Isolation and characterization of starch degrading bacteria from disparate soil samples

Abstract

Amylases are starch degrading enzymes. These enzymes play a pivotal role in the biotechnology industries in food, fermentation, textiles, and paper production. Many industries lack local amylase supply, so the demand for amylase is often high. The study focuses on the screening and isolation of amylase producing bacteria from disparate samples of soil. The promising hydrolytic strains of bacteria from the collected samples were characterized using enzyme kinetics and further investigation is carried out to characterize the amylase enzyme produced by optimization of pH and temperature. The shortlisted isolate was identified using 16s rRNA sequencing. This study is an initial result of an exploration where soil is a source of industrially important amylase producing bacterial strains.

Antimicrobial peptide coding gene of thermophilic bacteria isolated from crater hot spring in mountains around West Java

“…The PCR started with 5 min denaturation at 95°C, followed by 35 cycles of 30 s denaturation at 94°C, 30 sec annealing at 60°C, 1 min extension at 72°C, and 10 min final extension at 72°C [17]. A total of 5 µL of the product were analyzed using 1% agarose gel dissolved in 1X TAE buffer, and the gel electrophoresis was carried out for 25 min at 90 V. The presence of the DNA band was visualized and observed under UV light using a gel documentation system [18]. Each isolate's PCR product was then compared to a 1 kb DNA Ladder (Promega).…”