromosomes and consisting of 2437 SNP markers was constructed. Then, the germination percentage (GP) and germination index (GI) of the seeds from each RIL were estimated. Two QTLs for GP on chromosomes 5A and 6B, and four QTLs for GI on chromosomes 5A, 6B, 6D and 7A were identified. Among them, the QTL on chromosomes 6B controlling both GP and GI, temporarily named QGp/Gi.sxau-6B, is a major QTL for seed dormancy with the maximum phenotypic variance explained of 17.66~34.11%. One PCR-based diagnostic marker Ger6B-3 for QGp/Gi.sxau-6B was developed, and the genetic effect of QGp/Gi.sxau-6B on the RIL population and a set of wheat germplasm comprising 97 accessions was successfully confirmed. QGp/Gi.sxau-6B located in the 28.7~30.9 Mbp physical position is different from all the known dormancy loci on chromosomes 6B, and within the interval, there are 30 high-confidence annotated genes. Our results revealed a novel QTL QGp/Gi.sxau-6B whose CH1539 allele had a strong and broad effect on seed dormancy, which will be useful in further PHS-resistant wheat breeding.

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Genetic Improvement of Wheat with Pre-Harvest Sprouting Resistance in China

Cited by 6 publications

References 61 publications

Editorial for the Special Issue “Genetics Studies on Wheat”

Editorial for the Special Issue “Genetics Studies on Wheat”

Development of Novel Monoclonal Antibodies to Wheat Alpha-Amylases Associated with Grain Quality Problems That Are Increasing with Climate Change

Mapping of a Major-Effect Quantitative Trait Locus for Seed Dormancy in Wheat

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