Isolation and characterization of some polycyclic aromatic hydrocarbon degrading bacteria from Nsukka soils in Nigeria

Abstract

Twenty-four bacteria capable of utilizing naphthalene, as their sole source of carbon and energy for growth were isolated from three different sites in Nsukka, Nigeria. By standard bacteriological methods, these bacteria were characterized taxonomically as belonging to the genus Pseudomonas, Burkholderia or Actinomycetes. Two of the isolates, which showed the highest growth during screening as demonstrated by an increase in their optical densities (OD 600 ) and identified as Pseudomonas aeruginosa and Burkholderia cepacia respectively, were also able to grow in anthracene and carbazole, but not very much so in 2,4-dichlorophenol and D-camphor. The isolates showed a concentration-dependent growth in all the compounds they grew in. There were visible changes in the colour of the growth medium of the isolates during their incubation, suggesting the production of different metabolites. There were also changes in their medium pH during growth. These studies demonstrate the possession by the bacterial species of novel degradative systems.

Comparison of biostimulation and bioaugmentation for remediation of soil contaminated with spent motor oil

“…The bacteria (Pseudomonas aeruginosa (Kiyohara et al, 1992;Nnamchi et al, 2006) and Bacillus subtilis ( Abdulsalam and Omale, 2009) and the Fertilizer (NPK 20:10:10) used were obtained from the National Veterinary Research Institute Vom, Plateau State-Nigeria and Bauchi Fertilizer Company Limited, Bauchi-Nigeria respectively. In addition, potassium dihydrogen orthophosphate (K 2 HPO 4 ) used was of analytical grade.…”

Levels of polycyclic aromatic hydrocarbons and polychlorinated biphenyls in sediment of bitumen deposit impacted area

“…The carbon atoms are arranged in a series of adjoining sixmembered rings. Their biochemical persistence in the environment arises from dense clouds of π electrons on both sides of the ring structures making them resistant to neucleophilic attack (Jonsen et al, 2005;Nnamchi et al, 2006). PAHs have been documented to cause several health problems (Zhang et al, 2009).…”

Búsqueda De Capacidad Productora De Biosurfactantes en Actinobacterias Haloalcalófilas Y Haloalcalotolerantes

“…Se incubaron a 37 ºC y a 150 rpm. El crecimiento microbiano se determinó al medir su densidad óptica a 600 nm (Nnamchi et al 2006) en un espectrofotómetro (Perkin-Elmer UV-Vis, modelo 551S) a diferentes tiempos hasta alcanzar la fase estacionaria.…”

“…Este mL fue transferido a un matraz con tapón de rosca cubierto con papel aluminio que contenía 60 mL de MSM-Ant. Los matraces fueron incubados a 150 rpm y 37 o C. El crecimiento microbiano se determinó midiendo su densidad óptica a 600 nm (Nnamchi et al 2006) en un espectrofotómetro (Perkin-Elmer UV-Vis, modelo 551S) a diferentes tiempos hasta alcanzar la fase estacionaria.…”