Isolation and characterization of soluble sulfated polysaccharide from the red seaweed Gracilaria cornea

Melo, Márcia Rúbia Silva; Feitosa, Judith P.A.; Freitas, Ana Lúcia Ponte; Paula, Regina C.M. de

doi:10.1016/s0144-8617(02)00006-1

Cited by 267 publications

(100 citation statements)

References 29 publications

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“…Therefore, the peaks obtained in Hf1s, Hf2s and Hf3s ranged from 8.0 × 10 2 to 5.0 × 10 6 g/mol. These results suggested that H. floresia Hf2s behaved as heterogeneous system similar to other natural polysaccharides identified from Gracilaria cornea (Rhodophyta) (Melo et al, 2002) and Ulva rotundata (Chlorophyta) (Robic et al, 2009). In fact, algae polysaccharides have MM average and with the distribution of molecular species similar in its structures, but their variations occur in the same backbone polysaccharide size (Stephen, 1995).…”

Section: Molar Mass Distributionsupporting

confidence: 58%

“…A differential refractometer and an ultraviolet photometer (at 280 nm) were used as detectors, and the elution volume was corrected to the internal marker of ethylene glycol at 11.25 mL. Pullulan samples (Shodex Denko) of M w 5.9 × 10 3 , 1.18 × 10 4 , 4.73 × 10 4 , 2.12 × 10 5 and 7.88 × 10 5 g/mol were used as standards (Melo et al, 2002).…”

Section: Molar Mass Distributionmentioning

confidence: 99%

“…Sulfated polysaccharides (SPs) make up a group of biopolymers that occur in a great variety of marine organisms (Stephen, 1995;Hayashi et al, 1996;Mourão and Pereira, 1999;Aquino et al, 2005;Pomin and Mourão, 2008;Robic et al, 2009;Rodrigues et al, 2009a;Mestechkina and Shcherbukhin, 2010;Rodrigues et al, 2010). In red seaweed, SPs are known as sulfated galactans, and are found in carrageenans and agarans (Melo et al, 2002;Marinho-Soriano, 2006;Pomin and Mourão, 2008;Campo et al, 2009;Silva et al, 2010;Rodrigues et al, 2011a). These highly charged macromolecules (presence of sulfate groups) exhibit chemical structures of complex and heterogeneous nature, playing an important role in ionic, mechanical and osmotic functions, and are constituents of the extracellular matrix of marine algae (Kloareg and Quatrano, 1988).…”

Section: Introductionmentioning

confidence: 99%

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Effect of a crude sulfated polysaccharide from Halymenia floresia (Rhodophyta) on gastrointestinal smooth muscle contractility

Graça

Bezerra

Lima

et al. 2011

Braz. arch. biol. technol.

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Section: Molar Mass Distributionsupporting

confidence: 58%

Section: Molar Mass Distributionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of a crude sulfated polysaccharide from Halymenia floresia (Rhodophyta) on gastrointestinal smooth muscle contractility

Graça

Bezerra

Lima

et al. 2011

Braz. arch. biol. technol.

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“…It is also supported by data that show polysaccharides from Gigartina skottsbergii, Tichocarpus crinitus, Gloiopeltis furcate, Chondrus crispus, and Halymenia durvillei, Geogiella confluens, and Cryptonemia seminervis are sulfated 15−42% (Barabanova et al 2005;Fenoradosoa et al 2009;Kolender and Matulewicz 2002;Mendes et al 2014;McCandless et al 1973;Piriz and Cerezo 1991;Yu et al 2010). In contrast, low sulfate content was detected in acidic polysaccharides from other red seaweed: 2.8% from P. decipiens (Matsuhiro and Urzua 1996), 8.4% from Grarcilaria birdiae (Souza et al 2012), 4.8% from Gracilaria cornea (Melo et al 2002), 1.2% from Gracilaria dura (Marinho-Soriano and Bourret 2005), and 5.08% from H. musciformis (Sousa et al 2016). The monosaccharide composition of SP1 and SP2 analyzed by Values were obtained by setting the sum of each mole number at 100% HPAEC-PAD revealed that galactose is the major monosaccharide (92.9% and 91.3% in mole %), while fucose (6.5% and 7.4%), glucose (1.8% and 0.6%), and xylose (0.9% and 0.7%) are present as minor components (Table 3 and Fig.…”

Section: Chemical Composition Of Spsmentioning

confidence: 65%

Anticancer Activity of Sulfated Polysaccharides Isolated from the Antarctic Red Seaweed Iridaea cordata

Kim¹,

Kim²,

Koo³

et al. 2016

Ocean and Polar Research

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: This study aimed to isolate and characterize sulfated polysaccharides (SPs) from Iridaea cordata and evaluate their anticancer activity. SPs of the Antarctic red seaweed were obtained by CaCl 2 (SP1) and ethanol precipitations (SP2) following diluted acid extraction at room temperature. Yields of SP1 and SP2 were approximately 14% and 23%, respectively, of the dry weight of red seaweed. The average molecular mass of the SP1 and SP2 was estimated about 1.84 × 10 3 and 1.42 × 10 3 kDa, respectively, by size-fractionation High-Performance Liquid Chromatography (HPLC). From the High-Performance AnionExchange Chromatography-Pulsed Amperometric Detection (HPAEC-PAD) analysis, the main monosaccharide was galactose with glucose and fucose as minor components. The sulfate content of SP2 (40.4%) was slightly higher than that of SP1 (33.8%). The FT-IR spectra also showed characteristic band of carrageenan-like sulfated polysaccharides. Taken together the SPs are thought to be carrageenan-like sulfated galactan. The polysaccharides (SPs) from I. cordata exhibited weak antitumor activity against PC-3 (prostate cancer), HeLa (cervical cancer), and HT-29 (human colon adenocarcinoma). To our knowledge, this is the first data on biological activity of the Antarctic red seaweed I. cordata.

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“…Sulfated polysaccharides are complex and heterogeneous macromolecules found in diverse marine aquatic organisms, including animal (POMIN; WU et al, 2010), marine algae GHOSH et al, 2004;AZEVEDO et al, 2009;RODRIGUES et al, 2011) and sea grasses (AQUINO et al, 2005) tissues, and their biological activities have been recognized in marine algae (FARIAS et al, 2000;MELO et al, 2004;PEREIRA et al, 2005;FONSECA et al, 2008;RODRIGUES et al, 2009a; Acta Scientiarum. Biological Sciences Maringá, v. 33, n. 3, p. 255-261, 2011 LINS et al, 2009;RODRIGUES et al, 2010), fishes (RODRIGUES et al, 2009b) and marine invertebrates (WU et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Anticoagulant properties of a crude sulfated polysaccharide from the red marine alga Halymenia floresia (Clemente) C. Agardh

Amorim

Rodrigues

Araújo

et al. 2011

Acta Sci. Biol. Sci.

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ABSTRACT. Alternative sources of anticoagulants have arisen as a result of the increasing demand for safer anticoagulant clinical therapy, and the sulfated polysaccharides of seaweeds have gained attention in biomedicine. In this study, crude sulfated polysaccharide fractions (denominated Hf1, Hf2 and Hf3) were obtained from the red marine alga Halymenia floresia and the anticoagulant properties of a soluble crude polysaccharide fraction (Hf2s) were assayed. The three differential extractions yielded 38.6%. The polysaccharides are composed mainly of galactose, with small amounts of xylose and glucose. The anticoagulant properties of Hf2s containing 53.8% sulfate and 3% protein was also compared to those of heparin ) by assays of activated partial thromboplastin time (APTT) and thrombin time (TT) using normal human plasma. Hf2s showed a higher anticoagulant activity (68.4 IU mg -1 ) than those of Hf1s and Hf3s, whose activities were 37.6 and 36.6 IU mg -1 , respectively. The compound was less active than heparin, but its anticoagulant mechanism suggested that it is dependent on cofactor heparin II to inhibit thrombin activity, but not on cofactors VIII and IX. Therefore, the polysaccharide from H. floresia interfered on coagulation cascade. RESUMO. Propriedades anticoagulantes de um polissacarídeo sulfatado bruto da alga marinha vermelha Halymenia floresia (Clemente) C. Agardh. O aumento da demanda por anticoagulantes para a terapia clínica tem motivado a busca por fontes alternativas de anticoagulantes mais seguros e os polissacarídeos sulfatados de algas marinhas têm ganhado atenção na biomedicina. Objetivou-se obter frações de polissacarídeos sulfatados brutos (denominadas Hf1; Hf2 e Hf3) da alga marinha vermelha Halymenia floresia e para avaliar as propriedades anticoagulantes de uma fração polissacarídica bruta solúvel anticoagulante (Hf2s). As três extrações diferenciais renderam 38,60%. Os polissacarídeos são principalmente compostos de galactose com pequenas quantidades de xilose e glucose. As propriedades anticoagulantes da Hf2s, contendo 53,80% de sulfato e 3% de proteínas, foram também comparadas com a heparina (193,

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Isolation and characterization of soluble sulfated polysaccharide from the red seaweed Gracilaria cornea

Cited by 267 publications

References 29 publications

Effect of a crude sulfated polysaccharide from Halymenia floresia (Rhodophyta) on gastrointestinal smooth muscle contractility

Effect of a crude sulfated polysaccharide from Halymenia floresia (Rhodophyta) on gastrointestinal smooth muscle contractility

Anticancer Activity of Sulfated Polysaccharides Isolated from the Antarctic Red Seaweed Iridaea cordata

Anticoagulant properties of a crude sulfated polysaccharide from the red marine alga Halymenia floresia (Clemente) C. Agardh

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